Patents by Inventor David B. Krizman

David B. Krizman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Isolation of cellular material under microscopic visualization
    Patent number: 6251516
    Abstract: A method of microdissection which involves forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes a selectively activatable adhesive layer which provides, for example, chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated, the transfer surface and tissue sample are separated.
    Type: Grant
    Filed: February 4, 1998
    Date of Patent: June 26, 2001
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Robert F. Bonner, Lance A. Liotta, Michael Emmert-Buck, David B. Krizman, Rodrigo Chuaqui, W. Marston Linehan, Jeffry M. Trent, Seth R. Goldstein, Paul D. Smith, John I. Peterson
  • Isolation of cellular material under microscopic visualization
    Patent number: 6251467
    Abstract: A method of microdissection which involves forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes a selectively activatable adhesive layer which provides, for example, chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated, the transfer surface and tissue sample are separated.
    Type: Grant
    Filed: July 29, 1999
    Date of Patent: June 26, 2001
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Lance A. Liotta, Michael Emmert-Buck, David B. Krizman, Rodrigo Chuaqui, W. Marston Linehan, Jeffry M. Trent, Robert F. Bonner, Seth R. Goldstein, Paul D. Smith, John I. Peterson