Abstract: A PAM-less CRISPR/Cas gene editing system is provided. Specifically, the PAM-less CRISPR/Cas gene editing system offers Gs12-10, a PAM-less endonuclease identified through functional metagenomic mining approach, which can cleave target DNA at nearly any position in the genome. This technology enables a nucleic acid visualization detection and genome editing platform mediated by the CRISPR/Gs12-10 system, holding broad application potential in genome-modification and nucleic acid detection fields.

Abstract: An alginate fiber and a preparation method thereof are provided. The preparation method of the alginate fiber includes: S10: preparing a spinning solution with a raw material including sodium alginate; S20: extruding the spinning solution obtained in S10 into a solidification bath to allow solidification molding to obtain a primary fiber; S30: drawing and water-washing the primary fiber obtained in S20 to obtain an alginate fiber; and S40: soaking the alginate fiber obtained in S30 in a finishing agent to allow a post-treatment, where at least one of the raw material in S10, the solidification bath in S20, and the finishing agent in S30 includes a five-membered cyclic quaternary ammonium salt polymer. The alginate fiber obtained above has a high dye uptake, a small fiber strength loss, and a high soaping fastness.

Abstract: A nucleic acid endonuclease with high activity and high heat resistance and a gene editing system mediated by the nucleic acid endonuclease are provided. Specifically, the present invention provides a nucleic acid endonuclease Gs12-7 with a wide temperature range identified by metagenomics combined with experiments, which has the advantages of high protein temperature tolerance, recognition of PAM sequences containing BTYV, and thus has a larger gene editing space and high activity and specificity in cleaving target DNA in the genome. The present invention establishes a nucleic acid visualization detection and genome targeted editing technology mediated by the CRISPR/Gs12-7 system, which has broad application prospects in the field of genome targeted modification and nucleic acid detection.

Abstract: A method of calibrating capacitive array of a resistor-capacitor hybrid successive approximation register analog-to-digital converter (RC-hybrid SAR ADC) that includes a high M-bit capacitor DAC and a low N-bit resistor DAC. The method includes: disposing n unit capacitors in each capacitive array of the RC-hybrid SAR ADC, wherein n=2M?1; sorting the capacitors in an ascending order according to their capacitances to form a sorted array, and selecting two capacitors Cu(n/2)*, Cu(n/2+1)* in the middle positions as a least significant bit (LSB) capacitor and a dummy capacitor, respectively; obtaining a new array by forming each capacitor through adding two capacitors which have symmetrical positions with respect to the middle position(s) in the sorted array; and sorting the new array in an ascending order, and selecting the capacitor in the middle position as a higher bit capacitor. The method improves the static and dynamic performance of the SAR ADC.

Abstract: A method of calibrating capacitive array of a resistor-capacitor hybrid successive approximation register analog-to-digital converter (RC-hybrid SAR ADC) that includes a high M-bit capacitor DAC and a low N-bit resistor DAC. The method includes: disposing n unit capacitors in each capacitive array of the RC-hybrid SAR ADC, wherein n=2M?1; sorting the capacitors in an ascending order according to their capacitances to form a sorted array, and selecting two capacitors Cu(n/2)*, Cu(n/2+1)* in the middle positions as a least significant bit (LSB) capacitor and a dummy capacitor, respectively; 4) obtaining a new array by forming each capacitor through adding two capacitors which have symmetrical positions with respect to the middle position(s) in the sorted array; and sorting the new array in an ascending order, and selecting the capacitor in the middle position as a higher bit capacitor. The method improves the static and dynamic performance of the SAR.

Abstract: A method for improving a spurious free dynamic range and a signal-to-noise-and-distortion ratio of a capacitor-resistor combined successive approximation register analog-to-digital converter by capacitor re-configuration, the method including: 1) arranging 128 unit capacitors in a positive array and a negative array, respectively, dividing unit capacitors of symmetrical positions of the positive array and the negative array into groups to yield a total of 128 groups of capacitors; 2) acquiring 128 digital codes corresponding to 128 groups of capacitors; 3) sorting the 128 groups of capacitors from maximum to minimum according to the 128 digital codes obtained in 2), and recording the 128 groups of capacitors after sorting as C1-C128; and 4) selecting 64 groups of capacitors from C33 to C96, and reconfiguring the 64 groups of capacitors in capacitor arrays of the capacitor-resistor analog-to-digital converter.

Abstract: A method of arranging a capacitor array of a successive approximation register analog-to-digital converter in a successive approximation process, the method including: splitting a binary capacitor array into unit capacitors, then sorting, grouping, and rotating the original binary capacitive array involved in successive approximation conversion.

Abstract: A method of arranging a capacitor array of a successive approximation register analog-to-digital converter in a successive approximation process, the method including: splitting a binary capacitor array into unit capacitors, then sorting, grouping, and rotating the original binary capacitive array involved in successive approximation conversion.

Abstract: A method for improving a spurious free dynamic range and a signal-to-noise-and-distortion ratio of a capacitor-resistor combined successive approximation register analog-to-digital converter by capacitor re-configuration, the method including: 1) arranging 128 unit capacitors in a positive array and a negative array, respectively, dividing unit capacitors of symmetrical positions of the positive array and the negative array into groups to yield a total of 128 groups of capacitors; 2) acquiring 128 digital codes corresponding to 128 groups of capacitors; 3) sorting the 128 groups of capacitors from maximum to minimum according to the 128 digital codes obtained in 2), and recording the 128 groups of capacitors after sorting as C1-C128; and 4) selecting 64 groups of capacitors from C33 to C96, and reconfiguring the 64 groups of capacitors in capacitor arrays of the capacitor-resistor analog-to-digital converter.