Abstract: The invention relates to a dry deposit as a precursor to liposome vesicles, the precursor being a three dimensional expanded structure with bulk density between 0.01 and 0.001 g/cm.sup.3. The invention also concerns a method of making liposome vesicles with an enhanced entrapment capacity by dissolving one or more film forming lipids in at least one organic solvent to form a solution in a reaction vessel, evaporating the solvent to form an expanded three dimensional porous lipid structure, contacting the lipid deposit with an aqueous carrier phase, and producing liposome vesicles entrapping the carrier phase as well as an apparatus comprising an array of tubing or an inert packing which serves as a material support or a matrix surface for the deposition of lipids produced according to the method.

Abstract: This device comprises, on the one hand, a stopper fixed to the neck of the container, having a skirt intended to do the fixing to the exterior wall of the container, a central hollow shaft resting against the internal face of the neck of the container and a closed end having an off-centered opening connected to the central hollow shaft by an oblique passage and, on the other hand, a cap which, mounted on the stopper, has an exterior skirt mounted so that it can pivot on the skirt of the stopper, a central hollow shaft engaged in the central hollow shaft of the stopper and in contact therewith, the skirt and the hollow shaft being connected by a closed end including an outlet opening for the product, which opening is situated the same distance away from the axis of rotation as the opening of the stopper, and the hollow shaft including an orifice formed on the same side as the opening in the closed end and at the same height as the oblique passage of the stopper.

Abstract: The invention relates to a dry deposit as a precursor to liposome vesicles, the precursor being a three dimensional expanded structure with bulk density between 0.01 and 0.001 g/cm.sup.3. The invention also concerns a method of making liposome vesicles with an enhanced entrapment capacity by dissolving one or more film forming lipids in at least one organic solvent to form a solution in a reaction vessel, evaporating the solvent to form an expanded three dimensional porous lipid structure, contacting the lipid deposit with an aqueous carrier phase, and producing liposome vesicles entrapping the carrier phase as well as an apparatus comprising an array of tubing or an inert packing which serves as a material support or a matrix surface for the deposition of lipids produced according to the method.

Abstract: The invention relates to a tamper-evident closure cap for a container having a neck and an opening defined in the neck. The closure cap of the invention includes an annular skirt member adapted to be secured to the neck of the container to prevent removal of the closure cap from the container, and a closure member hingedly connected to the skirt member for pivotal movement between a closed position whereat the closure member closes the opening of the container and an open position whereat the closure member permits access to the opening. A manually graspable tab is fixedly connected to the closure member for moving same from the closed position to the open position. The closure cap further includes a tamper-indicating member removably connected to the skirt member in overlying relation with respect to the tab and adapted to prohibit initial opening of the closure member prior to removal of the tamper indicating member.

Abstract: Liposome vesicles are prepared containing water or very dilute solutions encapsulated therein. These "empty" liposomes are suspended in a carrier liquid containing, dispersed therein, substances of interest to be loaded into the vesicles and incubated for a period of time at temperatures above the lipids transition temperature, whereby loading by transmembrane permeation occurs in high yields.

Abstract: Copolymer film deposited on a substrate comprising covalently fixed fragments of heparin having antithrombogenic properties. The polymer further comprises free carboxylic groups intended to neutralize free amine functions which are possibly present and to improve the hemocompatibility of the film.

Abstract: A ligand specific to a bioactive substance to be purified is fixed, via a connecting silane, to a mineral particulate carrier chosen from among SiO.sub.2, Al.sub.2 O.sub.3, ZrO.sub.2 and TiO.sub.2, the particles of the carrier being submicronic, non-porous and having a large specific surface. The carrier is contacted with an aqueous extract containing inter alia the bioactive substances, for the time required for the substance to become specifically fixed to the carrier. The carrier is then separated and the desired bioactive substance is isolated by desorption.

Abstract: A ligand specific to a bioactive substance to be purified is fixed, through a connecting silane, to a mineral particulate carrier chosen from among SiO.sub.2, Al.sub.2 O.sub.3, ZrO.sub.2 and TiO.sub.2, the particles of the carrier being submicronic, non-porous and having a large specific surface. The carrier is contacted with an aqueous extract containing the bioactive substances, for the time required for the substance to become specifically fixed to the carrier. The carrier is then separated and the desired bioactive substance is isolated by desorption.

Abstract: An acrylic-acid- based photopolymerizable composition is prepared which is capable of binding bioactive substances after being photopolymerized. The composition may be applied as a coating on a carrier substrate, photopolymerized and a bioactive substance fixed thereto. The composition adheres well to any usual carrier substrates, and its degree of hydrophilicity and permeability can be adapted to needs. The composition contains acrylic acid, a photoinitiator which is an aromatic ketone compound, a photopolymerization activator and adhesion promotor which is an amino-alcohol, acrylate or methacrylate, and a copolymerizable olefinic monomer which contains a reactive functional group capable of binding bioactive substances. The olefinic monomer is preferably N-hydroxysuccinimide acrylate, N-hydroxysuccinninimde amidocaproate, epoxypropyl acrylate or 2-isocyanato-ethyl acrylate.

Abstract: There is described a process for the production of a polysaccharide-producing enzyme, which comprises fermenting an appropriate enzyme producing organism in aqueous sucrose, characterized in that the sucrose concentration is maintained at between 1 and 10 g/l throughout all but the last part of the fermentation and that the dissolved oxygen content of the fermentation is controlled.There is also described a method for the purification of a polysaccharide producing enzyme, which comprises absorbing a mixture containing the enzyme on an anionic resin and removing the enzyme from the resin with a sugar containing eluant, and a novel highly purified form of polysaccharide producing enzyme.