Abstract: Methods, devices and kit for analyzing a sample comprising 1,5-anhydroglucitol and a first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte.

Abstract: Methods, devices and kit for analyzing a sample comprising 1,5-anhydroglucitol and a first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte.

Abstract: Disclosed are methods and kits for analyzing a sample comprising 1,5-anhydroglucitol and a possible first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte. Also provided are kits including reagents for practicing the claimed methods.

Abstract: The present disclosure relates to improved light-based assays, especially ligand binding assays, in which light is emitted from moieties stimulated by stimulating moieties. There is provided a method of generating and detecting a light signal comprising (a) providing a fluid sample comprising an analyte of interest; (b) contacting said sample with a first reagent comprising a molecular recognition element that binds to said analyte; (c) contacting said sample with a second reagent that (i) binds to said analyte in the presence of said first reagent; or (ii) binds to said first reagent; wherein said second reagent contains a light-generating reporter or chemiexcitation emitter; (d) removing unbound first and second reagents; (e) introducing into said sample a source of singlet oxygen or a chemiexcitation stimulator; and (f) measuring the production of light from said reporter.

Abstract: The present disclosure relates to the detection of analytes in high volumetric flow applications. Particular embodiments relate to the use of fluorescence polarization/anisotropy based for detection of analytes in a flow cell. In one testing format, an analyte of interest is probed with reagents containing fluorescent labeled recognition elements. When present in a sample or portion of a sample, the labeled analyte produces a shift in fluorescence polarization/anisotropy/intensity/lifetime as the output signal following the binding of the recognition elements to the analytes.

Abstract: Assay compositions and methods for detection of analytes that include covalent modification of assay elements, such that they are preserved, destroyed, created, or immobilized. Methods for detecting an analyte in a biological sample. The method includes providing a mixture of a biological sample potentially containing the analyte, and a molecular recognition element physically coupled to a covalent modification agent, wherein the molecular recognition element is capable of specific recognition of the analyte, and exposing the mixture to a first set of reaction conditions, wherein the analyte and molecular recognition element can associate to form a recognition complex. Upon formation of the recognition complex, the method further includes generating by use of the covalent modification agent, a template complex; and exposing the template complex to a second set of reaction conditions, wherein the template complex is amplified to generate a detectable product indicative of the presence of the analyte.

Abstract: In some embodiments, the present disclosure pertains to method of screening a biological sample for a plurality of diseases. In some embodiments, such a method comprises obtaining a biological sample from a subject in need thereof. In some embodiments, the biological sample comprises a plurality of biomarkers. In some embodiments, each of the plurality of biomarkers is specific for at least one disease. In some embodiments, the method comprises contacting the biological sample with a display library of peptides. In some embodiments, each peptide in the library may have a unique amino acid sequence. In some embodiments, each of the peptides is physically linked to a nucleic acid sequence that identifies of encodes the peptide. In some embodiments, at least one of the peptides is capable of binding to at least one of the biomarkers in the biological sample. In some embodiments, the method comprises separating the bound peptide particles from the unbound peptide particle.

Abstract: Methods, devices and kit for analyzing a sample comprising 1,5-anhydroglucitol and a first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte.

Abstract: Armor components having a ceramic substrate, a thermal sprayed barrier coating covering the substrate material to form a barrier coated substrate, and an outermost encapsulation of metal generally surrounding at least the periphery of the barrier coated substrate are disclosed herein. The encapsulation of metal was cast to the ceramic substrate as molten metal, and the thermal sprayed barrier coating comprises a cermet material, a ceramic material, or a combination thereof. The ceramic substrate is preferably a ceramic tile for ballistic armor. Also disclosed are armor components having a plurality of the ceramic tiles interconnected by the encapsulation of metal, with the metal, which was casted thereto, surrounding at least the periphery of each of the plurality of the armor components.

Abstract: A durable ceramic and metallic coating has been applied to ceramic tiles to protect the tiles while undergoing a molten metal casting operation. The plasma sprayed coating consists of a ceramic top coat layer of aluminum oxide, zirconium oxide, or other oxides with or without a metallic bond coat layer and with or without a functionally gradient coating. This coating protects the underlying ceramic tile, which is composed of boron carbide, silicon carbide, alumina (Al2O3) or other type of hard ceramic, from reacting chemically with the molten metal. The molten metal is cast around the ceramic tiles to create a lattice of ceramic tiles that are used for protection from projectiles and shrapnel.

Abstract: Selecting which sub-sequences in a database of nucleic acid such as 16S rRNA are highly characteristic of particular groupings of bacteria, microorganisms, fungi, etc. on a substantially phylogenetic tree. Also applicable to viruses comprising viral genomic RNA or DNA. A catalogue of highly characteristic sequences identified by this method is assembled to establish the genetic identity of an unknown organism. The characteristic sequences are used to design nucleic acid hybridization probes that include the characteristic sequence or its complement, or are derived from one or more characteristic sequences. A plurality of these characteristic sequences is used in hybridization to determine the phylogenetic tree position of the organism(s) in a sample. Those target organisms represented in the original sequence database and sufficient characteristic sequences can identify to the species or subspecies level. Oligonucleotide arrays of many probes are especially preferred.

Abstract: A durable ceramic and metallic coating has been applied to ceramic tiles to protect the tiles while undergoing a molten metal casting operation. The plasma sprayed coating consists of a ceramic top coat layer of aluminum oxide, zirconium oxide, or other oxides with or without a metallic bond coat layer and with or without a functionally gradient coating. This coating protects the underlying ceramic tile, which is composed of boron carbide, silicon carbide, alumina (Al2O3) or other type of hard ceramic, from reacting chemically with the molten metal. The molten metal is cast around the ceramic tiles to create a lattice of ceramic tiles that are used for protection from projectiles and shrapnel.

Abstract: A method is disclosed for applying a vibration-damping surface to an article. The method includes providing a coating material comprising a ceramic, metallic or cermet material and a viscoelastic glass frit and plasma spraying the coating material onto an article. The coating material forms a plurality of ceramic, metallic or cermet microstructures having voids with the viscoelastic glass frit distributed to interact with the voids to provide vibration damping. Also disclosed are plasma spray coatings for damping vibrations that includes a ceramic-glass frit composite coating capable of reducing resonant vibrations in a substrate at temperatures between 700° F. to 1500° F. and said plasma spray coating as a coating on a substrate.

Abstract: Labels and methods of producing labels for use in clinical, analytical and pharmaceutical development assays are provided. Labels may comprise shape-encoded particles which may be coupled to ligands such as DNA, RNA and antibodies, where different shapes are used to identify which ligand(s) are present. Labels may also comprise reflectors, including retroreflectors and retroreflectors susceptible to analyte-dependent assembly for efficient homogeneous assays.

Abstract: Labels and methods of producing labels for use in clinical, analytical and pharmaceutical development assays are provided. Labels may comprise shape-encoded particles which may be coupled to ligands such as DNA, RNA and antibodies, where different shapes are used to identify which ligand(s) are present. Labels may also comprise reflectors, including retroreflectors and retroreflectors susceptible to analyte-dependent assembly for efficient homogeneous assays.

Abstract: Labels and methods of producing labels for use in clinical, analytical and pharmaceutical development assays are provided. Labels may comprise shape-encoded particles which may be coupled to ligands such as DNA, RNA and antibodies, where different shapes are used to identify which ligand(s) are present. Labels may also comprise reflectors, including retroreflectors and retroreflectors susceptible to analyte-dependent assembly for efficient homogeneous assays.

Abstract: Selecting which sub-sequences in a database of nucleic acid such as 16S rRNA are highly characteristic of particular groupings of bacteria, microorganisms, fungi, etc. on a substantially phylogenetic tree. Also applicable to viruses comprising viral genomic RNA or DNA. A catalogue of highly characteristic sequences identified by this method is assembled to establish the genetic identity of an unknown organism. The characteristic sequences are used to design nucleic acid hybridization probes that include the characteristic sequence or its complement, or are derived from one or more characteristic sequences. A plurality of these characteristic sequences is used in hybridization to determine the phylogenetic tree position of the organism(s) in a sample. Those target organisms represented in the original sequence database and sufficient characteristic sequences can identify to the species or subspecies level. Oligonucleotide arrays of many probes are especially preferred.

Abstract: A method is disclosed for applying a vibration-damping surface to an article. The method includes providing a coating material comprising a ceramic, metallic or cermet material and a viscoelastic glass frit and plasma spraying the coating material onto an article. The coating material forms a plurality of ceramic, metallic or cermet microstructures having voids with the viscoelastic glass frit distributed to interact with the voids to provide vibration damping. Also disclosed are plasma spray coatings for damping vibrations that includes a ceramic-glass frit composite coating capable of reducing resonant vibrations in a substrate at temperatures between 700° F. to 1500° F. and said plasma spray coating as a coating on a substrate.

Abstract: A methodology for bioassays and diagnostics in which a particulate label (ranging in size from nm-scale molecular assemblages to organisms on the scale of tens or hundreds of microns), such as, but not limited to, nanoparticles, bacteria, bacteriophage, Daphnia, and magnetic particles, serve carriers for analytes bound by molecular recognition elements such as antibodies, aptamers, etc. The described methodology is generally applicable to most pathogen assays and molecular diagnostics and also leads to enhanced sensitivity and convenience of use.

Abstract: An immobilized metal affinity chromatography (IMAC) method for separating and/or purifying compounds containing a non-shielded purine or pyrimidine moiety or group such as nucleic acid, presumably through interaction with the abundant aromatic nitrogen atoms in the purine or pyrimidine moiety. The method can also be used to purify compounds containing purine or pyrimidine moieties where the purine and pyrimidine moieties are shielded from interaction with the column matrix from compounds containing a non-shielded purine or pyrimidine moiety or group. Thus, double-stranded plasmid and genomic DNA, which has no low binding affinity can be easily separated from RNA and/or oligonucleotides which bind strongly to metal-charged chelating matrices. IMAC columns clarify plasmid DNA from bacterial alkaline lysates, purify a ribozyme, and remove primers and other contaminants from PCR reactions. The metal ion affinity of yeast RNA decreases in the order: copper (II), nickel (II), zinc (II), and cobalt (II).