Patents by Inventor Caroline Kreutzer

Caroline Kreutzer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9150827
    Abstract: A method for producing an L-amino acid comprising: a) cultivating in a medium containing glycerol a recombinant coryneform bacteria which produces the desired L-amino acid and which expresses at least one heterologous polynucleotide of glycerol metabolism, such as glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, gipT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and/or talC, and b) isolating the desired L-amino acid. Preferably, the pathways producing the desired L-amino acid in the coryneform bacteria are amplified.
    Type: Grant
    Filed: September 27, 2006
    Date of Patent: October 6, 2015
    Assignee: EVONIK DEGUSSA GmbH
    Inventors: Volker F. Wendisch, Doris Rittmann, Hermann Sahm, Caroline Kreutzer
  • Patent number: 8592187
    Abstract: The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.
    Type: Grant
    Filed: February 15, 2011
    Date of Patent: November 26, 2013
    Assignee: Evonik Degussa GmbH
    Inventors: Brigitte Bathe, Wilfried Claes, Silke Jerrentrup, Caroline Kreutzer, Georg Thierbach
  • Publication number: 20130142937
    Abstract: The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.
    Type: Application
    Filed: February 15, 2011
    Publication date: June 6, 2013
    Inventors: Brigitte Bathe, Wilfried Claes, Silke Jerrentrup, Caroline Kreutzer, Georg Thierbach
  • Patent number: 8293514
    Abstract: An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.
    Type: Grant
    Filed: May 17, 2010
    Date of Patent: October 23, 2012
    Assignee: Evonik Degussa GmbH
    Inventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
  • Patent number: 8080395
    Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.
    Type: Grant
    Filed: January 19, 2011
    Date of Patent: December 20, 2011
    Assignee: Evonik Degussa GmbH
    Inventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
  • Publication number: 20110171695
    Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.
    Type: Application
    Filed: January 19, 2011
    Publication date: July 14, 2011
    Applicant: EVONIK DEGUSSA GmbH
    Inventors: Brigitte BATHE, Caroline Kreutzer, Georg Thierbach
  • Patent number: 7910715
    Abstract: The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.
    Type: Grant
    Filed: November 9, 2007
    Date of Patent: March 22, 2011
    Assignee: Evonik Degussa GmbH
    Inventors: Brigitte Bathe, Wilfried Claes, Silke Jerrentrup, Caroline Kreutzer, Georg Thierbach
  • Patent number: 7893231
    Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.
    Type: Grant
    Filed: October 25, 2007
    Date of Patent: February 22, 2011
    Assignee: Evonik Degussa GmbH
    Inventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
  • Publication number: 20100311147
    Abstract: An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.
    Type: Application
    Filed: May 17, 2010
    Publication date: December 9, 2010
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: BRIGITTE BATHE, CAROLINE KREUTZER, GEORG THIERBACH
  • Publication number: 20100261233
    Abstract: L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
    Type: Application
    Filed: October 10, 2008
    Publication date: October 14, 2010
    Applicant: Evonik Degussa GmbH
    Inventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
  • Publication number: 20100255544
    Abstract: The invention relates to coryneform bacteria which, instead of the singular copy of an open reading frame (ORF), gene or allele naturally present at the particular desired site (locus), have at least two copies of the open reading frame (ORF), gene or allele in question, preferably in tandem arrangement, and optionally at least a third copy of the open reading frame (ORF), gene or allele in question at a further gene site, and processes for the preparation of chemical compounds by fermentation of these bacteria.
    Type: Application
    Filed: September 3, 2009
    Publication date: October 7, 2010
    Applicant: Evonik Degussa GmbH
    Inventors: Brigitte Bathe, Caroline Kreutzer, Bettina Mockel, Georg Thierbach
  • Patent number: 7754446
    Abstract: An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.
    Type: Grant
    Filed: October 16, 2007
    Date of Patent: July 13, 2010
    Assignee: Evonik Degussa GmbH
    Inventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
  • Publication number: 20100159523
    Abstract: The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.
    Type: Application
    Filed: September 23, 2009
    Publication date: June 24, 2010
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: BRIGITTE BATHE, CAROLINE KREUTZER, BETTINA MOCKEL, GEORG THIERBACH
  • Publication number: 20090053794
    Abstract: An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.
    Type: Application
    Filed: October 16, 2007
    Publication date: February 26, 2009
    Applicant: EVONIK DEGUSSA GmbH
    Inventors: Brigitte Bathe, Caroline Kreutzer, Georg Thierbach
  • Publication number: 20080293100
    Abstract: The invention relates to a method for producing L-amino acids in which the following steps are carried out: a) the recombinant coryneform bacteria which produce the desired L-amino acid and in which at least one or several of the heterologous polynucleotides of the glycerol metabolism, selected among the group comprising glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, glpT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and talC, is/are expressed are cultivated in a medium containing glycerol or one or several other optional C sources in conditions in which the desired L-amino acid is enriched in the medium or the cells; and, optionally, b) the desired L-amino acid is isolated, all or fractions (>0 to 100 percent) of the components of the fermentation broth and/or biomass optionally remaining in the final product.
    Type: Application
    Filed: September 27, 2006
    Publication date: November 27, 2008
    Applicant: Degussa GmbH
    Inventors: Volker F. Wendisch, Doris Rittmann, Hermann Sahm, Caroline Kreutzer
  • Publication number: 20080274265
    Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.
    Type: Application
    Filed: October 25, 2007
    Publication date: November 6, 2008
    Applicant: EVONIK DEGUSSA GmbH
    Inventors: Brigitte BATHE, Caroline Kreutzer, Georg Thierbach
  • Publication number: 20080261269
    Abstract: The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.
    Type: Application
    Filed: November 9, 2007
    Publication date: October 23, 2008
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: Brigitte Bathe, Wilfried Claes, Silke Jerrentrup, Caroline Kreutzer, Georg Thierbach
  • Patent number: 7416863
    Abstract: Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.
    Type: Grant
    Filed: July 12, 2005
    Date of Patent: August 26, 2008
    Assignee: Degussa AG
    Inventors: Bettina Moeckel, Mike Farwick, Thomas Hermann, Caroline Kreutzer, Walter Pfefferle
  • Publication number: 20070111291
    Abstract: The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.
    Type: Application
    Filed: December 18, 2006
    Publication date: May 17, 2007
    Inventors: Brigitte Bathe, Caroline Kreutzer, Bettina Mockel, Georg Thierbach
  • Publication number: 20070054380
    Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
    Type: Application
    Filed: June 19, 2006
    Publication date: March 8, 2007
    Inventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek