Patents by Inventor Carolyn Bertozzi
Carolyn Bertozzi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20060063736Abstract: The present invention provides inhibitors of mucin-type O-linked glycosylation, and in particular inhibitors of polypeptide N-acetyl-?-galactosaminyltransferases; as well as compositions comprising the inhibitors. The present invention further provides methods of identifying inhibitors of polypeptide N-acetyl-?-galactosaminyltransferases. The inhibitors are useful in various applications, including research applications, and treatment methods.Type: ApplicationFiled: March 23, 2005Publication date: March 23, 2006Inventors: Carolyn Bertozzi, Howard Hang
-
Publication number: 20060035305Abstract: The invention provides methods and compositions useful for making synthetic peptide conjugates. In one embodiment, the invention provides compositions comprising the structure: wherein R is selected from lower substituted or unsubstituted alkyl, O, NH and S and P is an amine protection group. In more particular embodiments, the compositions comprise ?-amine protected 4,5-dehydroleucine or ?-amine protected (2S)-aminolevulinic acid and/or P is F-moc. These compounds may be incorporated into peptides, for example, peptides comprising a substituted or unsubstituted (2S)-aminolevulinic acid residue, such as (2S)-aminolevulinic acid residue is substituted with an O- or N-linked glycoconjugate, or a detectable label.Type: ApplicationFiled: September 6, 2005Publication date: February 16, 2006Inventor: Carolyn Bertozzi
-
Publication number: 20060035389Abstract: Methods and compositions for the immobilization of glycoproteins are presented herein. In addition, the present invention provides arrays of immobilized glycoproteins. The methods of immobilizing glycoproteins include oxidation of the glycosyl moiety.Type: ApplicationFiled: May 15, 2003Publication date: February 16, 2006Inventors: Paul Peluso, Carolyn Bertozzi
-
Publication number: 20050282271Abstract: Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.Type: ApplicationFiled: July 25, 2005Publication date: December 22, 2005Inventors: Carolyn Bertozzi, Kevin Yarema, Lara Mahal
-
Patent number: 6936701Abstract: Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.Type: GrantFiled: September 24, 2002Date of Patent: August 30, 2005Assignee: The Regents of the University of CaliforniaInventors: Carolyn Bertozzi, Kevin J. Yarema, Lara K. Mahal
-
Publication number: 20050148032Abstract: The present invention features a chemoselective ligation reaction that can be carried out under physiological conditions. In general, the invention involves condensation of a specifically engineered phosphine, which can provide for formation of an amide bond between the two reactive partners resulting in a final product comprising a phosphine moiety, or which can be engineered to comprise a cleavable linker so that a substituent of the phosphine is transferred to the azide, releasing an oxidized phosphine byproduct and producing a native amide bond in the final product. The selectivity of the reaction and its compatibility with aqueous environments provides for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).Type: ApplicationFiled: January 19, 2005Publication date: July 7, 2005Inventors: Eliana Saxon, Carolyn Bertozzi
-
Patent number: 6713274Abstract: The instant invention provides methods for identifying agents that modulate an enzymatic activity of a carbohydrate sulfotransferase. The methods generally involve contacting the sulfotransferase, in a reaction solution, with a sulfate donor, a test agent, and a polymeric sulfate acceptor that is readily separated from the reaction solution. Determination of an effect of the test agent on the sulfotransferase is by detecting the amount of sulfate in the polymeric sulfate acceptor that has been separated from the reaction solution. The invention further provides kits for use in carrying out the subject methods.Type: GrantFiled: May 13, 2002Date of Patent: March 30, 2004Assignee: The Regents of the University of CaliforniaInventors: Carolyn Bertozzi, Dawn Verdugo
-
Publication number: 20030211562Abstract: The instant invention provides methods for identifying agents that modulate an enzymatic activity of a carbohydrate sulfotransferase. The methods generally involve contacting the sulfotransferase, in a reaction solution, with a sulfate donor, a test agent, and a polymeric sulfate acceptor that is readily separated from the reaction solution. Determination of an effect of the test agent on the sulfotransferase is by detecting the amount of sulfate in the polymeric sulfate acceptor that has been separated from the reaction solution. The invention further provides kits for use in carrying out the subject methods.Type: ApplicationFiled: May 13, 2002Publication date: November 13, 2003Inventors: Carolyn Bertozzi, Dawn Verdugo
-
Patent number: 6570040Abstract: The present invention features a chemoselective ligation reaction that can be carried out under physiological conditions. In general, the invention involves condensation of a specifically engineered phosphine, which can provide for formation of an amide bond between the two reactive partners resulting in a final product comprising a phosphine moiety, or which can be engineered to comprise a cleavable linker so that a substituent of the phosphine is transferred to the azide, releasing an oxidized phosphine byproduct and producing a native amide bond in the final product. The selectivity of the reaction and its compatibility with aqueous environments provides for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).Type: GrantFiled: March 16, 2001Date of Patent: May 27, 2003Assignee: The Regents of the University of CaliforniaInventors: Eliana Saxon, Carolyn Bertozzi
-
Patent number: 6552103Abstract: Novel biomimetic hydrogel materials and methods for their preparation. Hydrogels containing acrylamide-functionalized carbohydrate, sulfoxide, sulfide or sulfone copolymerized with a hydrophilic or hydrophobic copolymerizing material selected from the group consisting of an acrylamide, methacrylamide, acrylate, methacrylate, vinyl and a derivative thereof present in concentration from about 1 to about 99 wt %. and methods for their preparation. The method of use of the new hydrogels for fabrication of soft contact lenses and biomedical implants.Type: GrantFiled: May 19, 2000Date of Patent: April 22, 2003Assignee: The Regents of the University of CaliforniaInventors: Carolyn Bertozzi, Ravindranath Mukkamala, Oing Chen, Hopin Hu, Dominique Baude
-
Patent number: 6458937Abstract: Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.Type: GrantFiled: May 16, 2000Date of Patent: October 1, 2002Assignee: The Regents of the University of CaliforniaInventors: Carolyn Bertozzi, Kevin J. Yarema, Lara K. Mahal
-
Publication number: 20010046970Abstract: This invention provides compositions for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 106 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, these composition scan be used to palliate certain inflammatory and immunological conditions.Type: ApplicationFiled: June 22, 2001Publication date: November 29, 2001Inventors: Jon O. Nagy, Wayne R. Spevak, Falguni Dasgupta, Carolyn Bertozzi
-
Patent number: 6107365Abstract: Novel biomimetic hydrogel materials and methods for their preparation. Hydrogels containing acrylamide-functionalized carbohydrate, sulfoxide, sulfide or sulfone copolymerized with a hydrophilic or hydrophobic copolymerizing material selected from the group consisting of an acrylamide, methacrylamide, acrylate, methacrylate, vinyl and a derivative thereof present in concentration from about 1 to about 99 wt %. and methods for their preparation. The method of use of the new hydrogels for fabrication of soft contact lenses and biomedical implants.Type: GrantFiled: September 2, 1998Date of Patent: August 22, 2000Assignee: The Regents of the University of CaliforniaInventors: Carolyn Bertozzi, Ravindranath Mukkamala, Qing Chen, Hopin Hu, Dominique Baude
-
Patent number: 6075134Abstract: Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.Type: GrantFiled: May 15, 1997Date of Patent: June 13, 2000Assignee: The Regents of the University of CaliforniaInventors: Carolyn Bertozzi, Kevin J. Yarema, Lara K. Mahal
-
Patent number: 5977080Abstract: Sulfated disaccharides characterized by the ability to inhibit the binding of selectin to its physiologically-relevant ligand are disclosed. Included are efficient and inexpensive methods for producing the sulfated disaccharides, and methods for their therapeutic use and in in vivo and in vitro assays.Type: GrantFiled: January 8, 1998Date of Patent: November 2, 1999Assignee: The Regents Of The University Of CaliforniaInventors: Steven D. Rosen, Carolyn Bertozzi
-
Patent number: 5962422Abstract: This invention provides a system for inhibiting the binding between two cells, one expressing P- or L-selectin on the surface and the other expressing the corresponding ligand. A covalently crosslinked lipid composition is prepared having saccharides and acidic group on separate lipids. The composition is then interposed between the cells so as to inhibit binding. Inhibition can be achieved at an effective oligosaccharide concentration as low as 10.sup.6 fold below that of the free saccharide. Since selectins are involved in recruiting cells to sites of injury, this system can be used to palliate certain inflammatory and immunological conditions.Type: GrantFiled: February 28, 1997Date of Patent: October 5, 1999Assignee: The Regents of the University of CaliforniaInventors: Jon O. Nagy, Wayne R. Spevak, Falguni Dasgupta, Carolyn Bertozzi
-
Patent number: 5783693Abstract: Sulfated disaccharides characterized by the ability to inhibit the binding of selectin to its physiologically-relevant ligand are disclosed. Included are efficient and inexpensive methods for producing the sulfated disaccharides, and methods for their therapeutic use and in in vivo and in vitro assays.Type: GrantFiled: August 23, 1995Date of Patent: July 21, 1998Assignee: The Regents of the University of CaliforniaInventors: Carolyn Bertozzi, Steven D. Rosen