Patents by Inventor Charles K. Brush

Charles K. Brush has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8378115
    Abstract: Monomethine dyes that have no or minimal fluorescence in buffer or in the presence of single stranded DNA or RNA, but strongly fluoresce in the presence of double-stranded DNA. In one embodiment, the dye is useful in quantitative RT-PCR.
    Type: Grant
    Filed: September 30, 2008
    Date of Patent: February 19, 2013
    Assignee: Thermo Fisher Scientific (Milwaukee) LLC
    Inventors: Charles K. Brush, Jianqin Liu, Peter Czerney, Matthias Wenzel
  • Patent number: 7951959
    Abstract: Compounds, compositions, and methods for optical, including fluorescence optical, determinations useful in labeling biomolecules such as protein and deoxyribonucleic acid for their detection and quantitation. The compounds are diastereomeric cyanines with high hydrophilicity and other desirable properties.
    Type: Grant
    Filed: September 16, 2009
    Date of Patent: May 31, 2011
    Assignee: Thermo Fisher Scientific (Milwaukee) LLC
    Inventors: Charles K. Brush, Kaizhang He, Peter T. Czerney, Matthias Wenzel
  • Publication number: 20100285470
    Abstract: Monomethine dyes that have no or minimal fluorescence in buffer or in the presence of single stranded DNA or RNA, but strongly fluoresce in the presence of double-stranded DNA. In one embodiment, the dye is useful in quantitative RT-PCR.
    Type: Application
    Filed: September 30, 2008
    Publication date: November 11, 2010
    Applicant: Thermo Fisher Scientific (Milwaukee) LLC
    Inventors: Charles K. Brush, Jianqin Liu, Peter Czerney, Matthias Wenzel
  • Publication number: 20100069258
    Abstract: Compounds, compositions, and methods for optical, including fluorescence optical, determinations useful in labeling biomolecules such as protein and deoxyribonucleic acid for their detection and quantitation. The compounds are diastereomeric cyanines with high hydrophilicity and other desirable properties.
    Type: Application
    Filed: September 16, 2009
    Publication date: March 18, 2010
    Applicant: Thermo Fisher Scientific (Milwaukee) LLC
    Inventors: Charles K. Brush, Kaizhang He, Peter T. Czemey, Matthias Wenzel
  • Publication number: 20090048436
    Abstract: The present invention provides a method of synthesizing phosphoramidite linkers that are useful for the production of synthesizing two or more oligonucleotides in tandem. The inventive linker has the following desirable properties: (i) enhanced stability to alkali conditions versus the linkers previously published, (ii) cleaves to produce 5? and 3? ends that are fully biologically compatible, (iii) cleaves completely under conditions that are already used in cleavage/deprotection processes so it is fully compatible with conditions that are common in laboratories and does not require additives that necessitate further purification after cleavage, (iv) integrates easily onto commercially available synthesizers because it is compatible with standard coupling chemistry, and (v) is compatible with DNA, RNA, forward, reverse, and LNA, synthesis chemistries. In addition, the inventive linkers may be coupled to a solid support. Thus, the inventive linkers provide a significant advancement in the state of the art.
    Type: Application
    Filed: August 15, 2007
    Publication date: February 19, 2009
    Inventors: Keith Anderson, Michael Jensen, Ronald W. Davis, Charles K. Brush, Kaizhang He
  • Patent number: 7223538
    Abstract: An assay is provided for nucleic acids that can be post-synthetically labeled, wherein modified nucleoside triphosphates are used that are more efficiently and specifically incorporated during nucleic acid synthesis than labeled nucleoside triphosphates. In a preferred embodiment, nucleoside ?-thiotriphosphates are utilized. Maleimide or iodoacetamide conjugating moieties can be attached post-synthetically. The conjugating moieties may include a reporter group. Also disclosed are new methods for detecting single nucleotide polymorphism.
    Type: Grant
    Filed: December 14, 2001
    Date of Patent: May 29, 2007
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Charles K. Brush, Vineet Gupta
  • Patent number: 6921638
    Abstract: Methods and devices for detecting nucleic acid and protein targets on hydrogel microarrays are disclosed. Fluorophores are incorporated into the targets and detected. A linear correlation between target concentration and signal amplitude is maintained through the elimination of active enzyme amplification.
    Type: Grant
    Filed: December 19, 2001
    Date of Patent: July 26, 2005
    Assignee: Amersham Biosciences AB
    Inventors: Chang-Gong Liu, Abhijit Mazumder, Charles K. Brush, W. Travis Johnson
  • Publication number: 20040014078
    Abstract: This invention is directed to novel methods of amplifying and detecting DNA. More specifically, the invention applies variations of Rolling Circle Amplification to several detection platforms.
    Type: Application
    Filed: February 6, 2003
    Publication date: January 22, 2004
    Inventors: James Xia, Charles K. Brush, Vineet Gupta, Heshu Huang, Changming Li, George Maracas, Robert Marrero, Melissa L. Ray, Lei Sun, Peiming Zhang
  • Patent number: 6664061
    Abstract: [2+2] photo-attachable functional groups were incorporated in polyacrylamide based hydrogels and synthetic oligonucleotide probes. The probes were photochemically attached by covalent bonding to the three dimensional surface of a hydrogel.
    Type: Grant
    Filed: August 9, 2001
    Date of Patent: December 16, 2003
    Assignee: Amersham Biosciences AB
    Inventors: Robert Elghanian, Charles K. Brush, Yanzheng Xu
  • Publication number: 20030124525
    Abstract: The present invention provides solid supports (e.g., glass) and polymer hydrogels (particularly polymer hydrogel arrays present on a solid support) comprising one or more reactive sites for the attachment of biomolecules, as well as biomolecules comprising one or more reactive sites for attachment to solid supports and polymer hydrogels. The invention further provides novel compositions and methods for the preparation of biomolecules, solid supports, and polymer hydrogels comprising reactive sites. The invention also provides for preparation of crosslinked solid supports, polymer hydrogels, and hydrogel arrays, wherein one or more biomolecules is attached by means of the reactive sites in a photocycloaddition reaction. Advantageously, according to the invention, crosslinking of the hydrogel and attachment of biomolecules can be done in a single step.
    Type: Application
    Filed: August 9, 2001
    Publication date: July 3, 2003
    Inventors: Robert Elghanian, Charles K. Brush, Yanzheng Xu
  • Publication number: 20030119005
    Abstract: An assay is provided for nucleic acids that can be post-synthetically labeled, wherein modified nucleoside triphosphates are used that are more efficiently and specifically incorporated during nucleic acid synthesis than labeled nucleoside triphosphates. In a preferred embodiment, nucleoside &agr;-thiotriphosphates are utilized. Maleimide or iodoacetamide conjugating moieties can be attached post-synthetically. The conjugating moieties may include a reporter group. Also disclosed are new methods for detecting single nucleotide polymorphism.
    Type: Application
    Filed: December 14, 2001
    Publication date: June 26, 2003
    Applicant: MOTOROLA, INC.
    Inventors: Charles K. Brush, Vineet Gupta
  • Publication number: 20030096265
    Abstract: Photoreactive phosphoramidites useful for attaching photoreactive sites to nucleic acids and oligonucleotides are synthesized. The resultant nucleic acid or oligonucleotide probes incorporating the photoreactive sites are then attached to a polymer-coated support by a [2+2] cycloaddition to form a microarray.
    Type: Application
    Filed: June 28, 2002
    Publication date: May 22, 2003
    Applicant: Motorola, Inc.
    Inventors: Charles K. Brush, Robert Elghanian, Yanzheng Xu
  • Publication number: 20020146730
    Abstract: Methods and devices for detecting nucleic acid and protein targets on hydrogel microarrays are disclosed. Fluorophores are incorporated into the targets and detected. A linear correlation between target concentration and signal amplitude is maintained through the elimination of active enzyme amplification.
    Type: Application
    Filed: December 19, 2001
    Publication date: October 10, 2002
    Inventors: Chang-Gong Liu, Abhijit Mazumder, Charles K. Brush, W. Travis Johnson
  • Patent number: 5986086
    Abstract: A chemical compound of the following formula: ##STR1## wherein R.sup.1 is selected from the group consisting of alkyl, aralkyl, and substituted alkyl groups; R.sup.3 is selected from the group consisting of H, PO.sub.3.sup.-2, P.sub.2 O.sub.6.sup.-3 ; P.sub.3 O.sub.9.sup.-4, and .alpha.-thio phoshates (PSO.sub.2.sup.-2 ; P.sub.2 SO.sub.5.sup.-3 ; P.sub.3 O.sub.8.sup.-4); and .alpha.BH.sub.3.sup.- phosphates (P(BH.sub.3)O.sub.2.sup.-2, P.sub.2 (BH.sub.3)O.sub.5.sup.-3, P.sub.3 (BH.sub.3)O.sub.8.sup.-4); R.sup.4 is selected from the group consisting of H, lower alkyl, acyl, (CH.sub.2).sub.p COO(CH.sub.2).sub.q CH.sub.3 wherein p is an integer from 0 to 4 and q is an integer from 0 to 4, and 5,6; 6,7; or 7,8-butadienyl; R.sup.5 is selected from the group consisting of H lower alkyl, acyl, (CH.sub.2).sub.p COO(CH.sub.2).sub.q CH.sub.
    Type: Grant
    Filed: June 20, 1997
    Date of Patent: November 16, 1999
    Assignee: Amersham Pharmacia Biotech Inc.
    Inventors: Charles K. Brush, Ned D. Reimer
  • Patent number: 5808044
    Abstract: A chemical compound of the following formula is disclosed: ##STR1## where R is selected from the group consisting of H, trityl, 4-O-monomethoxytrityl, 4,4'-O-dimethoxytrityl, and acyl groups, and whereby R can be used as a protecting group or is an H;R' is a phosphoramidite;R" is selected from the group consisting of H and lower alkyl groups;R'" is selected from the group consisting of H and lower alkyl groups;R.sup.4 is selected from the group consisting of H, lower alkyl, acyl, ##STR2## and (CH.sub.2).sub.p COO(CH.sub.2).sub.q CH.sub.3 wherein p is an integer from 0 to 4 and q is an integer from 0 to 4;R.sup.5 is selected from the group consisting of H, lower alkyl, acyl, ##STR3## and (CH.sub.2).sub.p COO(CH.sub.2).sub.q CH.sub.3 wherein p is an integer from 0 to 4 and q is an integer from 0 to 4;n is an integer from 0 to 10;m is an integer from 0 to 10;r is 1, 2, or 3; andX.sup.- is a negative ion.
    Type: Grant
    Filed: February 10, 1997
    Date of Patent: September 15, 1998
    Assignee: Pharmacia Biotech Inc.
    Inventors: Charles K. Brush, Eric Dean Anderson
  • Patent number: 5808043
    Abstract: A preparation of a labelled nucleotide comprising at least one compound having a Mg.sup.2+ association constant between 1.times.10.sup.-11 to 1.times.10.sup.-2, inclusive. The compound is preferably selected from the group consisting of citrate, isocitrate, phosphate, EGTA, EDTA, and CDTA. The concentration of the compound is preferably at least 5 mM.
    Type: Grant
    Filed: January 18, 1995
    Date of Patent: September 15, 1998
    Assignee: Pharmacia Biotech Inc.
    Inventors: R. Scott Duthie, Charles K. Brush, Eugene P. Stirchak, Mark E. Freeman, Lawrence J. Burazin
  • Patent number: 5721355
    Abstract: Compounds useful to attach a fluorescein label to an oligonucleotide are disclosed. To create these compounds, oxygen groups on the fluorescein moiety are protected (e.g. with acyl groups). One then links a phosphoramidite to the fluorescein moiety via an amide or thiourea linkage to an active site on the fluorescein ring. The resulting compound can then be directly linked to an oligonucleotide as it is being formed in an automated synthesizer and then deprotected using the same deprotection conditions as are used to deprotect the oligonucleotides. Such compounds are, inter alia, useful to create labelled primers for DNA sequencing.
    Type: Grant
    Filed: June 25, 1996
    Date of Patent: February 24, 1998
    Assignee: Pharmacia Biotech Inc.
    Inventor: Charles K. Brush
  • Patent number: 5583236
    Abstract: Compounds useful to attach a fluorescein label to an oligonucleotide are disclosed. To create these compounds, oxygen groups on the fluorescein moiety are protected (e.g. with acyl groups). One then links a phosphoramidite to the fluorescein moiety via an amide or thiourea linkage to an active site on the fluorescein ring. The resulting compound can then be directly linked to an oligonucleotide as it is being formed in an automated synthesizer and then deprotected using the same deprotection conditions as are used to deprotect the oligonucleotides. Such compounds are, inter alia, useful to create labelled primers for DNA sequencing.
    Type: Grant
    Filed: December 2, 1994
    Date of Patent: December 10, 1996
    Assignee: Pharmacia Biotech Inc.
    Inventor: Charles K. Brush
  • Patent number: 5556959
    Abstract: A chemical compound of the following formula is disclosed ##STR1## where R is one of H, trityl, 4-0-monomethoxytrityl, 4,4'-O-dimethoxytrityl, or acyl groups and R may be used as a protecting group or is an H; R.sup.1 is a phosphoramidite; R.sup.11 is one of H or lower alkyl groups; R.sup.111 is one of H or lower alkyl groups; R.sup.4 is one of H, lower alkyl, acyl, or (CH.sub.2).sub.p COO(CH.sub.2).sub.q CH.sub.3 where p is an integer from 0 to 4 and q is an integer from 0 to 4; R.sup.5 is one of H, lower alkyl, acyl, or (CH.sub.2).sub.p COO(CH.sub.2).sub.q CH.sub.3 where p is an integer from 0 to 4 and q is an integer from 0 to 4; n is an integer from 0 to 10; m is an integer from 0 to 10; and r is 1, 2, or 3.
    Type: Grant
    Filed: June 24, 1994
    Date of Patent: September 17, 1996
    Assignee: Pharmacia P-L Biochemicals Inc.
    Inventors: Charles K. Brush, Eric D. Anderson
  • Patent number: 5420330
    Abstract: Ether variant lipids bound to phosphoramidites are disclosed. These can be used to create oligonucleotides that are linked to stable lipid-like units. For example, dialkyl glycerols are linked to phosphoramidites.
    Type: Grant
    Filed: September 7, 1990
    Date of Patent: May 30, 1995
    Assignee: Pharmacia P-L Biochemicals Inc.
    Inventor: Charles K. Brush