Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleotide connected to a nucleic acid comprising a FRET label comprising at least three luminescent molecules. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the disclosure provide biconjugatable labels, labeled biomolecules, and methods of using and making the same.

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

Abstract: A building occupant tracker for tracking the location of an occupant in a building by tracking a smart device associated with the occupant at a location server.

Abstract: A connected traffic monitoring system comprises at least one Roadside Unit (RSU) and a traffic signal controller. The roadside unit is configured to transmit wireless signals, receive corresponding responses from a first Onboard Unit (OBU)-equipped vehicle and a second OBU-equipped vehicle and send data from the first OBU-equipped vehicle and the second OBU-equipped vehicle to the traffic signal controller. The traffic signal controller to calculate a distance between the first Onboard Unit (OBU)-equipped vehicle and the second OBU-equipped vehicle in a vehicle queue associated with a traffic light signal on an intersection, determine the queue length of the vehicle queue, determine whether the distance between the first OBU-equipped vehicle and the second OBU-equipped vehicle is greater than a vehicle length and if the distance is determined greater than the vehicle length, detect at least one non-OBU-equipped vehicle stopped in the vehicle queue behind the first OBU-equipped vehicle.

Abstract: Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction. In some aspects, the disclosure provides compositions comprising luminescently labeled nucleotides.

Abstract: A connected traffic monitoring system comprises at least one Roadside Unit (RSU) and a traffic signal controller. The roadside unit is configured to transmit wireless signals, receive corresponding responses from a first Onboard Unit (OBU)-equipped vehicle and a second OBU-equipped vehicle and send data from the first OBU-equipped vehicle and the second OBU-equipped vehicle to the traffic signal controller. The traffic signal controller to calculate a distance between the first Onboard Unit (OBU)-equipped vehicle and the second OBU-equipped vehicle in a vehicle queue associated with a traffic light signal on an intersection, determine the queue length of the vehicle queue, determine whether the distance between the first OBU-equipped vehicle and the second OBU-equipped vehicle is greater than a vehicle length and if the distance is determined greater than the vehicle length, detect at least one non-OBU-equipped vehicle stopped in the vehicle queue behind the first OBU-equipped vehicle.

Abstract: A connected traffic safety system comprises at least one roadside unit and a vehicle detection device. The roadside unit is configured to transmit wireless signals and receive corresponding responses from a corresponding wireless device of an Onboard Unit (OBU)-equipped vehicle, and to send at least one of vehicle location data, direction heading data, elevation data and speed data from the OBU-equipped vehicle to a traffic signal controller. The vehicle detection device is configured to generate vehicle detection data of at least one non-Onboard Unit (OBU)-equipped vehicle. The roadside unit to receive the vehicle detection data for creating a proxy data wireless message for the non-Onboard Unit (OBU)-equipped vehicle. The roadside unit to mimic the proxy data wireless message as a first Basic Safety Message (BSM) for the non-Onboard Unit (OBU)-equipped vehicle that would have been available to the Onboard Unit (OBU)-equipped vehicle present nearby.

Abstract: A field tile float is slidably mounted on a trash guard to prevent crop residue trash from clogging the drain inlet of field tile. The float includes a buoyant body extending around the trash guard cage and a guide frame slidably mounted on the trash guard post. When surface water in the field rises, the float is lifted to allow water to flow beneath the float into the drain pipe, while the buoyant body blocks field trash from clogging the trash guard.

Abstract: A corrugated plastic sheet is provided having two outer sheets and internal ribs. The ratio of the thicknesses between each outer sheet and a rib is approximately 2:1. This ratio can be adjusted so that the upper and lower sheets are thicker than the thickness of the ribs. The die itself does not need to be changed. Rather, the die settings are merely adjusted.

Abstract: Embodiments include an emergency traffic management system includes a traffic management center configured to receive traffic information, a traffic signal controller in communication with the traffic management center, and roadside equipment in communication with the traffic signal controller. The roadside equipment includes a transceiver configured to communicate with one or more mobile devices via a wireless communications channel, a memory device configured to store emergency information, a processor configured to provide the emergency information to the one or more mobile devices via the transceiver, and a power supply configured to provide power to the processor and the transceiver.

Abstract: A roadside equipment (RSE) system that can be used for controlling traffic signals and other equipment and corresponding method. A method includes maintaining a traffic signal in a GREEN phase in a first direction, including displaying a GREEN indicator in the first direction. The method includes detecting an event indicating that the phase of the traffic signal in the first direction should be changed and changing the phase of the traffic signal to a pre-YELLOW phase in response to the detected event, including continuing to display the GREEN indicator in the first direction. The method includes wirelessly transmitting a message indicating the pre-YELLOW phase and a phase time, the phase time indicating a next change of phase of the traffic signal, and changing the phase of the traffic signal to a YELLOW phase when the phase time has been reached.

Abstract: A computer implemented method, apparatus, and computer usable program code for executing a process within a virtual machine. A module is injected into an operating system for the virtual machine to form an injected module. The injected module is executed to load an agent process within an application space within the virtual machine. Execution of the agent process is initiated by the injected module.