Abstract: The invention relates to a nucleic acid for use in DNA assembly, wherein the nucleic acid comprises at least one methylation-protectable restriction element, the methylation-protectable restriction element comprising: a restriction enzyme recognition sequence that is recognised by a restriction enzyme that cleaves outside of the recognition sequence; and a DNA methylase recognition sequence, wherein the restriction enzyme recognition sequence and the DNA methylase recognition sequence overlap such that the base modified by the DNA methylase lies within the restriction enzyme recognition sequence, wherein the DNA methylase recognition sequence is not identical to or enclosed by the restriction enzyme recognition sequence, and wherein the DNA methylase recognition sequence does not overlap with the sequence that would form the overhang end sequence generated by the restriction enzyme. The invention further relates to asscociated methods and kits.

Abstract: A heliostat frame includes a primary beam and several secondary beams arranged on the primary beam at intervals. The secondary beams are fixed on the primary beam along an extending direction of a center axis of the primary beam, and the secondary beam is provided with several supporting block assemblies. The supporting block assembly includes supporting blocks and adhesive sheets. The supporting blocks are connected with a reflective surface of the heliostat through the adhesive sheets. A height of each of the supporting blocks is configured according to its position on the secondary beam, so that a line connected by centers of top surfaces of all of the supporting blocks on the secondary beam is arc-shaped. The heliostat frame reduces the requirements for the manufacturing accuracy of the secondary beam while guaranteeing surface accuracy of the heliostat, thereby effectively reducing the production costs and improving the manufacturing efficiency.

Abstract: The invention relates to a nucleic acid comprising at least one methylation-protectable restriction element, the methylation-protectable restriction element comprising: (i) a type IIS restriction enzyme recognition sequence, or a partial type IIS restriction enzyme recognition sequence, that is recognised by a type IIS restriction enzyme that cleaves outside of the recognition sequence; (ii) a DNA methylase recognition sequence that is recognised and methylated by a DNA methylase, wherein the DNA methylase recognition sequence is identical to, or is encompassed within, the type IIS restriction recognition sequence, such that methylation of the nucleic acid by the DNA methylase methylates the type IIS restriction enzyme recognition sequence and protects the nucleic acid from cleavage by the type IIS restriction enzyme; and (iii) a recognition sequence for a sequence-specific DNA-binding protein, wherein the recognition sequence is positioned such that the binding of the sequence-specific DNA-binding protein ov

Abstract: A process for encapsulating a bowling pin includes placing a pin in a mold; injecting a molten plastic material into the mold to encapsulate the pin; and cooling the mold to produce a finished bowling pin wherein. The plastic material includes 80% to 60% of nylon and 20% to 40% of ion resin. Preferably, the ion resin is zinc ion resin.

Abstract: Video quality management (VQM) may be provided. First, transmitter metrics corresponding to a video stream may be determined by a transmitting endpoint. The determined transmitter metrics may then be transmitted from the transmitting end point and received by a receiver. The receiver may then determine receiver metrics for a video stream received at the receiver. A quality score may then be determined by the receiver. The quality score may be based on the received transmitter metrics and the determined receiver metrics.

Abstract: Video quality management (VQM) may be provided. First, transmitter metrics corresponding to a video stream may be determined by a transmitting endpoint. The determined transmitter metrics may then be transmitted from the transmitting end point and received by a receiver. The receiver may then determine receiver metrics for a video stream received at the receiver. A quality score may then be determined by the receiver. The quality score may be based on the received transmitter metrics and the determined receiver metrics.

Abstract: In a method of forming a micro pattern on a substrate, the micro pattern is predetermined to be formed in a first area of the substrate and not to be formed in a second area of the substrate spaced apart from the first area. A first shielding layer is formed on a surface of the substrate, and is configured to cover the second area and to expose the first area. A second shielding layer is formed on the surface of the substrate, is superimposed on the first shielding layer, and is configured to expose the first area of the substrate, and a portion of the first shielding layer. The first area of the substrate exposed from the second shielding layer is etched to form the micro pattern. The second shielding layer is then removed from the surface of the substrate and the first shielding layer. Finally, the first shielding layer is removed from the surface of the substrate.