Abstract: A method of manufacturing an article is provided. The method includes feeding a polymeric material into an extruder including a nozzle, and feeding a continuous fiber into the extruder, the continuous fiber and the polymeric material together forming a molding compound. A three-dimensional preform is formed by discharging the molding compound from the nozzle onto a deposition surface. A mold charge is formed by positioning the three-dimensional preform within a mold that includes a top mold component and a bottom mold component. The mold charge is compression molded within the mold to form a finished article.

Abstract: Compositions and methods for genetically modifying at least one nucleic acid sequence of interest in a polyploid organism.

Abstract: Certain embodiments provide a method for preparing a biochemical product (e.g., phenol, catechol, or muconic acid, or a salt thereof). For example, such methods include contacting a recombinant host having two or more recombinant pathways with a fermentable carbon source and growing the recombinant cell for a time sufficient to synthesize the product. In certain embodiments, each recombinant pathway: 1) is capable of producing the same final biochemical product; 2) comprises at least one gene encoding a polypeptide; 3) is derived from a different endogenous metabolite as its immediate precursor; and 4) converges to the same final product or the same intermediate metabolite.

Abstract: Described herein are engineered metabolic pathways in recombinant microorganism host cells which result in the production of 2-phenylethanol or 2-phenylacetic acid. Also described herein are methods of using the recombinant microorganisms for the production of 2-phenylethanol or 2-phenylacetic acid.

Abstract: Described herein are engineered metabolic pathways in recombinant microorganism host cells which result in the production of 2-phenylethanol or 2-phenylacetic acid. Also described herein are methods of using the recombinant microorganisms for the production of 2-phenylethanol or 2-phenylacetic acid.

Abstract: A system and method for mining social media signals and cues i) created by a user (for example, a customer) and/or ii) to which the user is exposed (the “data”), and for processing that data as it relates to a service (including a fee or subscription-based service), in order to predict the user's predisposition or likelihood to either leave the subscription or the service or reduce his/her engagement with the subscription or the service.

Abstract: Systems, apparatus, and methods for conducting amplification-based analyses, including PCR testing. In one illustrative embodiment, a system may include a testing container assembly and a testing unit. The testing container assembly may include a sample collection port, a sample preparation chamber, and a reaction chamber. The sample collection port may include a bottom opening sealed by a plug member. In use, the testing container assembly may be placed in a seat of the testing unit with a sample in the sample collection port, closed by a lid. A plunger may dislodge the plug member and the sample drawn into the sample preparation chamber. Once sample preparation is complete, a channel may be opened, and the prepared sample flows into the reaction chamber which is then sealed. Testing including amplification reactions, may then be performed, followed by detection, as by detecting fluorescent emissions in the reaction chamber.

Abstract: A method of forming an acid-doped polyaniline (emeraldine salt) (PANi-ES) solution including steps of: (i) mixing polyaniline (emeraldine base) (PANi-EB) with a PANi-EB solvent and a gel-inhibitor to form a gel-inhibited PANi-EB solution; (ii) removing the gel-inhibitor from the gel-inhibited PANi-EB solution to form a PANi-EB solution; and (iii) adding an acid dopant to the PANi-EB solution to form a PANi-ES solution.

Abstract: A system and method for mining social media signals and cues i) created by a user (for example, a customer) and/or ii) to which the user is exposed (the “data”), and for processing that data as it relates to a service (including a fee or subscription-based service), in order to predict the user's predisposition or likelihood to either leave the subscription or the service or reduce his/her engagement with the subscription or the service.

Abstract: Compositions and methods for genetically modifying at least one nucleic acid sequence of interest in a polyploid organism.

Abstract: A method for the in vivo production of 1,3-butadiene from 2,4-pentadienoate is described (FIG. 1). Enzymes capable of decarboxylating 2,4-pentadienoate to 1,3-butadiene have been discovered. Recombinant expression of these newly discovered enzymes has resulted in the engineering of microorganisms capable of producing 1,3-butadiene when cultured in the presence of 2,4-pentadienoate. 1,3-butadienoate is an important monomer used in the manufacturing of rubbers and plastics. This invention will help to enable the biological production of 1,3-butadiene from renewable resources such as sugar, for example.

Abstract: A system and method for mining social media signals and cues i) created by a user (for example, a customer) and/or ii) to which the user is exposed (the “data”), and for processing that data as it relates to a service (including a fee or subscription-based service), in order to predict the user's predisposition or likelihood to either leave the subscription or the service or reduce his/her engagement with the subscription or the service.

Abstract: Described herein are engineered metabolic pathways in recombinant microorganism host cells which result in the production of 2-phenylethanol or 2-phenylacetic acid. Also described herein are methods of using the recombinant microorganisms for the production of 2-phenylethanol or 2-phenylacetic acid.

Abstract: A method of forming an acid-doped polyaniline (emeraldine salt) (PANi-ES) solution including steps of: (i) mixing polyaniline (emeraldine base) (PANi-EB) with a PANi-EB solvent and a gel-inhibitor to form a gel-inhibited PANi-EB solution; (ii) removing the gel-inhibitor from the gel-inhibited PANi-EB solution to form a PANi-EB solution; and (iii) adding an acid dopant to the PANi-EB solution to form a PANi-ES solution.

Abstract: Certain embodiments provide a method for preparing a biochemical product (e.g., phenol, catechol, or muconic acid, or a salt thereof). For example, such methods include contacting a recombinant host having two or more recombinant pathways with a fermentable carbon source and growing the recombinant cell for a time sufficient to synthesize the product. In certain embodiments, each recombinant pathway: 1) is capable of producing the same final biochemical product; 2) comprises at least one gene encoding a polypeptide; 3) is derived from a different endogenous metabolite as its immediate precursor; and 4) converges to the same final product or the same intermediate metabolite.

Abstract: A method includes electronically gathering, from at least one independent data source, a set of data points, wherein the data points comprise information about the behavior of a population of mobile-device customers and potential mobile-device customers. A numerical score for the mobile-device customer or potential mobile-device customer is determined based on the information. The determined numerical score is compared to one or more predetermined numerical thresholds, and based on the comparing, the mobile-device customer's or potential mobile-device customer's likelihood to churn and whether the mobile-device customer or potential mobile-device customer should be the target of emphasized marketing efforts is determined.

Abstract: A method for the in vivo production of 1,3-butadiene from 2,4-pentadienoate is described (FIG. 1). Enzymes capable of decarboxylating 2,4-pentadienoate to 1,3-butadiene have been discovered. Recombinant expression of these newly discovered enzymes has resulted in the engineering of microorganisms capable of producing 1,3-butadiene when cultured in the presence of 2,4-pentadienoate. 1,3-butadienoate is an important monomer used in the manufacturing of rubbers and plastics. This invention will help to enable the biological production of 1,3-butadiene from renewable resources such as sugar, for example.

Abstract: Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.