Patents by Inventor Caroline Kreutzer
Caroline Kreutzer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9150827Abstract: A method for producing an L-amino acid comprising: a) cultivating in a medium containing glycerol a recombinant coryneform bacteria which produces the desired L-amino acid and which expresses at least one heterologous polynucleotide of glycerol metabolism, such as glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, gipT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and/or talC, and b) isolating the desired L-amino acid. Preferably, the pathways producing the desired L-amino acid in the coryneform bacteria are amplified.Type: GrantFiled: September 27, 2006Date of Patent: October 6, 2015Assignee: EVONIK DEGUSSA GmbHInventors: Volker F. Wendisch, Doris Rittmann, Hermann Sahm, Caroline Kreutzer
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Patent number: 8592187Abstract: The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.Type: GrantFiled: February 15, 2011Date of Patent: November 26, 2013Assignee: Evonik Degussa GmbHInventors: Brigitte Bathe, Wilfried Claes, Silke Jerrentrup, Caroline Kreutzer, Georg Thierbach
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Publication number: 20130142937Abstract: The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.Type: ApplicationFiled: February 15, 2011Publication date: June 6, 2013Inventors: Brigitte Bathe, Wilfried Claes, Silke Jerrentrup, Caroline Kreutzer, Georg Thierbach
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Patent number: 8293514Abstract: An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.Type: GrantFiled: May 17, 2010Date of Patent: October 23, 2012Assignee: Evonik Degussa GmbHInventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
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Patent number: 8080395Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.Type: GrantFiled: January 19, 2011Date of Patent: December 20, 2011Assignee: Evonik Degussa GmbHInventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
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Publication number: 20110171695Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.Type: ApplicationFiled: January 19, 2011Publication date: July 14, 2011Applicant: EVONIK DEGUSSA GmbHInventors: Brigitte BATHE, Caroline Kreutzer, Georg Thierbach
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Patent number: 7910715Abstract: The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.Type: GrantFiled: November 9, 2007Date of Patent: March 22, 2011Assignee: Evonik Degussa GmbHInventors: Brigitte Bathe, Wilfried Claes, Silke Jerrentrup, Caroline Kreutzer, Georg Thierbach
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Patent number: 7893231Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.Type: GrantFiled: October 25, 2007Date of Patent: February 22, 2011Assignee: Evonik Degussa GmbHInventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
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Publication number: 20100311147Abstract: An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.Type: ApplicationFiled: May 17, 2010Publication date: December 9, 2010Applicant: EVONIK DEGUSSA GMBHInventors: BRIGITTE BATHE, CAROLINE KREUTZER, GEORG THIERBACH
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Publication number: 20100261233Abstract: L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.Type: ApplicationFiled: October 10, 2008Publication date: October 14, 2010Applicant: Evonik Degussa GmbHInventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
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Publication number: 20100255544Abstract: The invention relates to coryneform bacteria which, instead of the singular copy of an open reading frame (ORF), gene or allele naturally present at the particular desired site (locus), have at least two copies of the open reading frame (ORF), gene or allele in question, preferably in tandem arrangement, and optionally at least a third copy of the open reading frame (ORF), gene or allele in question at a further gene site, and processes for the preparation of chemical compounds by fermentation of these bacteria.Type: ApplicationFiled: September 3, 2009Publication date: October 7, 2010Applicant: Evonik Degussa GmbHInventors: Brigitte Bathe, Caroline Kreutzer, Bettina Mockel, Georg Thierbach
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Patent number: 7754446Abstract: An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.Type: GrantFiled: October 16, 2007Date of Patent: July 13, 2010Assignee: Evonik Degussa GmbHInventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
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Publication number: 20100159523Abstract: The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.Type: ApplicationFiled: September 23, 2009Publication date: June 24, 2010Applicant: EVONIK DEGUSSA GMBHInventors: BRIGITTE BATHE, CAROLINE KREUTZER, BETTINA MOCKEL, GEORG THIERBACH
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Publication number: 20090053794Abstract: An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.Type: ApplicationFiled: October 16, 2007Publication date: February 26, 2009Applicant: EVONIK DEGUSSA GmbHInventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
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Publication number: 20080293100Abstract: The invention relates to a method for producing L-amino acids in which the following steps are carried out: a) the recombinant coryneform bacteria which produce the desired L-amino acid and in which at least one or several of the heterologous polynucleotides of the glycerol metabolism, selected among the group comprising glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, glpT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and talC, is/are expressed are cultivated in a medium containing glycerol or one or several other optional C sources in conditions in which the desired L-amino acid is enriched in the medium or the cells; and, optionally, b) the desired L-amino acid is isolated, all or fractions (>0 to 100 percent) of the components of the fermentation broth and/or biomass optionally remaining in the final product.Type: ApplicationFiled: September 27, 2006Publication date: November 27, 2008Applicant: Degussa GmbHInventors: Volker F. Wendisch, Doris Rittmann, Hermann Sahm, Caroline Kreutzer
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Publication number: 20080274265Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.Type: ApplicationFiled: October 25, 2007Publication date: November 6, 2008Applicant: EVONIK DEGUSSA GmbHInventors: Brigitte BATHE, Caroline Kreutzer, Georg Thierbach
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Publication number: 20080261269Abstract: The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.Type: ApplicationFiled: November 9, 2007Publication date: October 23, 2008Applicant: EVONIK DEGUSSA GMBHInventors: Brigitte Bathe, Wilfried Claes, Silke Jerrentrup, Caroline Kreutzer, Georg Thierbach
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Patent number: 7416863Abstract: Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.Type: GrantFiled: July 12, 2005Date of Patent: August 26, 2008Assignee: Degussa AGInventors: Bettina Moeckel, Mike Farwick, Thomas Hermann, Caroline Kreutzer, Walter Pfefferle
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Publication number: 20070111291Abstract: The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.Type: ApplicationFiled: December 18, 2006Publication date: May 17, 2007Inventors: Brigitte Bathe, Caroline Kreutzer, Bettina Mockel, Georg Thierbach
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Publication number: 20070054380Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.Type: ApplicationFiled: June 19, 2006Publication date: March 8, 2007Inventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek