Abstract: The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.

Abstract: The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.

Abstract: The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.

Abstract: The present invention relates to methods for editing the genome of a microbial host cell in one or more rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in a pooled and/or iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Compositions and kits for performing the methods are also disclosed.

Abstract: The present invention relates to methods for editing the genome of a microbial host cell in successive rounds of transformation. The method allows the introduction of genetic edits into the genome of a microbial host cell in an iterative fashion that does not require the use of functional counterselection following at least one round of transformation. It can be used to rapidly stack genetic edits in the genome of a microbial host cell. Kits for performing the methods are also disclosed.

Abstract: A TWIP steel sheet is provided having an austenitic matrix including by weight: 0.1?C?1.2%, 13.0?Mn<25.0%, 0.1?Si?3.0%, 0.1?Cu?5.0%, S?0.030%, P?0.080%, N?0.1%, 0.1?Al?4.0% and 0.1?V?2.50% in such way that:—when the amount of Al<2.0%, the weight ratio Al/V is between 0.2 and 8 or—when the amount of Al?2.0%, the amount of V>0.25%, and on a purely optional basis, one or more of Nb?0.5%, B?0.005%, Cr?1.0%, Mo?0.40%, Ni?1.0%, Ti?0.5%, and/or 0.06?Sn?0.2%, the remainder of the composition being made of iron and inevitable impurities resulting from elaboration.

Abstract: A cold rolled and recovered TWIP steel sheet is provided having an austenitic matrix including by weight: 0.71<C<1.2%, 13.0?Mn<25.0%, S?0.030%, P?0.080%, N?0.1%, 0.1?Si?3.0%, 0.1?V?2.50%, and on a purely optional basis, one or more elements such as Cu?5.0%, Al?4.0%, Nb?0.5%, B?0.005%, Cr?1.0%, Mo?0.40%, Ni?1.0%, Ti?0.5%, 0.06?Sn?0.2%, the remainder of the composition being made of iron and inevitable impurities resulting from elaboration.

Abstract: A hot-rolled or cold-rolled steel plate having a composition including, in weight percent: 0.6%?C?0.9%; 17%?Mn?22%; 0.2%?Al?0.9%; 0.2%?Si?1.1%; with 0.85%?Al+Si?1.6%; 1.2%?Cu?1.7%; S?0.030%; P?0.080%; N?0.1%; 0<Nb?0.25%; 0<V?0.5%; 0<Ti?0.5%; 0<Ni?2%; trace amounts?Cr?2% and B?0.010% is provided. A remainder of the composition includes iron and impurities resulting from production of the steel plate. A method for manufacturing a steel plate and use of the steel plate in the automotive industry is also provided.

Abstract: An austenitic steel sheet excellent in resistance to delayed cracking is provided. The composition of said steel comprises in weight: 0.35%?C?1.05% 15%?Mn?26% Si?3% Al?0.050% S?0.030% P?0.080% N?0.1%, at least one metallic element X chosen among vanadium, titanium, niobium, molybdenum, chromium 0.050%?V?0.50%, 0.040%?Ti?0.50% 0.070%?Nb?0.50% 0.14%?Mo?2% 0.070%?Cr?2%. The composition may optionally include B, Ni and/or Cu. The remainder of the composition includes iron and unavoidable impurities inherent to fabrication, including hydrogen. The quantity Xp of the at least one metallic element under the form of carbides, nitrides or carbonitrides is, in weight: 0.030%?Vp?0.40% 0.030%?Tip?0.50% 0.040%?Nbp?0.40% 0.14%?Mop?0.44% 0.070%?Crp?0.6%. The hydrogen content Hmax designating the maximal hydrogen content that can be measured from a series of at least five specimens, and the quantity Xp, in weight, is such that: 1000 ? H max X P ? 3.3 .

Abstract: The present disclosure relates to isolated enzyme complexes comprising a tethered cofactor and at least two enzymes paired to catalyse an enzymatic reaction and recycle the cofactor.

Abstract: A process for fabricating a steel sheet is provided. The process includes soaking a steel sheet. The steel has a composition including iron, carbon, manganese, silicon, aluminum, sulfur, phosphorus and nitrogen and at least one metallic element X chosen among vanadium, titanium, niobium, molybdenum, and chromium. A quantity Xp of metallic element under the form of carbides, nitrides or carbonitrides is, by weight: 0.030%?Vp?0.40%; 0.030%?Tip?0.50%; 0.040%?Nbp?0.40%; 0.14%?Mop?0.44%; or 0.070%?Crp?0.6%. The soaking step occurs under a pure nitrogen or argon atmosphere with a dew point lower than ?30° C. at a soaking temperature ? between 250 and 900° C. and with a dynamic circulation of a regenerated atmosphere.

Abstract: Systems and methods for data aggregation and analytics are disclosed. The systems query a participant for one or more behavioral and/or physical characteristics, receive data, and aggregate it into a participant profile. The disclosed systems and methods calculate the participant's performance based on said data and outputs the results of said calculation to one or more networked computers and/or one or more peripheral devices, associated with the participant or one or more individuals in charge of a study. The disclosed systems and methods are also capable of analyzing the participant data against historical data to identify correlations between the participant's data and adverse future health outcomes in real-time and clustering participants based on their behavioral and/or physical characteristics.

Abstract: A hot-rolled austenitic iron/carbon/manganese steel sheet is provided. The strength of which is greater than 900 MPa, the product (strength (in MPa)×elongation at fracture (in %)) of which is greater than 45000 and the chemical composition of which includes, the contents being expressed by weight 0.5%?C?0.7%, 17%?Mn?24%, Si?3%, Al?0.050%, S?0.030%, P?0.080% and N?0.1%. A remainder of the composition includes iron and inevitable impurities resulting from the smelting. A recrystallized fraction of the structure of the steel is greater than 75%, a surface fraction of precipitated carbides of the steel is less than 1.5% and a mean grain size of the steel is less than 18 microns. A reinforcing element is also provided.

Abstract: The present invention relates to enzymes capable of hydrolysing organophosphate (OP) molecules. In particular, the invention relates to variants of the OpdA enzyme from Agrobacterium that display improved activity when compared to the naturally occurring OpdA. The invention is also towards polypeptides that have organophosphate hydrolysing activity for the organophosphates chlorpyrifos methyl, diazinon and parathion ethyl.

Abstract: Steel sheet, the composition of the steel of which comprises, the contents being expressed by weight: 0.08%?C?0.23%, 1%?Mn?2%, 1?Si?2%, Al?0.030%, 0.1%?V?0.25%, Ti?0.010%, S?0.015%, P?0.1%, 0.004%?N?0.012%, and, optionally, one or more elements chosen from: Nb?0.1%, Mo?0.5%, Cr?0.3%, the balance of the composition consisting of iron and inevitable impurities resulting from the smelting.

Abstract: The present invention relates to pyruvate: ?-amino acid transaminases isolated from a Pseudomonas species. The transaminases act on long chain amino acids and are capable of accepting substrates comprising 8 to 12 carbon atoms. The enzymes are suitable as biocatalysts for the manufacture of nylon.

Abstract: A process for fabricating a steel sheet is provided. The process includes soaking a steel sheet. The steel has a composition including iron, carbon, manganese, silicon, aluminum, sulfur, phosphorus and nitrogen and at least one metallic element X chosen among vanadium, titanium, niobium, molybdenum, and chromium. A quantity Xp of metallic element under the form of carbides, nitrides or carbonitrides is, by weight: 0.030%?Vp?0.40%; 0.030%?Tip?0.50%; 0.040%?Nbp?0.40%; 0.14%?Mop?0.44%; or 0.070%?Crp?0.6%. The soaking step occurs under a pure nitrogen or argon atmosphere with a dew point lower than ?30° C. at a soaking temperature ? between 250 and 900° C. and with a dynamic circulation of a regenerated atmosphere.

Abstract: An austenitic steel sheet excellent in resistance to delayed cracking is provided. The composition of said steel comprises in weight: 0.35%?C?1.05% 15%?Mn?26% Si?3% Al?0.050% S?0.030% P?0.080% N?0.1%, at least one metallic element X chosen among vanadium, titanium, niobium, molybdenum, chromium 0.050%?V?0.50%, 0.040%?Ti?0.50% 0.070%?Nb?0.50% 0.14%?Mo?2% 0.070%?Cr?2%. The composition may optionally include B, Ni and/or Cu. The remainder of the composition includes iron and unavoidable impurities inherent to fabrication, including hydrogen. The quantity Xp of the at least one metallic element under the form of carbides, nitrides or carbonitrides is, in weight: 0.030%?Vp?0.40% 0.030%?Tip?0.50% 0.040%?Nbp?0.40% 0.14%?Mop?0.44% 0.070%?Crp?0.6%. The hydrogen content Hmax designating the maximal hydrogen content that can be measured from a series of at least five specimens, and the quantity Xp, in weight, is such that: 1000 ? H max X P ? 3.3 .

Abstract: An austenitic steel sheet excellent in resistance to delayed cracking, the composition of said steel comprising, in weight: 0,35%<C<1,05%, 15%<Mn<26%, Si<3%, Al<0,050%, S<0,030%, P<0,080%, N<0,1%, at least one metallic element X chosen among vanadium, titanium, niobium, molybdenum, chromium: 0,050%<V<0,50%, 0,040%?Ti<0,50%, 0,070%<Nb<0,50%, 0,14%<Mo<2%, 0,070%<Cr<2% and optionally, one or several elements chosen among 0,0005%<B<0,010%, Ni<2%, Cu<5%, the remainder being iron and unavoidable impurities inherent to fabrication, including hydrogen, the quantity Xp of said at least one metallic element under the form of carbides, nitrides or carbonitrides being, in weight: 0,030%<VP<0,40%, 0,030%<Tip<0,50%, 0,040%<Nbp<0,40%, 0,14%<Mop<0,44%, 0,070%<Crp<0,6%, the hydrogen content Hmax designating the maximal hydrogen content that can be measured from a series of at least five specimens, and the quantity Xp, in weight, be

Abstract: The present invention relates to pharmaceutical compositions comprising roflumilast in combination with a corticosteroid and/or a leukotriene receptor antagonist. The invention also relates to the use of such compositions for the treatment of Chronic Obstructive Pulmonary Disorder (COPD).