Abstract: Disclosed in the present application is a method for constructing an antigen-specific binding polypeptide gene display vector. The method comprises processing by using a restriction endonuclease that specifically recognizes a restriction site to obtain four nucleic acid fragments having specific sticky ends, and then enabling the nucleic acid fragments to directionally ligate. Further disclosed in the present application are an antigen-specific binding polypeptide gene display vector produced according to the method and a bacterial library. The method described in the present application can be used for effectively screening antigen-specific antigen-binding polypeptides or fragments thereof.

Abstract: The present invention discloses production and processing equipment for oxygen-free copper alloy thick strips and a processing method thereof, relating to the technical field of oxygen-free copper alloy processing. The equipment includes a cooling box and a pickling box. Both the cooling box and the pickling box are internally provided with a transmission mechanism, and the cooling box is internally provided with a relief mechanism for rapidly cooling an oxygen-free copper alloy plate and reducing the thickness of an oxide layer. The cooling box is internally provided with a pumping mechanism, and the pickling box is internally provided with a cleaning mechanism for performing pickling treatment on the oxide layer. The pickling box is internally provided with a polishing mechanism for removing the oxide layer on a surface of the oxygen-free copper alloy plate.

Abstract: The present invention relates to systems and methods for catalytic removal of oxidized contaminants (for example, nitrite, chromate, chlorate, trichloroethene, CFC-11, 4-nonylphenol, RDX, and perfluorooctanoate) from water and wastewater. In some aspects, the catalytic method of removing oxidized contaminants comprises using precious metal nanoparticles as catalysts to reduce the oxidized contaminants.

Abstract: The present invention is directed to systems and methods for removing trichloroethane (TCA), trichloroethene (TCE), and 1,4-dioxane (1,4-D) from contaminated water and wastewater. The system and methods relying on catalyst reduction of TCA and TCE, and the reduced products are degraded by microorganisms that are capable of biodegrading ethane and 1,4-D. In certain embodiments, a catalyst film comprises precious nanoparticles with diameters of 5-40 nm and a biofilm comprising microorganisms that are capable of degrading ethane and 1,4-D are used in a dual-reactor system to remove TCA, TCE, and 1,4-D from contaminated water and wastewater.

Abstract: An integrated cooling source apparatus is connected to an air-to-liquid heat exchange mode component through a liquid cooling pipeline and is connected to a liquid-to-liquid heat exchange mode component through the liquid cooling pipeline. The air-to-liquid heat exchange mode component and the liquid-to-liquid heat exchange mode component are connected through the liquid cooling pipeline. The integrated cooling source apparatus is configured to provide a coolant through the liquid cooling pipeline, and the coolant sequentially passes through the air-to-liquid heat exchange mode component configured to dissipate heat for a first component of a device and the liquid-to-liquid heat exchange mode component configured to dissipate heat for a second component of the device. The integrated cooling source apparatus is further configured to receive the coolant that is output after heat is dissipated for the second component.

Abstract: Provided are a 7-cyano-8-hydroxyquinoline derivative, a preparation method therefor and a medical use thereof specifically, disclosed are a compound represented by general formula (I), a preparation method therefor, a pharmaceutical composition containing same, and a use thereof in the treatment of infectious diseases or cancer. The compound has excellent antibacterial, antiviral and antitumor activity, and can be developed as a drug for the treatment of infectious diseases or tumors. The definitions of groups in the general formula (I) are the same as in the description.

Abstract: The present invention relates to a crystal form of a nitroxoline prodrug, a pharmaceutical composition containing same, and a preparation method therefor and an application thereof. An X-ray powder diffraction pattern of a crystal form A comprises characteristic peaks at 5.74±0.2°, 6.78±0.2°, 10.86±0.2°, 13.54±0.2°, 16.70±0.2°, and 22.65±0.2°; an X-ray powder diffraction pattern of a crystal form B comprises characteristic peaks at 5.44±0.2°, 10.90±0.2°, 14.09±0.2°, 16.17±0.2°, 17.92±0.2°, 20.66±0.2°, and 23.13±0.2°. Compared with nitroxoline, the crystal forms A and the B in the present application are not prone to staining, have low requirements for equipment, and are more suitable for industrial production; the stable properties are more conducive to quality control on industrial production and stability in drug efficacy.

Abstract: Provided herein are compositions, systems, methods, and kits related to chemical immunology for use in assessing and monitoring disease stages and phases, predicting the likelihood of disease progression, predicting and monitoring responses to disease therapies, and treating disease conditions.

Abstract: A system, for identifying post-translational modification in cross-linking mass spectrometry data, can comprise at least one processor, and at least one memory that stores executable instructions that, when executed by the at least one processor, facilitates performance of operations, comprising generating a peptide sequence tag graph based on a dataset of cross-linking spectral (XL-MS) data defining a real peptide set of one or more peptides and on a peptide database comprising information defining known peptides, based on a fuzzy string matching process applied to the peptide sequence tag graph, identifying a candidate peptide set corresponding to the real peptide set, identifying a post-translational modification (PTM) within the candidate peptide set, and scoring the PTM based on an aggregation of additional identifications of the PTM, wherein the scoring results in a PTM score assigned to the PTM that defines a probability of the PTM being comprised by the real peptide set.

Abstract: Provided is method for producing a phage library displaying antibodies or antibody fragments, comprising providing a first polynucleotide containing LC, a second polynucleotide containing connexon, and a third polynucleotide containing HC, respectively introducing the first, second and third polynucleotides to first, second and third bacteria to obtain a light chain component bacterial library, a connexon component bacterial library, and a heavy chain component bacterial library, obtaining a light chain component plasmid, a connexon component plasmid, and a heavy chain component plasmid from the libraries, obtaining the released LC, released connexon and released HC from the plasmids, connecting the released display vector segments to form a connection product for display, introducing a third bacterium to obtain a display bacterial library, and using the display bacterial library to prepare the phage library for displaying the antibodies or antibody fragments.

Abstract: The disclosure relates to a method of producing a multi-metal catalyst film and of producing a reactor system for catalytic removal of a wide variety of contaminants (for example, nitrate, nitrite, perchlorate, chlorate, chromate, selenate, chlorophenols, 2,4-D, dicamba, atrazine, trichloroacetic acid, bromochloroiodomethane, NDMA, TCE, TCA, chloroform, freons, RDX, HMX, TNT, PFOA, and PFOS) from water and wastewater. The disclosure also relates to a method of using the multi-metal catalyst for the removal of such contaminants and a system comprising the multi-metal catalyst film for removing such contaminants.

Abstract: The present invention is a rainfall prediction method, system, device and medium based on machine learning, which relates to the field of meteorological prediction technology. It uses atmospheric precipitable water volume (PWV) data, rainfall data and related meteorological parameters to input into a trained rainfall prediction network, and realizes accurate prediction of rainfall through an improved Transformer model. The model includes an encoder, a decoder and a final output layer, wherein feature extraction is performed inside the encoder through a multi-head probabilistic sparse self-attention module and a distillation module, and the encoder output containing feature information is used as the input of the decoder.

Abstract: Methods and systems are provided for ranking search results and generating a presentation. In some implementations, a search system generates a presentation based on a search query. In some implementations, a search system ranks search results based on data stored in a knowledge graph. In some implementations, a search system identifies a modifying concept such as a superlative in a received search query, and determines ranking properties based on the modifying concept.

Abstract: The present invention provides an antibody combined with human MASP-2, a preparation method therefor, and an application thereof. The monoclonal antibody of the present invention specifically binds to a MASP-2 antigen, and has high affinity, high specificity and high biological activity, thus having good clinical application prospects.

Abstract: Disclosed herein are systems and methods for removing trichloroethane (TCA), trichloroethene (TCE), and 1,4-dioxane (1,4-D) from contaminated liquids. The system and methods rely on catalyst reduction of TCA and TCE, where the reduced products are then degraded by microorganisms The system comprises a first reactor comprising a catalyst film of precious metal nanoparticles deposited on a first nonporous membrane and a second reactor comprising a biofilm of microorganisms that are capable of degrading ethane and 1,4-D deposited on a second nonporous membrane. The first reactor further comprises a hydrogen gas source, wherein the hydrogen gas source delivers hydrogen to the gas-phase side of the first nonporous membrane, and the catalyst film is deposited on the liquid-phase side. The second reactor further comprises an oxygen gas source, wherein the oxygen gas source delivers oxygen to the gas-phase side of the second non-porous membrane, and the biofilm is deposited on the liquid-phase side.

Abstract: This system presents a comprehensive approach for detecting protein-protein interactions and exploring protein structures. It encompasses various components, including a receptacle for receiving cross-linked precursor peptides, a mass spectrometer for generating MS1 and MS2 spectra, and modules for precursor mass refinement, MS2 spectrum scoring, protein score database construction, and feedback mechanism processing. Through the integration of these elements, the system facilitates the identification of peptide interactions and protein structures, leveraging a cross-linking dataset obtained from proteins cross-linked with cleavable or non-cleavable crosslinking reagents.

Abstract: A method for selecting a functional antigen-binding protein. The method comprises the step of constructing an expression vector, by means of a phage display technique and/or a cell display technique, using a light chain or a fragment thereof of a reference antigen-binding protein with a known sequence and function, or a heavy chain or a fragment thereof of the reference antigen-binding protein, thereby selecting a functional antigen-binding protein that binds to the same epitope as the reference antigen-binding protein. Further provided is a functional antigen-binding protein obtained by means of the method.

Abstract: The present invention relates to systems and methods for catalytic removal of per- and polyfluoroalkyl substances (PFAS) from water and wastewater. The system and methods utilize a catalyst film and a biofilm to synergystically remove PFAS from water. In some aspects, the catalyst film reduces and defluorinates PFAS into less fluorinated counterparts of PFAS, and the biofilm metabolizes the less fluroinated counterparts of PFAS into CO2 or shorter chain PFAS.