Abstract: The present invention relates to methods of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.

Abstract: The skin plate product according to the invention is an adhesive, flat skin plate product for use as a semi-manufacture in the production of dressings, skin and wound care devices, fastening means for dressings, ostomy equipment, wound drains and catheters for incontinence equipment, in particular for men, and for use in electrodes for application to the skin, the said skin plate product having an area being delimited by the periphery of the product, and a first surface and a second surface consisting of two or more essentially non-mixed material units (1, 1', 2, 2', 2a, 2b, 2c, 4, 11, 12, 21, 21', 22, 22', 31, 32, 33, 41, 42, 43), at least two material units being of different material, of which at least one material unit is a skin friendly self-adhesive material, and where a first material unit (1, 1', 11, 21, 21', 31, 41) extends throughout the area of the entire product, this material unit further constituting at least a portion of the first surface (6, 26, 26', 36, 46) and the second surface (5, 25, 25',