Abstract: Provided are various systems and processes for improving last-mile delivery of real-time, on-demand orders for perishable goods. In one aspect, an automated vehicle (AV) comprises a body including a storage compartment for storing perishable goods. The storage compartment is accessible by a user upon authentication of the user. The AV further comprises a sensor module for receiving data for navigating the AV. The sensor module is positioned above the body on a support structure at a predetermined height above the ground, such as three to five feet. The data includes one or more of the following: audio data, video data, radio waves, and backscattered light waves. The AV further comprises an onboard computer system configured to process the data to navigate the AV along motor vehicle routes and pedestrian routes. The AV may be configured to interface with an automated locker system to retrieve or deposit the perishable goods.

Abstract: Provided are various systems and processes for improving last-mile delivery of real-time, on-demand orders for perishable goods. In one aspect, an automated vehicle (AV) comprises a body including a storage compartment for storing perishable goods. The storage compartment is accessible by a user upon authentication of the user. The AV further comprises a sensor module for receiving data for navigating the AV. The sensor module is positioned above the body on a support structure at a predetermined height above the ground, such as three to five feet. The data includes one or more of the following: audio data, video data, radio waves, and backscattered light waves. The AV further comprises an onboard computer system configured to process the data to navigate the AV along motor vehicle routes and pedestrian routes. The AV may be configured to interface with an automated locker system to retrieve or deposit the perishable goods.

Abstract: The present disclosure relates to powerful trans immune-modulatory systems, compositions and methods based on targeted genetic modification of immune-regulatory genes, for modulating immune cells and treating immune-related disorders. The disclosed systems comprise at least one donor nucleic acid molecule that encodes a replacement sequence that specifically integrates in a target sequence within a first immunoregulatory gene. The disclosed system further comprises at least one target recognition element, that targets the donor molecule to the target first immune-modulatory gene. The integration in the first target gene results in the production of at least one immune-modulatory product and/or modulation of the expression and/or activity of the immune-regulatory gene and any product thereof.

Abstract: The present disclosure provides nucleic acid guided genome modifier chimeric or fusion proteins, complexes or conjugate thereof, having enhanced homology-directed repair (HDR). The nucleic acid guided genome modifier disclosed herein comprise the following two components (a) at least one defective CRISPR-Cas protein (CRISPR-dCas) devoid of a nucleolytic activity or any variant or mutant thereof; and (b) at least one nucleic acid modifier component; and at least one of: (c) at least one donor attachment domain (DAD) for binding at least one Donor nucleic acid molecule; and (d) at least one repair factor recruitment domain (RFRD). The present disclosure further provides nucleic acid sequences encoding the nucleic acid guided genome modifier disclosed herein, as well as systems thereof and therapeutic and non-therapeutic methods thereof.

Abstract: Techniques regarding manufacturing one or more digital product designs are provided. For example, one or more embodiments described herein can include a system, which can comprise a memory that can store computer executable components. The system can also comprise a processor, operably coupled to the memory, and that can execute the computer executable components stored in the memory. The computer executable components can comprise a user interface component that generates a manufacturability report regarding a product design in relation to a manufacturing process. The manufacturability report can indicate whether a product feature included in the product design is permissible based on a plurality of manufacturing considerations associated with the manufacturing process.

Abstract: Techniques regarding manufacturing one or more digital product designs are provided. For example, one or more embodiments described herein can include a system, which can comprise a memory that can store computer executable components. The system can also comprise a processor, operably coupled to the memory, and that can execute the computer executable components stored in the memory. The computer executable components can comprise a user interface component that generates a manufacturability report regarding a product design in relation to a manufacturing process. The manufacturability report can indicate whether a product feature included in the product design is permissible based on a plurality of manufacturing considerations associated with the manufacturing process.

Abstract: Techniques regarding manufacturing one or more digital product designs are provided. For example, one or more embodiments described herein can include a system, which can comprise a memory that can store computer executable components. The system can also comprise a processor, operably coupled to the memory, and that can execute the computer executable components stored in the memory. The computer executable components can comprise a user interface component that generates a manufacturability report regarding a product design in relation to a manufacturing process. The manufacturability report can indicate whether a product feature included in the product design is permissible based on a plurality of manufacturing considerations associated with the manufacturing process.

Abstract: Apparatus is described including a tool, the tool including a shaft having a distal portion that is sized and shaped to be inserted into a subject during a surgical procedure. The tool includes a tissue-treatment tip, at the distal portion, that defines a beam deflector. An optical fiber is positioned to emit laser energy into the beam deflector. The beam deflector has a deflector surface, a first portion of which is shaped and positioned to reflect the laser energy toward a tissue-treatment tip surface, and a second portion of which is configured to absorb the laser energy and thermally conduct the absorbed energy to the tissue-treatment tip surface. The tissue-treatment tip surface is configured to thermally conduct the absorbed energy to the tissue by contacting the tissue. Other embodiments are also described.

Abstract: Provided herein are compositions and methods for modifying a predetermined nucleic acid sequence. A programmable nucleoprotein molecular complex containing a polypeptide moiety and a specificity conferring nucleic acid (SCNA) which assembles in-vivo, in a target cell, and is capable of interacting with the predetermined target nucleic acid sequence is provided. The programmable nucleoprotein molecular complex is capable of specifically modifying and/or editing a target site within the target nucleic acid sequence and/or modifying the function of the target nucleic acid sequence.

Abstract: Provided are various systems and processes for improving last-mile delivery of real-time, on-demand orders for perishable goods. In one aspect, an automated vehicle (AV) comprises a body including a storage compartment for storing perishable goods. The storage compartment is accessible by a user upon authentication of the user. The AV further comprises a sensor module for receiving data for navigating the AV. The sensor module is positioned above the body on a support structure at a predetermined height above the ground, such as three to five feet. The data includes one or more of the following: audio data, video data, radio waves, and backscattered light waves. The AV further comprises an onboard computer system configured to process the data to navigate the AV along motor vehicle routes and pedestrian routes. The AV may be configured to interface with an automated locker system to retrieve or deposit the perishable goods.

Abstract: Provided are various systems and processes for improving last-mile delivery of real-time, on-demand orders for perishable goods. In one aspect, a method is provided for aggregating on-demand deliveries using a depot dispatch protocol which may implement automated order transport and retrieval systems. The method comprises dispatching merchant couriers to transport on-demand orders from merchants to a merchant depot where the orders are aggregated and batched based on optimized delivery routes and destination proximities. Batches of orders are then transported to a customer depot corresponding to an area of delivery destinations. Orders are then assigned to delivery couriers for completion of delivery to customers. Such delivery routing systems and processes may be implemented alongside a delivery tracking system for generating estimated time of arrival predictive updates for real-time delivery of perishable goods.

Abstract: Provided are various systems and processes for improving last-mile delivery of real-time, on-demand orders for perishable goods. In one aspect, an automated vehicle (AV) comprises a body including a storage compartment for storing perishable goods. The storage compartment is accessible by a user upon authentication of the user. The AV further comprises a sensor module for receiving data for navigating the AV. The sensor module is positioned above the body on a support structure at a predetermined height above the ground, such as three to five feet. The data includes one or more of the following: audio data, video data, radio waves, and backscattered light waves. The AV further comprises an onboard computer system configured to process the data to navigate the AV along motor vehicle routes and pedestrian routes. The AV may be configured to interface with an automated locker system to retrieve or deposit the perishable goods.

Abstract: The invention provides highly effective and versatile CRISPR/Cas protein variants, compositions, methods and uses thereof in gene editing. More specifically, the invention relates to PAM-reduced or PAM-abolished Cas proteins and chimeras, complexes and conjugates thereof, genetic editing systems and to therapeutic and non-therapeutic methods and uses of the PAM-reduced or PAM-abolished Cas proteins.

Abstract: Provided herein are compositions and methods for modifying a predetermined nucleic acid sequence. A programmable nucleoprotein molecular complex containing a polypeptide moiety and a specificity conferring nucleic acid (SCNA) which assembles in-vivo, in a target cell, and is capable of interacting with the predetermined target nucleic acid sequence is provided. The programmable nucleoprotein molecular complex is capable of specifically modifying and/or editing a target site within the target nucleic acid sequence and/or modifying the function of the target nucleic acid sequence.

Abstract: A tool has a handle and an elongate shaft that extends distally from the handle. A distal portion of the shaft is inserted into a subject during a surgical procedure. An optical fiber delivers laser energy to a tip at the distal portion of the shaft. The tip includes a mechanical cutting mechanism including a moving part that absorbs the laser energy, thermally conducts the absorbed energy to tissue that is disposed between the moving part and another part, and moves with respect to the other part in order to cut tissue that is disposed between the parts using a mechanical force that is lower than a mechanical force that would be required to cut the tissue in the absence of the laser energy. Other embodiments are also described.

Abstract: Provided are various mechanisms and processes for optimizing real-time, on-demand delivery service for perishable goods. In one aspect, a method is provided for aggregating on-demand deliveries using a depot dispatch protocol. The method comprises dispatching runners to pick up on-demand orders for drop off at a depot where the orders are aggregated and batched based on optimized delivery routes and destination proximities. Batches of orders are then assigned to couriers who may arrive at the depot to receive the batched orders without having to navigate through areas of high congestion. Such delivery routing system may be implemented alongside a delivery tracking system for generating estimated time of arrival predictive updates for real-time delivery of perishable goods.

Abstract: Provided herein are compositions and methods for modifying a predetermined nucleic acid sequence. A programmable nucleoprotein molecular complex containing a polypeptide moiety and a specificity conferring nucleic acid (SCNA) which assembles in-vivo, in a target cell, and is capable of interacting with the predetermined target nucleic acid sequence is provided. The programmable nucleoprotein molecular complex is capable of specifically modifying and/or editing a target site within the target nucleic acid sequence and/or modifying the function of the target nucleic acid sequence.

Abstract: Provided are various systems and processes for improving last-mile delivery of real-time, on-demand orders for perishable goods. In one aspect, an automated vehicle (AV) comprises a body including a storage compartment for storing perishable goods. The storage compartment is accessible by a user upon authentication of the user. The AV further comprises a sensor module for receiving data for navigating the AV. The sensor module is positioned above the body on a support structure at a predetermined height above the ground, such as three to five feet. The data includes one or more of the following: audio data, video data, radio waves, and backscattered light waves. The AV further comprises an onboard computer system configured to process the data to navigate the AV along motor vehicle routes and pedestrian routes. The AV may be configured to interface with an automated locker system to retrieve or deposit the perishable goods.

Abstract: A tool has a handle and an elongate shaft that extends distally from the handle. A distal portion of the shaft is inserted into a subject during a surgical procedure. An optical fiber delivers laser energy to a tip at the distal portion of the shaft. The tip includes a mechanical cutting mechanism including a moving part that absorbs the laser energy, thermally conducts the absorbed energy to tissue that is disposed between the moving part and another part, and moves with respect to the other part in order to cut tissue that is disposed between the parts using a mechanical force that is lower than a mechanical force that would be required to cut the tissue in the absence of the laser energy. Other embodiments are also described.

Abstract: Techniques regarding manufacturing one or more digital product designs are provided. For example, one or more embodiments described herein can include a system, which can comprise a memory that can store computer executable components. The system can also comprise a processor, operably coupled to the memory, and that can execute the computer executable components stored in the memory. The computer executable components can comprise a user interface component that generates a manufacturability report regarding a product design in relation to a manufacturing process. The manufacturability report can indicate whether a product feature included in the product design is permissible based on a plurality of manufacturing considerations associated with the manufacturing process.