Patents by Inventor Daniel T. Chiu

Daniel T. Chiu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220356512
    Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.
    Type: Application
    Filed: July 19, 2022
    Publication date: November 10, 2022
    Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Daniel T. CHIU, Jason E. KREUTZ, Gloria S. YEN, Bryant S. FUJIMOTO
  • Patent number: 11480575
    Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.
    Type: Grant
    Filed: December 3, 2019
    Date of Patent: October 25, 2022
    Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Daniel T. Chiu, Mengxia Zhao, Wyatt Nelson, Perry G. Schiro
  • Patent number: 11427857
    Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: August 30, 2022
    Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Daniel T. Chiu, Jason E. Kreutz, Gloria S. Yen, Bryant S. Fujimoto
  • Publication number: 20220252607
    Abstract: The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.
    Type: Application
    Filed: February 15, 2022
    Publication date: August 11, 2022
    Inventors: Daniel T. Chiu, Changfeng Wu, Jason McNeill, Jiangbo Yu
  • Patent number: 11408884
    Abstract: Lyophilized chromophoric polymer dot compositions are provided. Also disclosed are methods of making and using the lyophilized compositions, methods of dispersing the lyophilized compositions in aqueous solutions and kits supplying the compositions.
    Type: Grant
    Filed: December 3, 2019
    Date of Patent: August 9, 2022
    Assignee: University of Washington through its Center for Commercialization
    Inventors: Daniel T. Chiu, Wei Sun, Jiangbo Yu, Changfeng Wu, Fangmao Ye
  • Patent number: 11408903
    Abstract: Systems, methods, and devices for discretizing and analyzing fluidic samples are provided. In one aspect, a microfluidic array for discretizing a fluidic sample comprises one or more flow channels and a plurality of fluidic compartments in fluidic communication with the one or more flow channels. In another aspect, a system for discretizing and analyzing fluidic samples comprises a rotor assembly shaped to receive a microfluidic device.
    Type: Grant
    Filed: August 31, 2020
    Date of Patent: August 9, 2022
    Assignee: University of Washington
    Inventors: Daniel T. Chiu, Bryant S. Fujimoto, Jason E. Kreutz
  • Patent number: 11401547
    Abstract: Methods and systems for digital measurements are provided. In an embodiment, the method includes producing a plurality of droplets, wherein at least one of the droplets of the plurality of droplets contains an analyte molecule from a sample; measuring at least a first portion of the plurality of droplets to determine individual volumes of droplets in the first portion of the plurality of droplets; analyzing at least a second portion of the plurality of droplets to determine a number of droplets in the second portion of the plurality of droplets that contain the analyte molecule; and using individual volumes of the droplets in the first portion of the plurality of droplets and the number of droplets in the second portion of the plurality of droplets that contain the analyte molecule to determine the concentration of the analyte molecule in the sample.
    Type: Grant
    Filed: February 5, 2020
    Date of Patent: August 2, 2022
    Assignee: University of Washington through its Center for Commercialization
    Inventors: Daniel T. Chiu, Bryant S. Fujimoto, Alexander R. Gansen, Gloria S. Yen, Robert M. Lorenz
  • Publication number: 20220097043
    Abstract: Devices, systems and apparatuses for the discretization and manipulation of sample volumes are provided. Related methods are also provided.
    Type: Application
    Filed: November 23, 2021
    Publication date: March 31, 2022
    Applicant: University of Washington through its Center for Commercialization
    Inventors: Daniel T. Chiu, Thomas Schneider, Jason E. Kreutz
  • Publication number: 20220082566
    Abstract: The present disclosure provides encoded chromophoric polymer particles that are capable of, for example, optical and/or biomolecular encoding of analytes. The present disclosure also provides suspensions comprising a plurality of encoded chromophoric polymer particles. The present disclosure also provides methods of using the encoded chromophoric polymer particles and systems for performing multiplex analysis with encoded chromophoric polymer particles.
    Type: Application
    Filed: November 30, 2021
    Publication date: March 17, 2022
    Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Daniel T. Chiu, Changfeng Wu, Jiangbo Yu
  • Patent number: 11249086
    Abstract: The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.
    Type: Grant
    Filed: December 4, 2018
    Date of Patent: February 15, 2022
    Assignee: UNIVERSITY OF WASHINGTON
    Inventors: Daniel T. Chiu, Changfeng Wu, Jason McNeill, Jiangbo Yu
  • Patent number: 11249073
    Abstract: The present disclosure provides metal-containing (MC) semiconducting (SC) Pdots (MC-SC-Pdots) with beneficial functionalities in both cellular imaging and manipulation, among other applications. The Pdots comprise at least one nanoparticle comprising at least one metal, and a semiconducting polymer associated with the nanoparticle.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: February 15, 2022
    Assignee: UNIVERSITY OF WASHINGTON
    Inventors: Daniel T. Chiu, Wei Sun, Jiangbo Yu, Changfeng Wu
  • Publication number: 20220025111
    Abstract: Polymers, monomers, narrow-band absorbing polymers, narrow-band absorbing monomers, absorbing units, polymer dots, and related methods are provided. Bright, luminescent polymer nanoparticles with narrow-band absorptions are provided. Methods for synthesizing absorbing monomers, methods for synthesizing the polymers, preparation methods for forming the polymer nanoparticles, and applications for using the polymer nanoparticles are also provided.
    Type: Application
    Filed: September 16, 2019
    Publication date: January 27, 2022
    Applicant: University of Washington
    Inventors: Daniel T. Chiu, Lei Chen, Jiangbo Yu
  • Patent number: 11221336
    Abstract: The present disclosure provides encoded chromophoric polymer particles that are capable of, for example, optical and/or biomolecular encoding of analytes. The present disclosure also provides suspensions comprising a plurality of encoded chromophoric polymer particles. The present disclosure also provides methods of using the encoded chromophoric polymer particles and systems for performing multiplex analysis with encoded chromophoric polymer particles.
    Type: Grant
    Filed: August 29, 2019
    Date of Patent: January 11, 2022
    Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Daniel T. Chiu, Changfeng Wu, Jiangbo Yu
  • Patent number: 11219896
    Abstract: Devices, systems and apparatuses for the discretization and manipulation of sample volumes are provided. Related methods are also provided.
    Type: Grant
    Filed: June 25, 2014
    Date of Patent: January 11, 2022
    Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION
    Inventors: Daniel T. Chiu, Thomas Schneider, Jason E. Kreutz
  • Publication number: 20210403990
    Abstract: The present disclosure provides a method of measuring the quantity of analyte molecules. In some aspects, the method comprises compartmentalizing a sample with binding molecules conjugated to synthetic nucleic acid molecules such that the interaction of the binding molecules with the analyte molecules brings the nucleic acid molecules into proximity. Proximity triggers reactions that result in an optical signal, such as fluorescence, in analyte-containing compartments which can be counted to determine the quantity of analyte present.
    Type: Application
    Filed: September 25, 2019
    Publication date: December 30, 2021
    Inventors: Daniel T. CHIU, Peter ALLEN
  • Publication number: 20210343445
    Abstract: The present disclosure provides organic-inorganic hybrid polymer particles, which have desirable surface chemistry and optical properties that make them particularly suitable for biological and optical applications. The present disclosure also provides methods of making organic-inorganic hybrid polymer particles. The present disclosure also provides methods of using the organic-inorganic hybrid polymer particles for biological and optical applications.
    Type: Application
    Filed: July 6, 2021
    Publication date: November 4, 2021
    Applicant: University of Washington
    Inventors: Daniel T. CHIU, Jiangbo YU, Yu RONG, Changfeng WU
  • Patent number: 11087900
    Abstract: The present disclosure provides organic-inorganic hybrid polymer particles, which have desirable surface chemistry and optical properties that make them particularly suitable for biological and optical applications. The present disclosure also provides methods of making organic-inorganic hybrid polymer particles. The present disclosure also provides methods of using the organic-inorganic hybrid polymer particles for biological and optical applications.
    Type: Grant
    Filed: June 12, 2020
    Date of Patent: August 10, 2021
    Assignee: University of Washington
    Inventors: Daniel T. Chiu, Jiangbo Yu, Yu Rong, Changfeng Wu
  • Publication number: 20210172957
    Abstract: Methods, systems, compositions and kits are provided for the analysis of target molecules using chromophoric polymer dots conjugated to biomolecules. The use of chromophoric polymer dots improves detection sensitivity and stability when compared with existing techniques. In some aspects, methods, systems, and kits are provided for detecting a target protein using chromophoric polymer dots conjugated to biomolecules in a Western blot analysis. Related methods, systems, compositions and kits are also provided.
    Type: Application
    Filed: February 12, 2021
    Publication date: June 10, 2021
    Applicant: University of Washington
    Inventor: Daniel T. Chiu
  • Publication number: 20210087618
    Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the digital assays comprise compartmentalized volumes. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume.
    Type: Application
    Filed: January 22, 2019
    Publication date: March 25, 2021
    Inventors: Daniel T. CHIU, Jason E. KREUTZ
  • Patent number: 10955419
    Abstract: Methods, systems, compositions and kits are provided for the analysis of target molecules using chromophoric polymer dots conjugated to biomolecules. The use of chromophoric polymer dots improves detection sensitivity and stability when compared with existing techniques. In some aspects, methods, systems, and kits are provided for detecting a target protein using chromophoric polymer dots conjugated to biomolecules in a Western blot analysis. Related methods, systems, compositions and kits are also provided.
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: March 23, 2021
    Assignee: University of Washington
    Inventor: Daniel T. Chiu