Abstract: A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.

Abstract: A secondary payload bridge for a payload adapter is disclosed and includes a body portion, plurality of payload ports, and a secondary payload port. The plurality of attachment points are connected to the body portion of the secondary payload bridge. The plurality of attachment points are configured to removably attach the secondary payload bridge to the payload adapter to allow for clockable positioning of the secondary payload bridge around a circumference of the payload adapter. The secondary payload port is connected to the body portion. The secondary payload port is configured to releasably attach to a corresponding secondary payload.

Abstract: A payload adapter configured to removably attach to one or more payload bridges. The payload adapter includes a forward open end defined by a forward ring, an aft open end defined by an aft ring, a plurality of truss supports connecting the forward ring and the aft ring to one another, and a plurality of mounting fixtures disposed around a circumference the payload adapter. The mounting fixtures are each configured to releasably attach to a secondary payload bridge.

Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.

Abstract: The present approach relates to the fabrication and use of a probe array having multiple individual probes. In one embodiment, the probes of the probe array may be functionalized such that certain of the probes are suitable for electrical sensing (e.g., recording) or stimulation, non-electrical sensing or stimulation (e.g., chemical sensing and/or release of biomolecules when activated), or a combination of electrical and non-electrical sensing or stimulation.

Abstract: A system for isolating a target molecule from a bioprocess fluid includes a single-use disposable separation device having a plurality of perimeter-bonded layers defining one or more mesofluidic channels of the separation device, wherein each layer includes a biocompatible polymer material, wherein the separation device is configured to separate at least a portion of particles from the bioprocess fluid to generate a substantially clarified bioprocess fluid, and a chromatography system fluidically coupled at the outflow of the separation device in a configuration for further processing the clarified bioprocess fluid.

Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.

Abstract: The subject matter of the present disclosure generally relates to a method and system for providing a bi-directional neural interface having an electrode configured to be positioned on a nerve; a substrate holding the electrode; a biocompatible insulator configured to electrically isolate the electrode when the electrode is positioned on the nerve, wherein the biocompatible insulator is formed in place about the electrode; and a pulse generator configured to deliver energy pulses to the electrode.

Abstract: The present approach relates to the fabrication of probes of a probe array device using wire bonding techniques. In certain implementation, a wire bond apparatus bonds ones end of a wire to a region of a probe array substrate. The second end, however, is not bonded to the substrate and instead is either fabricated to be vertical with respect to the substrate or raised from a non-bonded site to be vertical. The process may be repeated to form multiple probes of the probe array.

Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.

Abstract: A system includes a bacteria culture array that includes a plurality of chambers each configured to receive a portion of a sample that includes bacteria. Each individual chamber of the plurality of chambers includes a chamber opening configured to permit access of the portion of the sample to the individual chamber. The system also includes one or more sensors configured to collect data from the individual chamber. The sensors are configured to contact the sample. Additionally, the system includes a monitoring and analysis system that includes a processor configured to receive the data from the one or more sensors at a first time and a second time, compare the data received at the second time to the data received at the first time, and identify a portion of the plurality of chambers of the bacteria culture array based on the comparing.

Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.

Abstract: The present approach relates to the fabrication and use of a probe array having multiple individual probes. In one embodiment, the probes of the probe array may be functionalized such that certain of the probes are suitable for electrical sensing (e.g., recording) or stimulation, non-electrical sensing or stimulation (e.g., chemical sensing and/or release of biomolecules when activated), or a combination of electrical and non-electrical sensing or stimulation.

Abstract: A shark barrier includes a plurality of resiliently flexible, elongate members that extend in a generally upright condition between a sea floor and a sea surface. The elongate members are arranged so as to have the appearance of a thicket, preferably a kelp forest, when viewed from within the water and include magnets to assist in deterring certain shark species. The elongate members may be secured to the sea floor by an anchoring base and are held in the generally upright condition by a buoy or buoyant material held within the elongate members.

Abstract: A method of processing a transaction associated with a transaction instrument includes providing a server, receiving an update message at the server which includes data to assign a transaction use case associated with the transaction instrument, the server identifying a device user, a device or both and the associated transaction instrument, the server updating the stored transaction use case state associated with the transaction instrument, receiving a transaction request against the transaction instrument from a transaction requestor, the server classifying the transaction use case to determine a classified transaction use case associated with respect to the transaction instrument, the server determining whether the stored state of the classified transaction use case is an allowed or disallowed state, and transmitting a message to the transaction management system containing data confirming the state of the classified transaction use case, providing a general response of allowed or disallowed, or both.

Abstract: A separation device, system and associated method are provided herein for separation of particulates form a base fluid. The separation device comprises a first microchannel comprising a fluid inlet and a mesofluidic collection chamber. The mesofluidic collection chamber has a first side and a second side, wherein the mesofluidic collection chamber is operatively coupled to the first microchannel on the first side, and wherein the mesofluidic collection chamber comprises a first fluid outlet at the second side, such that the fluid inlet, first microchannel, and first fluid outlet are in fluidic communication via the mesofluidic collection chamber.

Abstract: The present disclosure relates to the manufacture and use of redox electrodes and their use in cell lysis. In certain embodiments, the redox electrodes are manufactured using a hybrid material approach, such as using a redox polymer in combination with a support substrate, such as cellulose fibers or paper. In certain implementations, the redox electrodes are suitable for use at voltages greater than 25 Volts.

Abstract: A method and system for data recovery in a parallel database system in which data stored in the database has been corrupted by a transaction or operation. Transaction log files corresponding to each node of the parallel database are scanned to determine an operation that caused the corruption of the stored data. Information corresponding to the corrupted data before the operation causing the corruption is then generated based on the scanned transaction log. The operation responsible for corrupting the data is then rolled back, and the corrupted data is replaced with the original data stored in the database prior to the corruption.

Abstract: The present disclosure relates to the manufacture and use of redox electrodes. In certain embodiments, the redox electrodes are manufactured using a hybrid material approach, such as using a redox polymer in combination with a support substrate, such as cellulose fibers or paper. In certain implementations, the redox electrodes are suitable for use at voltages greater than 25 V.

Abstract: A device and a system for eluting biomolecules from biological sample by electroelution are provided. The device for electroelution of biomolecules from a biological sample is constituted with a housing configured to receive an electrolyte and the biological sample, at least two electrodes comprising conductive redox polymers operationally coupled to the housing, and a biomolecule impermeable layer disposed on at least one of the electrodes. The biomolecule impermeable layer disposed on at least one of the electrodes to prevent the biomolecules from reaching the electrode. A system is provided, wherein the system comprises a sample collection port, one or more reservoirs comprising a buffer, a solvent, a reagent or combinations thereof, an device for electroelution, and a controller.