Abstract: This disclosure provides various TcBuster transposases and transposons, systems, and methods of use.

Abstract: This disclosure provides various TcBuster transposases and transposons, systems, and methods of use.

Abstract: A method for detecting aggressive tumor behavior and/or increased risk for tumor metastasis generally includes analyzing a tumor sample from the subject for expression of transcripts from coding regions of the cell cycle gene cluster, the immune-1 gene cluster, or the immune-2 gene cluster; computing a sum of log2-transformed mean-centered expression values, thereby generating a Gene Cluster Expression Summary Score (GCESS) for the sample; and detecting a tumor with aggressive behavior and/or increased risk for tumor metastasis. An aggressive tumor and/or increased risk for tumor metastasis may be indicated where the cell cycle gene cluster is analyzed and the sample GCESS is greater than 0, or the immune-1 gene cluster or the immune-2 gene cluster is analyzed and the sample GCESS is less than 0.

Abstract: A conditional rescue system generally includes a gene transfer system, a polynucleotide including a nuclease-resistant target coding region, and a coding region encoding a conditionally-lethal polypeptide. The gene transfer system is effective to integrate into host cell DNA. The polynucleotide including the nuclease-resistant target coding region is under transcriptional control of an inducible promoter. The coding region encoding a conditionally-lethal polypeptide is transcriptionally linked to the target coding region.

Abstract: Genetically modified compositions, such as non-viral vectors and T cells, for treating cancer are disclosed. Also disclosed are the methods of making and using the genetically modified compositions in treating cancer.

Abstract: Genetically modified compositions, such as non-viral vectors and T cells, for treating cancer are disclosed. Also disclosed are the methods of making and using the genetically modified compositions in treating cancer.

Abstract: Methods of using a transposon as an insertional mutagen are provided. Also provided is a transgenic animal that includes polynucleotides encoding a transposon and transposase that can be used to identify genomic sequences. The methods and transgenic animals may be used to detect cancer-related genes by identifying common insertion sites in tumor cells.

Abstract: Disclosed herein is a method for producing antibodies against an antigen of interest. Animal cells are exposed to both the antigen of interest and a recombinant retroviral vector. The vector contains a combination of oncogenes capable of inducing plasmacytomas. Plasmacytoma formation takes place rapidly and takes place in the presence of the antigen. A very high proportion of the plasmacytomas that are recovered are antigen specific.

Abstract: Disclosed herein is a method for producing antibodies against an antigen of interest. Animal cells are exposed to both the antigen of interest and a recombinant retroviral vector. The vector contains a combination of oncogenes capable of inducing plasmacytomas. Plasmacytoma formation takes place rapidly and takes place in the presence of the antigen. A very high proportion of the plasmacytomas that are recovered are antigen specific.