Patents by Inventor Daniel T. Chiu
Daniel T. Chiu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11939626Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.Type: GrantFiled: July 19, 2022Date of Patent: March 26, 2024Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATIONInventors: Daniel T. Chiu, Jason E. Kreutz, Gloria S. Yen, Bryant S. Fujimoto
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Publication number: 20240069032Abstract: The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.Type: ApplicationFiled: October 23, 2023Publication date: February 29, 2024Inventors: Daniel T. Chiu, Changfeng Wu, Jason McNeil, Jiangbo Yu
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Publication number: 20240047098Abstract: The present disclosure provides organic-inorganic hybrid polymer particles, which have desirable surface chemistry and optical properties that make them particularly suitable for biological and optical applications. The present disclosure also provides methods of making organic-inorganic hybrid polymer particles. The present disclosure also provides methods of using the organic-inorganic hybrid polymer particles for biological and optical applications.Type: ApplicationFiled: October 11, 2023Publication date: February 8, 2024Applicant: University of WashingtonInventors: Daniel T. CHIU, Jiangbo YU, Yu RONG, Changfeng WU
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Publication number: 20240027325Abstract: Systems and methods for analyzing particles flowing in a channel are described. In an embodiment, the channel is configured to flow a particle through a lumen of the channel, the channel defining an interrogation window configured to allow light to pass into and out of the lumen; a light engine comprising: a first light source positioned to output first excitation light onto a first portion of the channel in the interrogation window; and a second light source positioned to output second excitation light onto a second portion of the channel in the interrogation window separate from the first portion. In an embodiment, the systems include an emission fiber bundle comprising a first emission optical fiber and a second emission optical fiber, wherein a proximal end of first emission optical fiber and second emission optical fiber are arranged in an emission fiber bundle head.Type: ApplicationFiled: November 10, 2021Publication date: January 25, 2024Applicant: University of WashingtonInventors: Daniel T. Chiu, Yifei Jiang, Mengxia Zhao, Yuanhua Cheng
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Publication number: 20230408526Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.Type: ApplicationFiled: November 23, 2022Publication date: December 21, 2023Applicant: University of Washington through its Center for CommercializationInventors: Daniel T. Chiu, Changfeng Wu, Xuanjun Zhang, Jiangbo Yu, Fangmao Ye
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Patent number: 11835526Abstract: The present invention provides, among other aspects, functionalized chromophoric polymer dots comprising a hydrophobic core and a hydrophilic cap, and bioconjugates thereof. Also provided are improved methods for preparing functionalized chromophoric polymer dots. Methods for in vivo imaging and molecular labeling are also disclosed.Type: GrantFiled: February 15, 2022Date of Patent: December 5, 2023Assignees: University of Washington, Clemson University Research FoundationInventors: Daniel T. Chiu, Changfeng Wu, Jason McNeill, Jiangbo Yu
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Patent number: 11823809Abstract: The present disclosure provides organic-inorganic hybrid polymer particles, which have desirable surface chemistry and optical properties that make them particularly suitable for biological and optical applications. The present disclosure also provides methods of making organic-inorganic hybrid polymer particles. The present disclosure also provides methods of using the organic-inorganic hybrid polymer particles for biological and optical applications.Type: GrantFiled: July 6, 2021Date of Patent: November 21, 2023Assignee: University of WashingtonInventors: Daniel T. Chiu, Jiangbo Yu, Yu Rong, Changfeng Wu
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Patent number: 11808767Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.Type: GrantFiled: September 16, 2022Date of Patent: November 7, 2023Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATIONInventors: Daniel T. Chiu, Mengxia Zhao, Wyatt Nelson, Perry G. Schiro
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Patent number: 11753681Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the detection of nucleic acids and proteins. Also provided are methods, devices, systems, and compositions for improved detection and quantification of target molecules using encoded probes. In certain aspects, the methods, devices, and systems provided herein are useful in multiplexed digital assays. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of compartmentalized volumes in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.Type: GrantFiled: November 1, 2018Date of Patent: September 12, 2023Assignee: UNIVERSITY OF WASHINGTONInventors: Daniel T. Chiu, Jiangbo Yu, Jason E. Kreutz, Jiasi Wang
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Publication number: 20230251258Abstract: Methods and systems for improved labeling and/or de-labeling a molecule or cell in the context of scientific experimentation, industrial applications, and clinical investigation, including the means to repeat the process of labeling and de-labeling in an efficient manner.Type: ApplicationFiled: April 20, 2023Publication date: August 10, 2023Applicant: University of WashingtonInventors: Daniel T. Chiu, Chun-Ting Kuo, Li Wu
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Patent number: 11697713Abstract: Polymers, monomers, chromophoric polymer dots and related methods are provided. Highly fluorescent chromophoric polymer dots with narrow-band emissions are provided. Methods for synthesizing the chromophoric polymers, preparation methods for forming the chromophoric polymer dots, and biological applications using the unique properties of narrow-band emissions are also provided.Type: GrantFiled: September 21, 2018Date of Patent: July 11, 2023Assignee: University of Washington through its Center for CommercializationInventors: Daniel T. Chiu, Changfeng Wu, Yu Rong, Yong Zhang, Yi-Che Wu, Yang-Hsiang Chan, Xuanjun Zhang, Jiangbo Yu, Wei Sun
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Patent number: 11674964Abstract: The present disclosure provides encoded chromophoric polymer particles that are capable of, for example, optical and/or biomolecular encoding of analytes. The present disclosure also provides suspensions comprising a plurality of encoded chromophoric polymer particles. The present disclosure also provides methods of using the encoded chromophoric polymer particles and systems for performing multiplex analysis with encoded chromophoric polymer particles.Type: GrantFiled: November 30, 2021Date of Patent: June 13, 2023Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATIONInventors: Daniel T. Chiu, Changfeng Wu, Jiangbo Yu
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Patent number: 11668713Abstract: Methods and systems for improved labeling and/or de-labeling a molecule or cell in the context of scientific experimentation, industrial applications, and clinical investigation, including the means to repeat the process of labeling and de-labeling in an efficient manner.Type: GrantFiled: November 16, 2017Date of Patent: June 6, 2023Assignee: UNIVERSITY OF WASHINGTONInventors: Daniel T. Chiu, Chun-Ting Kuo, Li Wu
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Publication number: 20230159979Abstract: Transducers, kits, systems, and methods for determining a concentration of an analyte are described. In an embodiment, the transducers include a chromophore; and an enzyme physically associated with the chromophore. In an embodiment, the transducer is configured to catalyze a reaction comprising a plurality of reaction elements. In an embodiment, the plurality of reaction elements comprises one or more reactants including the analyte and one or more products. In an embodiment, an amount of fluorescence emitted from the chromophore is determined by a concentration of a reaction element of the plurality of reaction elements.Type: ApplicationFiled: April 15, 2021Publication date: May 25, 2023Applicants: UNIVERSITY OF WASHINGTON, LAMPROGEN, INC.Inventors: Daniel T. Chiu, Haobin Chen, Jiangbo Yu
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Patent number: 11648560Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and can be contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.Type: GrantFiled: August 15, 2018Date of Patent: May 16, 2023Assignees: University of Washnington, Micareo Inc.Inventors: Daniel T. Chiu, Mengxia Zhao, Eleanor S. Shah, Perry G. Schiro, Hui Min Yu, Wei-Feng Fang, Jui-Lin Chen
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Publication number: 20230077385Abstract: The present disclosure provides nanoparticle transducers and methods of use thereof for the detection of analyte concentrations in a fluid. Nanoparticle transducers can comprise a nanoparticle, such as a Pdot, coupled to an enzyme that catalyzes a reaction with the analyte. The nanoparticle transducers further comprise chromophores that emit fluorescence that varies as a function of the concentration of one of the elements of the reaction. The nanoparticle transducer thus changes fluorescence as the analyte concentration changes, transforming analyte concentration values into fluorescence intensities. The measurement of these intensities provides a measurement of the analyte concentration. The nanoparticle transducers are biocompatible, allowing for use in vivo, for the monitoring of analyte blood concentrations such as blood glucose concentrations.Type: ApplicationFiled: November 23, 2022Publication date: March 16, 2023Applicants: University of Washington, LAMPROGEN, INC.Inventors: Daniel T. Chiu, Jiangbo Yu, Changfeng Wu, Kai Sun
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Patent number: 11585818Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.Type: GrantFiled: August 10, 2020Date of Patent: February 21, 2023Assignee: University of Washington through its Center for CommercializationInventors: Daniel T. Chiu, Changfeng Wu, Xuanjun Zhang, Jiangbo Yu, Fangmao Ye
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Publication number: 20230023536Abstract: Provided herein, among other aspects, are methods and apparatuses for analyzing particles in a sample. In some aspects, the particles can be analytes, cells, nucleic acids, or proteins and contacted with a tag, partitioned into aliquots, detected by a ranking device, and isolated. The methods and apparatuses provided herein may include a microfluidic chip. In some aspects, the methods and apparatuses may be used to quantify rare particles in a sample, such as cancer cells and other rare cells for disease diagnosis, prognosis, or treatment.Type: ApplicationFiled: September 16, 2022Publication date: January 26, 2023Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATIONInventors: Daniel T. CHIU, Mengxia ZHAO, Wyatt NELSON, Perry G. SCHIRO
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Patent number: 11542539Abstract: The present disclosure provides nanoparticle transducers and methods of use thereof for the detection of analyte concentrations in a fluid. Nanoparticle transducers can comprise a nanoparticle, such as a Pdot, coupled to an enzyme that catalyzes a reaction with the analyte. The nanoparticle transducers further comprise chromophores that emit fluorescence that varies as a function of the concentration of one of the elements of the reaction. The nanoparticle transducer thus changes fluorescence as the analyte concentration changes, transforming analyte concentration values into fluorescence intensities. The measurement of these intensities provides a measurement of the analyte concentration. The nanoparticle transducers are biocompatible, allowing for use in vivo, for the monitoring of analyte blood concentrations such as blood glucose concentrations.Type: GrantFiled: June 5, 2017Date of Patent: January 3, 2023Assignees: UNIVERSITY OF WASHINGTON, LAMPROGEN, INC.Inventors: Daniel T. Chiu, Jiangbo Yu, Changfeng Wu, Kai Sun
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Publication number: 20220356512Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.Type: ApplicationFiled: July 19, 2022Publication date: November 10, 2022Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATIONInventors: Daniel T. CHIU, Jason E. KREUTZ, Gloria S. YEN, Bryant S. FUJIMOTO