Abstract: Novel methods for the in vivo production of phenol from renewable substrates using a recombinant microorganism (FIG. 1). Additionally, methods for the in vivo production of catechol and cis,cis-muconic acid from renewable substrates using a recombinant microorganism are disclosed. A host cell expresses at least one gene encoding a polypeptide that possesses isochorismate synthase activity, at least one gene encoding a polypeptide that possesses isochorismate pyruvate lyase activity, and at least one gene encoding a polypeptide that possesses salicylic acid decarboxylase activity. In the case of catechol, the host cell must additionally express at least one gene encoding a polypeptide that possesses phenol 2-monooxygenase activity. In the case of cis,cis-muconic acid, the host cell must additionally express at least one gene encoding a polypeptide that possesses phenol 2-monooxygenase activity and at least one gene encoding a polypeptide that possesses catechol-1,2-dioxygenase activity.

Abstract: A method for the in vivo production of 1,3-butadiene from 2,4-pentadienoate is described (FIG. 1). Enzymes capable of decarboxylating 2,4-pentadienoate to 1,3-butadiene have been discovered. Recombinant expression of these newly discovered enzymes has resulted in the engineering of microorganisms capable of producing 1,3-butadiene when cultured in the presence of 2,4-pentadienoate. 1,3-butadienoate is an important monomer used in the manufacturing of rubbers and plastics. This invention will help to enable the biological production of 1,3-butadiene from renewable resources such as sugar, for example.

Abstract: Construction and expression of synthetic pathways to produce (S) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.

Abstract: A social media identity/construct is created for a person by locating social media profiles of the person at least by one or both of i) recognizing image(s) in one or more social media postings related to the person and ii) correlating geolocation data embedded in one or more social media postings related to the person or the person's social media connections, analyzing, monitor social media outlets and channels to create a social media profile for the person.

Abstract: Construction and expression of synthetic pathways to produce (5) or (R)-3-hydroxybutyrate (3HB) as enantiomerically-pure products by genetically engineering cyanobacterium Synechocystis sp. PCC 6803. Under optimized growth conditions, the pathway employing phaA and phaB from R. eutropha was the most effective, producing up to 533.4±5.5 mg/l (R)-3HB after 21 days photosynthetic cultivation. For the first time, the feasibility and high efficiency of producing 3HB using solar energy and CO2 as sole energy and carbon sources by engineered cyanobacteria is demonstrated.

Abstract: A lifter system includes a harness, at least one lifting strap carried by a front, central buckle on the harness, and two arm loops connected to the central buckle or to the harness near the central buckle. The arm loops are used to supply supplemental or substitute lifting force during lifting or maneuvering a heavy or bulky object. Preferably, a set of two shoulder harnesses is used by two persons standing face-to-face with a single lifter strap between them threaded through both of the central buckles, wherein the two persons wear the arm loops on their forearms and raise their arms to lift the central buckles, and hence, to lift the lifting strap, to levels higher than may be possible with only force supplied by the harnesses.

Abstract: A lifter system includes a harness, at least one lifting strap carried by a front, central buckle on the harness, and two arm loops connected to the central buckle or to the harness near the central buckle. The arm loops are used to supply supplemental or substitute lifting force during lifting or maneuvering a heavy or bulky object. Preferably, a set of two shoulder harnesses is used by two persons standing face-to-face with a single lifter strap between them threaded through both of the central buckles, wherein the two persons wear the arm loops on their forearms and raise their arms to lift the central buckles, and hence, to lift the lifting strap, to levels higher than may be possible with only force supplied by the harnesses.

Abstract: A strap lifter for use with a harness is shown and described. The harness may have two shoulder straps that support a central, front buckle for use at about the lower, central belly of a user. A generally horizontal buckle strap has a fixed, rigid lower loop beneath a movable, flexible upper loop secured to the front of the buckle strap. The lower and upper loops are generally parallel, and close, to each other. Typically, the distance between the two loops is slightly more than the thickness of a lifter strap which is threaded between the two loops. Preferably, a set of two harnesses is used by two persons standing face-to-face with a single lifter strap between them threaded through both of the buckles.

Abstract: A method for determining and/or engineering photosynthetic mutant algal strains comprising: (A) pre-screening wild-type or parent strains to select for photosynthetic efficiency; (B) cause genetic mutations in the group of wild-type and/or parent strains from pre-screening (A) to form genetic mutant strains; (C) screening the genetic mutant strains for photosynthetic efficiency in mass cultures; and (D) further screening the genetic mutants resulting from screening (C) by measuring biomass productivity to select strains having relatively high biomass/lipid/starch productivity.

Abstract: A system and method for mining social media signals and cues i) created by a user (for example, a customer) and/or ii) to which the user is exposed (the “data”), and for processing that data as it relates to a service (including a fee or subscription-based service), in order to predict the user's predisposition or likelihood to either leave the subscription or the service or reduce his/her engagement with the subscription or the service.

Abstract: A method for the in vivo production of 1,3-butadiene from 2,4-pentadienoate is described (FIG. 1). Enzymes capable of decarboxylating 2,4-pentadienoate to 1,3-butadiene have been discovered. Recombinant expression of these newly discovered enzymes has resulted in the engineering of microorganisms capable of producing 1,3-butadiene when cultured in the presence of 2,4-pentadienoate. 1,3-butadienoate is an important monomer used in the manufacturing of rubbers and plastics. This invention will help to enable the biological production of 1,3-butadiene from renewable resources such as sugar, for example.

Abstract: A strap lifter for use with a harness is shown and described. The harness may have two shoulder straps that support a central, front buckle for use at about the lower, central belly of a user. A generally horizontal buckle strap has a fixed, rigid lower loop beneath a movable, flexible upper loop secured to the front of the buckle strap. The lower and upper loops are generally parallel, and close, to each other. Typically, the distance between the two loops is slightly more than the thickness of a lifter strap which is threaded between the two loops. Preferably, a set of two harnesses is used by two persons standing face-to-face with a single lifter strap between them threaded through both of the buckles.

Abstract: Novel methods for the in vivo production of phenol from renewable substrates using a recombinant microorganism (FIG. 1). Additionally, methods for the in vivo production of catechol and cis,cis-muconic acid from renewable substrates using a recombinant microorganism are disclosed. A host cell expresses at least one gene encoding a polypeptide that possesses isochorismate synthase activity, at least one gene encoding a polypeptide that possesses isochorismate pyruvate lyase activity, and at least one gene encoding a polypeptide that possesses salicylic acid decarboxylase activity. In the case of catechol, the host cell must additionally express at least one gene encoding a polypeptide that possesses phenol 2-monooxy-genase activity. In the case of cis,cis-muconic acid, the host cell must additionally express at least one gene encoding a polypeptide that possesses phenol 2-monooxygenase activity and at least one gene encoding a polypeptide that possesses catechol-1,2-dioxygenase activity.

Abstract: A social media identity/construct is created for a person by locating social media profiles of the person at least by one or both of i) recognizing image(s) in one or more social media postings related to the person and ii) correlating geolocation data embedded in one or more social media postings related to the person or the person's social media connections, analyzing, monitor social media outlets and channels to create a social media profile for the person.

Abstract: A strap lifter for use with a harness is shown and described. The harness may have two shoulder straps that support a central, front buckle for use at about the lower, central belly of a user. A generally horizontal buckle strap has a fixed, rigid lower loop beneath a movable, flexible upper loop secured to the front of the buckle strap. The lower and upper loops are generally parallel, and close, to each other. Typically, the distance between the two loops is slightly more than the thickness of a lifter strap which is threaded between the two loops. Preferably, a set of two harnesses is used by two persons standing face-to-face with a single lifter strap between them threaded through both of the buckles.

Abstract: A web crawler for downloading and analyzing the contents of a merchant's website. The web crawler may analyze the products advertised and determine whether a service plan is properly associated. The crawler may also analyze the placement of the service plans on the website, and store the information in a database. A dynamic mapper is also provided which can determine what service plan should be associated with a particular product. The dynamic mapper may also suggest what type of control to use for a particular customer. A webserver containing software for updating a webpage is also disclosed. A process for updating a webpage is also disclosed.

Abstract: A strap lifter for use with a harness is shown and described. The harness may have two shoulder straps that support a central, front buckle for use at about the lower, central belly of a user. A generally horizontal buckle strap has a fixed, rigid lower loop beneath a movable, flexible upper loop secured to the front of the buckle strap. The lower and upper loops are generally parallel, and close, to each other. Typically, the distance between the two loops is slightly more than the thickness of a lifter strap which is threaded between the two loops. Preferably, a set of two harnesses is used by two persons standing face-to-face with a single lifter strap between them threaded through both of the buckles.

Abstract: A method for determining and/or engineering photosynthetic mutant algal strains comprising: (A) pre-screening wild-type or parent strains to select for photosynthetic efficiency; (B) cause genetic mutations in the group of wild-type and/or parent strains from pre-screening (A) to form genetic mutant strains; (C) screening the genetic mutant strains for photosynthetic efficiency in mass cultures; and (D) further screening the genetic mutants resulting from screening (C) by measuring biomass productivity to select strains having relatively high biomass/lipid/starch productivity.

Abstract: A strap lifter for use with a harness is shown and described. The harness may have two shoulder straps that support a central, front buckle for use at about the lower, central belly of a user. A generally horizontal buckle strap has a fixed, rigid lower loop beneath a movable, flexible upper loop secured to the front of the buckle strap. The lower and upper loops are generally parallel, and close, to each other. Typically, the distance between the two loops is slightly more than the thickness of a lifter strap which is threaded between the two loops. Preferably, a set of two harnesses is used by two persons standing face-to-face with a single lifter strap between them threaded through both of the buckles.