Abstract: An automated, portable, and time conservative memory test system for identifying test parameters including type, control line configuration, depth, width, access time, and burst features of any one of a wide variety of synchronous memories including SDRAMs and SGRAMs, and whether an IC chip, bank, board or module, without requiring hardware modifications or additions to the memory device being identified, and without requiring storage of test patterns or characterizing data in the memory device.

Abstract: A magnetic disc storage system includes a magnetic storage disc and a transducing head. During periods of inactivity the transducing head is dithered whereby the head is moved to tracks on the disc according to a number of criteria. The criteria includes wear reduction, power reduction, avoidance of textured zones, head cycling, increased fly height and clearing of debris carried on the head.

Abstract: Methods employing internal oligonucleotide standards in isothermal nucleic acid amplification reactions to determine the efficacy of the amplification reaction and to quantify pre-amplification target levels.

Abstract: A nested loop method for use in a memory test system to identify the width, depth, control line configuration, and part type of a synchronous memory, wherein bit patterns are retrieved from tables representative of a plurality of synchronous memories during execution of nested loops, from outer loop to inner loop, in the order of bank loop, RE loop, CE loop, CS loop, DQMB loop, and part type loop, and bits of an entry of a table occurring after a given entry are either a member of a superset or do not intersect bits of previous entries, and bits of an entry preceding the given entry are either a member of a subset or do not intersect bits of the given entry.

Abstract: The present invention relates to a method for reducing the amount of substances inhibitory to nucleic acid hybridization in samples. The method is practiced prior to release of target nucleic acid from cells of interest and involves contacting the sample with an agent which solubilizes the inhibitory substances and does not effectuate release of nucleic acids from cells in the sample, and then the cells from the agent.

Abstract: Strand Displacement Amplification methods (thermophilic SDA) which can be performed over a broad temperature range (37.degree. C. to 70.degree. C.). The preferred temperature range for thermophilic SDA is 50.degree. C. to 70.degree. C. It has been found that certain thermophilic restriction endonucleases are capable of nicking the hemimodified restriction endonuclease recognition/cleavage site as required by SDA and dissociating from the site. It has further been found that certain thermophilic polymerases are capable of extending from the nick while displacing the downstream strand. Thermophilic SDA, because of reaction temperatures higher than previously possible with conventional SDA enzyme systems, has improved specificity and efficiency, reduced nonspecific background amplification, and potentially improved yields of amplification products.

Abstract: Methods for using reporter mycobacteriophage (RM) and p-nitro-.alpha.-acetylamino-.beta.-hydroxy-propiophenone (NAP) to identify TB complex mycobacteria and distinguish these species from MOTT. RM-infected MOTT show little or no reduction in signal when treated with NAP. In contrast, TB complex mycobacteria infected with RM are distinguishable from RM-infected MOTT by a reduction in signal with NAP treatment.

Abstract: Strand Displacement Amplification methods (thermophilic SDA) which can be performed over a broad temperature range (37.degree. C. to 70.degree. C.). The preferred temperature range for thermophilic SDA is 50.degree. C. to 70.degree. C. It has been found that certain thermophilic restriction endonucleases are capable of nicking the hemimodified restriction endonuclease recognition/cleavage site as required by SDA and dissociating from the site. It has further been found that certain thermophilic polymerases are capable of extending from the nick while displacing the downstream strand. Thermophilic SDA, because of reaction temperatures higher than previously possible with conventional SDA enzyme systems, has improved specificity and efficiency, reduced nonspecific background amplification, and potentially improved yields of amplification products.

Abstract: Mycobacteriophage DS6A has been characterized and found to specifically infect all species of the TB complex, without any detectable infection of mycobacteria species other than those of the TB complex. DNA sequence analysis revealed several potential open reading frames, including one encoding a protein analogous to gp37 of mycobacteriophage L5 and a second encoding a protein with significant homology to the S. coelicolor DNA polymerase .beta. subunit. Based on the DNA sequence analysis, cloning sites can be identified for insertion of reporter genes, making DS6A useful as a reporter phage for specific detection and identification of species of the TB complex.

Abstract: Methods for multiplex amplification of target nucleic acid sequences using a single pair of primers. Defined sequences are appended to the ends of multiple target sequences as part of the amplification reaction so that no steps in addition to amplification are required. The target sequences with the appended defined sequences need not be isolated prior to amplification. In one embodiment for coamplification of two target sequences, a sequence corresponding to a terminal segment of the first target sequence is appended to one end of the second target sequence and a sequence corresponding to a terminal segment of the second target sequence is appended to one end of the first target sequence. Amplification of the two targets then requires only a single pair of primers. Alternatively, a single defined sequence may be appended to the 5' and 3' ends of any number of selected targets. All such modified target sequences may then be amplified using a single pair of primers which hybridize to the defined end-sequences.

Abstract: It has been found that certain glycoproteins, particularly mucins, are inhibitors of nucleic acid amplification reactions and that inhibition of the amplification reaction is associated with partial degradation of the carbohydrate chain. Partial degradation of the carbohydrate of a non-inhibitory glycoprotein renders it inhibitory, and partial degradation of the carbohydrate of a slightly inhibitory glycoprotein makes it more inhibitory. Sample processing prior to amplification may contribute to partial degradation of the carbohydrate chains of the glycoproteins which are present and increase their inhhibitory effect. In contrast, complete removal of the carbohydrate significantly reduces or completely eliminates the inhibitory effect. Methods for reducing or eliminating glycoprotein-associated inhibition of nucleic acid amplification reactions are also disclosed.

Abstract: Mycobacteriophage DS6A has been characterized and found to specifically infect all species of the TB complex, without any detectable infection of mycobacteria species other than those of the TB complex. DNA sequence analysis revealed several potential open reading frames, including one encoding a protein analogous to gp37 of mycobacteriophage L5 and a second encoding a protein with significant homology to the S. coelicolor DNA polymerase .beta. subunit. Based on the DNA sequence analysis, cloning sites can be identified for insertion of reporter genes, making DS6A useful as a reporter phage for specific detection and identification of species of the TB complex.

Abstract: Mycobacteriophage DS6A has been characterized and found to specifically infect all species of the TB complex, without any detectable infection of mycobacteria species other than those of the TB complex. DNA sequence analysis revealed several potential open reading frames, including one encoding a protein analogous to gp37 of mycobacteriophage L5 and a second encoding a protein with significant homology to the S. coelicolor DNA polymerase .beta. subunit. Based on the DNA sequence analysis, cloning sites can be identified for insertion of reporter genes, making DS6A useful as a reporter phage for specific detection and identification of species of the TB complex.

Abstract: Methods for processing samples which may contain mycobacteria which are compatible with both conventional culturing techniques and nucleic acid analysis. It has been found that the harsh chemical treatment previously thought necessary to obtain efficient lysis can be eliminated without loss of lysis efficiency, thereby eliminating reagents which may inhibit subsequent nucleic acid-based reactions. Heat alone is sufficient to lyse mycobacteria, even in buffers which do not contain detergents, chelators, enzymes, etc. Residual reagents introduced by conventional sample processing methods for culture can be successfully removed from the processed pellet without significant loss of microorganisms by at least two washings with saline, water, or buffers compatible with nucleic acid analysis and appropriate centrifugation.

Abstract: A steering assembly (10) for use in turning steerable vehicle wheels includes a rack (12) and a pinion (14). A yoke (28) is continuously pressed against the rack (12) by a yoke spring (32). The yoke (28) has a stem (62) which extends through an opening (64) in a housing (16). During construction of the steering assembly (10), a desired range of transverse movement between the rack (112) and the pinion (14) is obtained by applying force (114) to the rack to position the rack at a first end of the range of transverse movement. The direction of the force applied to the rack (12) is then reversed and the rack and yoke (28) are moved together away from the reference position until the yoke stem (62) has moved through the desired range of transverse movement. A yoke plug (20) is then fixed against movement relative to a main housing section (18). After the rack and pinion steering assembly (10) has been constructed, it may be desired to check the range of transverse movement between the rack (12) and pinion (14).

Abstract: A system for sterilizing an object including a hollow cassette for containing the object, a sealable opening in the cassette for ingress and egress of the object, a seal for forming a fluid-tight seal around the opening, input and output ports in the cassette for receiving and exhausting a sterilizing fluid and a sealing check valve in the input and output ports for providing a fluid-tight seal when no connections are made to the input and output ports such that when the object is sterilized within the cassette, the cassette will maintain a sterilized atmosphere for the object until the cassette is opened to allow use of the object.

Abstract: Genus- and species-specific oligonucleotide probes derived from the M. paratuberculosis 70 kD heat shock protein gene sequence. The probes are useful for detecting Mycobacteria and for identifying specific species of Mycobacteria.

Abstract: Mycobacteriophage DS6A has been characterized and found to specifically infect all species of the TB complex, without any detectable infection of mycobacteria species other than those of the TB complex. DNA sequence analysis revealed several potential open reading frames, including one encoding a protein analogous to gp37 of mycobacteriophage L5 and a second encoding a protein with significant homology to the S. coelicolor DNA polymerase .beta. subunit. Based on the DNA sequence analysis, cloning sites can be identified for insertion of reporter genes, making DS6A useful as a reporter phage for specific detection and identification of species of the TB complex.

Abstract: Apparatus for tying down a load on a railcar having a floor extending between opposed side sills. At each one of a plurality of intervals spaced along the railcar a belt anchor is recessed beneath the floor, adjacent and inwardly of one of the side sills. A first aperture is provided in the floor, above the belt anchor. A belt router is also recessed beneath the floor, adjacent and inwardly of the opposite side sill and at a point transversely opposite the belt anchor. A second aperture is provided in the floor, above the belt router. A belt winder is mounted on the opposite side sill, in alignment with the belt anchor and the belt router. One end of a belt is fixed to the belt anchor, with the belt's opposite end is passed through the first aperture, over the load, through the second aperture, through the belt router and through a third aperture in the opposite side sill for engagement and tensioning of the belt by the belt winder.

Abstract: Methods employing internal oligonucleotide standards in isothermal nucleic acid amplification reactions to determine the efficacy of the amplification reaction and to quantify pre-amplification target levels.