Patents by Inventor David M. Lynn
David M. Lynn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8834918Abstract: A composition for delivery of a molecule into a cell is provided. The composition includes a protein transduction domain that is conjugated to the molecule which is incorporated into a multilayered film. Preferably, the protein transduction domain is a cationic protein transduction domain. More preferably, the cationic protein transduction domain is nonaarginine, and the multilayered film includes polyelectrolyte multilayers. When the composition is presented to a cell, the multilayered film dissolves or erodes in physiological media, and the molecule is delivered into the cell.Type: GrantFiled: January 22, 2008Date of Patent: September 16, 2014Assignee: Wisconsin Alumni Research FoundationInventors: David M. Lynn, Ronald T. Raines, Christopher M. Jewell, Stephen M. Fuchs, Ryan M. Flessner
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Patent number: 8734851Abstract: The present invention provides implantable medical devices coated with polyelectrolyte assemblies that are fabricated by layer-by-layer deposition of nucleic acid and polycation. Such devices facilitate the local delivery of a nucleic acid contained in the polyelectrolyte assembly into a cell or tissue at an implantation site. Also provided are methods of fabricating and using implantable medical devices according to the invention.Type: GrantFiled: May 1, 2006Date of Patent: May 27, 2014Assignee: Wisconsin Alumni Research FoundationInventors: David M. Lynn, Jingtao Zhang, Christopher M. Jewell, Nathaniel J. Fredin
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Patent number: 8716422Abstract: Materials and Methods for the generation of polyelectrolyte multilayers that can erode to release cationic components. The multilayers comprise layers that contain one or more cations and one or more charge-dynamic anionic polymers. Charge-dynamic anionic polymers contain side chains having removable functional groups. Removal of the functional groups results in a change in the net change in the charge of the polymer which can disrupt interactions between cations and the anionic polymers and facilitate release of cations.Type: GrantFiled: October 23, 2012Date of Patent: May 6, 2014Assignee: Wisconsin Alumni Research FoundationInventors: Xianghui Liu, Jingtao Zhang, David M. Lynn
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Publication number: 20140094399Abstract: Poly(?-amino esters) prepared from the conjugate addition of bis(secondary amines) or primary amines to a bis(acrylate ester) are described. Methods of preparing these polymers from commercially available starting materials are also provided. These tertiary amine-containing polymers are preferably biodegradable and biocompatible and may be used in a variety of drug delivery systems. Given the poly(amine) nature of these polymers, they are particularly suited for the delivery of polynucleotides. Nanoparticles containing polymer/polynucleotide complexes have been prepared. The inventive polymers may also be used to encapsulate other agents to be delivered. They are particularly useful in delivering labile agents given their ability to buffer the pH of their surroundings.Type: ApplicationFiled: September 17, 2013Publication date: April 3, 2014Applicant: Massachusetts Institute of TechnologyInventors: Robert S. Langer, David M. Lynn, David A. Putnam, Mansoor M. Amiji, Daniel Griffith Anderson
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Publication number: 20130344563Abstract: A class of anionic oligomers and polymers that function for inhibition of nucleases, particularly RNase. Specific inhibitors include mixtures of oligomers of vinyl sulfate. Methods for inhibition or inactivation of one or more nucleases in vitro which comprises the step of contacting the one or more nucleases in a biological medium with one or more of the anionic oligomeric or polymeric inhibitors of this invention. Kits for carrying out a biological procedure, biological reaction and/or a biological assay containing one or more inhibitors of this invention. The use of oligomers and/or polymers of this invention as additives in buffers or reagents. The inhibitors of the invention can also be attached to surfaces to provide for removal of nucleases from media, solutions or other liquids in contact with the solid.Type: ApplicationFiled: June 10, 2013Publication date: December 26, 2013Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Ronald T. RAINES, Bryan D. Smith, Matthew B. Soellner, David M. Lynn
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Patent number: 8574420Abstract: Multilayered films, particularly ultrathin multilayered films comprising cationic polymers which are useful for controlled release of anionic species, particularly for controlled release of nucleic acids. The multilayer films herein are useful for temporal controlled released of anionic species, particularly one or more anionic peptides, proteins, nucleic acids or other anionic biological agents. In one aspect, the invention relates to multilayer films which release anionic species (anions) with separate and/or distinct release profiles, particularly wherein the anions are one or more anionic peptides, proteins or nucleic acids or other anionic biological agents.Type: GrantFiled: November 21, 2011Date of Patent: November 5, 2013Assignee: Wisconsin Alumni Research FoundationInventors: David M. Lynn, Jingtao Zhang, Xianghui Liu
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Patent number: 8557231Abstract: Poly(?-amino esters) prepared from the conjugate addition of bis(secondary amines) or primary amines to a bis(acrylate ester) are described. Methods of preparing these polymers from commercially available starting materials are also provided. These tertiary amine-containing polymers are preferably biodegradable and biocompatible and may be used in a variety of drug delivery systems. Given the poly(amine) nature of these polymers, they are particularly suited for the delivery of polynucleotides. Nanoparticles containing polymer/polynucleotide complexes have been prepared. The inventive polymers may also be used to encapsulate other agents to be delivered. They are particularly useful in delivering labile agents given their ability to buffer the pH of their surroundings.Type: GrantFiled: April 6, 2005Date of Patent: October 15, 2013Assignee: Massachusetts Institute of TechnologyInventors: Robert S. Langer, David M. Lynn, David Putnam, Mansoor M. Amiji, Daniel G. Anderson
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Patent number: 8524368Abstract: The present invention provides dynamic charge state cationic polymers that are useful for delivery of anionic molecules. The dynamic charge state cationic polymers are designed to have cationic charge densities that decrease by removal of removable functional groups from the polymers. The present invention also provides interpolyelectrolyte complexes containing the polymers complexed to a polyanion. Methods for using the interpolyelectrolyte complexes to deliver anionic compounds are also provided.Type: GrantFiled: December 14, 2011Date of Patent: September 3, 2013Assignee: Wisconsin Alumni Research FoundationInventors: David M. Lynn, Adam D. Miller
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Patent number: 8460684Abstract: A class of anionic oligomers and polymers that function for inhibition of nucleases, particularly RNase. Specific inhibitors include mixtures of oligomers of vinyl sulfate. Methods for inhibition or inactivation of one or more nucleases in vitro which comprises the step of contacting the one or more nucleases in a biological medium with one or more of the anionic oligomeric or polymeric inhibitors of this invention. Kits for carrying out a biological procedure, biological reaction and/or a biological assay containing one or more inhibitors of this invention. The use of oligomers and/or polymers of this invention as additives in buffers or reagents. The inhibitors of the invention can also be attached to surfaces to provide for removal of nucleases from media, solutions or other liquids in contact with the solid.Type: GrantFiled: March 18, 2005Date of Patent: June 11, 2013Assignee: Wisconsin Alumni Research FoundationInventors: Ronald T. Raines, Bryan D. Smith, Matthew B. Soellner, David M. Lynn
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Publication number: 20130136782Abstract: Compounds described herein inhibit biofilm formation or disperse pre-formed biofilms of Gram-negative bacteria. Biofilm-inhibitory compounds can be encapsulated or contained in a polymer matrix for controlled release. Coatings, films, multilayer films, hydrogels, microspheres and nanospheres as well as pharmaceutical compositions and disinfecting compositions containing biofilm-inhibitory compounds are also provided. Methods for inhibiting formation of biofilms or dispersing already formed biofilms are provided. Methods for treating infections of gram-negative bacteria which form biofilms, particularly those of Pseudomonas and more particularly P. aeruginosa.Type: ApplicationFiled: November 5, 2012Publication date: May 30, 2013Inventors: Helen BLACKWELL, Reto Frei, Anthony Breitbach, David M. Lynn, Adam H. Broderick
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Publication number: 20130122055Abstract: Materials and Methods for the generation of polyelectrolyte multilayers that can erode to release cationic components. The multilayers comprise layers that contain one or more cations and one or more charge-dynamic anionic polymers. Charge-dynamic anionic polymers contain side chains having removable functional groups. Removal of the functional groups results in a change in the net change in the charge of the polymer which can disrupt interactions between cations and the anionic polymers and facilitate release of cations.Type: ApplicationFiled: October 23, 2012Publication date: May 16, 2013Inventors: Xianghui LIU, Jingtao Zhang, David M. Lynn
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Patent number: 8324333Abstract: Materials and methods for the generation of polyelectrolyte multilayers that can erode to release cationic components. The multilayers comprise layers that contain one or more cations and one or more charge-dynamic anionic polymers. Charge-dynamic anionic polymers contain side chains having removable functional groups. Removal of the functional groups results in a change in the net change in the charge of the polymer which can disrupt interactions between cations and the anionic polymers and facilitate release of cations.Type: GrantFiled: June 5, 2009Date of Patent: December 4, 2012Assignee: Wisconsin Alumni Research FoundationInventors: Xianghui Liu, Jingtao Zhang, David M. Lynn
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Patent number: 8287849Abstract: Poly(?-amino esters) prepared from the conjugate addition of bis(secondary amines) or primary amines to a bis(acrylate ester) are described. Methods of preparing these polymers from commercially available starting materials are also provided. These tertiary amine-containing polymers are preferably biodegradable and biocompatible and may be used in a variety of drug delivery systems. Given the poly(amine) nature of these polymers, they are particularly suited for the delivery of polynucleotides. Nanoparticles containing polymer/polynucleotide complexes have been prepared. The inventive polymers may also be used to encapsulate other agents to be delivered. They are particularly useful in delivering labile agents given their ability to buffer the pH of their surroundings.Type: GrantFiled: July 23, 2009Date of Patent: October 16, 2012Assignee: Massachusetts Institute of TechnologyInventors: Robert S. Langer, David M. Lynn, David Putnam, Mansoor M. Amiji, Daniel G. Anderson
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Publication number: 20120134926Abstract: The present invention provides dynamic charge state cationic polymers that are useful for delivery of anionic molecules. The dynamic charge state cationic polymers are designed to have cationic charge densities that decrease by removal of removable functional groups from the polymers. The present invention also provides interpolyelectrolyte complexes containing the polymers complexed to a polyanion. Methods for using the interpolyelectrolyte complexes to deliver anionic compounds are also provided.Type: ApplicationFiled: December 14, 2011Publication date: May 31, 2012Applicant: Wisconsin Alumni Research FoundationInventors: David M. Lynn, Adam D. Miller
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Publication number: 20120065358Abstract: Poly(?-amino esters) prepared from the conjugate addition of bis(secondary amines) or primary amines to a bis(acrylate ester) are described. Methods of preparing these polymers from commercially available starting materials are also provided. These tertiary amine-containing polymers are preferably biodegradable and biocompatible and may be used in a variety of drug delivery systems. Given the poly(amine) nature of these polymers, they are particularly suited for the delivery of polynucleotides. Nanoparticles containing polymer/polynucleotide complexes have been prepared. The inventive polymers may also be used to encapsulate other agents to be delivered. They are particularly useful in delivering labile agents given their ability to buffer the pH of their surroundings.Type: ApplicationFiled: November 21, 2011Publication date: March 15, 2012Applicant: Massachusetts Institute of TechnologyInventors: Robert S. Langer, David M. Lynn, David A. Putnam, Mansoor M. Amiji, Daniel G. Anderson
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Publication number: 20120065616Abstract: Multilayered films, particularly ultrathin multilayered films comprising cationic polymers which are useful for controlled release of anionic species, particularly for controlled release of nucleic acids. The multilayer films herein are useful for temporal controlled released of anionic species, particularly one or more anionic peptides, proteins, nucleic acids or other anionic biological agents. In one aspect, the invention relates to multilayer films which release anionic species (anions) with separate and/or distinct release profiles, particularly wherein the anions are one or more anionic peptides, proteins or nucleic acids or other anionic biological agents.Type: ApplicationFiled: November 21, 2011Publication date: March 15, 2012Inventors: David M. Lynn, Jingtao Zhang, Xianghui Liu
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Patent number: 8105652Abstract: A decomposable thin film comprising a plurality of polyelectrolyte layers of alternating charge, wherein decomposition of the thin film is characterized by degradation of at least a portion of the polyelectrolyte layers.Type: GrantFiled: July 26, 2006Date of Patent: January 31, 2012Assignee: Massachusetts Institute of TechnologyInventors: Kris C. Wood, Helen F. Chuang, Robert D. Batten, David M. Lynn, Paula T. Hammond Cunningham
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Patent number: 8097277Abstract: The present invention provides dynamic charge state cationic polymers that are useful for delivery of anionic molecules. The dynamic charge state cationic polymers are designed to have cationic charge densities that decrease by removal of removable functional groups from the polymers. The present invention also provides interpolyelectrolyte complexes containing the polymers complexed to a polyanion. Methods for using the interpolyelectrolyte complexes to deliver anionic compounds are also provided.Type: GrantFiled: November 19, 2010Date of Patent: January 17, 2012Assignee: Wisconsin Alumni Research FoundationInventors: David M. Lynn, Adam D. Miller
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Publication number: 20110306142Abstract: Devices and methods for immobilizing micrometer sized liquid domains onto a chemically functionalized substrate surface are disclosed. A multifunctional polymer is adsorbed at the surface interface of the liquid microdomains, and the liquid microdomains are immobilized by covalent bonding or non-covalent forces such as electrostatic attraction between the adsorbed multifunctional polymer and the functionalized substrate surface.Type: ApplicationFiled: April 15, 2011Publication date: December 15, 2011Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: David M. Lynn, Nicholas L. Abbott, Maren E. Buck, Michael I. Kinsinger
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Patent number: RE43612Abstract: Poly(?-amino esters) prepared from the conjugate addition of bis(secondary amines) or primary amines to a bis(acrylate ester) are described. Methods of preparing these polymers from commercially available starting materials are also provided. These tertiary amine-containing polymers are preferably biodegradable and biocompatible and may be used in a variety of drug delivery systems. Given the poly(amine) nature of these polymers, they are particularly suited for the delivery of polynucleotides. Nanoparticles containing polymer/polynucleotide complexes have been prepared. The inventive polymers may also be used to encapsulate other agents to be delivered. They are particularly useful in delivering labile agents given their ability to buffer the pH of their surroundings. A system for preparing and screening polymers in parallel using semi-automated robotic fluid delivery systems is also provided.Type: GrantFiled: September 28, 2009Date of Patent: August 28, 2012Assignee: Massachusetts Institute of TechnologyInventors: Daniel Griffith Anderson, David M. Lynn, Akin Akinc, Robert S. Langer