Abstract: This invention provides isolated nucleic acid encoding sperm-specific protein kinase A anchoring proteins from mammalian species, recombinant expression constructs encoding said mammalian sperm-specific protein kinase A anchoring proteins, cells transformed with said constructs, and homogenous preparations of these proteins prepared using recombinant genetic techniques. The invention also provides analytic tools such as polyclonal antisera and monoclonal antibodies specific for said sperm-specific protein kinase A anchoring proteins of the invention. The invention also provides methods for isolating nucleic acid encoding mammalian sperm-specific protein kinase A anchoring proteins and recombinant genetic methods for producing said mammalian sperm-specific protein kinase A anchoring proteins of the invention.

Abstract: This invention provides isolated nucleic acid encoding sperm-specific protein kinase A anchoring proteins from mammalian species, recombinant expression constructs encoding said mammalian sperm-specific protein kinase A anchoring proteins, cells transformed with said constructs, and homogenous preparations of these proteins prepared using recombinant genetic techniques. The invention also provides analytic tools such as polyclonal antisera and monoclonal antibodies specific for said sperm-specific protein kinase A anchoring proteins of the invention. The invention also provides methods for isolating nucleic acid encoding mammalian sperm-specific protein kinase A anchoring proteins and recombinant genetic methods for producing said mammalian sperm-specific protein kinase A anchoring proteins of the invention.

Abstract: The present invention includes a pharmaceutical composition having an effective contraceptive amount of a synthetic peptide that includes an amphipathic .alpha.-helix domain that binds to an RII subunit of protein kinase A, and competitively inhibits the binding of protein kinase A to sperm A kinase anchoring proteins. Particular disclosed synthetic peptides having this activity include s-Ht31: N-Stearate-DLIEEAASRIVDAVIEQVKAAGAY (SEQ ID No. 9), s-Ht31-P: N-Stearate-DLIEEAASRPVDAVPEQVKAAGAY (SEQ ID No. 10), and s-AKAP79: N-Stearate-YETLLIETASSLVKNAIQLSIE (SEQ ID No. 11). The invention also includes methods of inhibiting sperm motility, by exposing them to an effective amount of the peptide, for example by placing the pharmaceutical composition (such as a suppository, foam, cream, or gel) in the vagina.