Abstract: The present invention provides methods of generating a mammalian cell that is homozygous at a locus of interest, as well as cells made by the method. The present invention further provides methods of using the cells.

Abstract: This invention provides methods for obtaining targeted gene modification in vertebrate cells using parvoviral vectors, including adeno-associated virus (AAV). The parvoviral vectors used in the methods of the invention are capable of targeting a specific genetic modification to a preselected target locus in a cellular genome by homologous pairing.

Abstract: The present invention provides methods of generating a mammalian cell that is homozygous at a locus of interest, as well as cells made by the method. The present invention further provides methods of using the cells.

Abstract: This invention provides methods for obtaining targeted gene modification in vertebrate cells using parvoviral vectors, including adeno-associated virus (AAV). The parvoviral vectors used in the methods of the invention are capable of targeting a specific genetic modification to a preselected target locus in a cellular genome by homologous pairing.

Abstract: This invention provides methods for obtaining targeted gene modification in vertebrate cells using parvoviral vectors, including adeno-associated virus (AAV). The parvoviral vectors used in the methods of the invention are capable of targeting a specific genetic modification to a preselected target locus in a cellular genome by homologous pairing.

Abstract: A window unit installation safety device is disclosed for supporting a window unit in place in a rough opening during window unit installation. The window unit installation device is securable onto the window unit and is configured to safely and retentively support the window unit in a framed rough opening, such as a framed sidewall of a residential or commercial building. The window unit installation device includes a plurality of spaced legs configured to be received over a window nail fin.

Abstract: The present invention provides methods of generating a mammalian cell that is homozygous at a locus of interest, as well as cells made by the method. The present invention further provides methods of using the cells.

Abstract: A method and system for archiving and restoring data in a portable communication device. A first wireless communication connection is established between the communication device and a remote network capable of storing portable communication device data. The identity of an archival function to be performed is transmitted over the first communication connection. The indicated archival function is performed by transmitting data to or from the communication device. The function can be performed over the first wireless communication connection or a second wireless communication connection can be established for transmitting the information. The data can be converted by an upload converter into a format that is independent of the identity of the communication device. The converted data can be sent to other communication devices and reformatted for use by those devices. The first communication connection can be established by a communication device user or automatically.

Abstract: A method and system for archiving and restoring data in a portable communication device. A first wireless communication connection is established between the communication device and a remote network capable of storing portable communication device data. The identity of an archival function to be performed is transmitted over the first communication connection. The indicated archival function is performed by transmitting data to or from the communication device. The function can be performed over the first wireless communication connection or a second wireless communication connection can be established for transmitting the information. The data can be converted by an upload converter into a format that is independent of the identity of the communication device. The converted data can be sent to other communication devices and reformatted for use by those devices. The first communication connection can be established by a communication device user or automatically.

Abstract: The present invention comprises a system for communicating image data and other data. The system comprises a JPEG file having a JPEG data, at least one non-JPEG data, and a computer. The computer is programmed to read a predetermined number of sequential bytes of the non-JPEG data. The computer is also programmed to determine position of each byte in the sequential bytes that contain a JPEG marker and determine a locator byte, which is capable of indicating the position of any bytes in the sequential bytes that contain a JPEG marker. The computer is further programmed to write the locator byte in the JPEG file and write encoded bytes in the JPEG file. The computer writes the encoded bytes in the JPEG file in a manner wherein, for each byte in the sequential bytes, if the byte does not contain a JPEG marker, each byte is replaced with a bit, preferably written as 0, in the order of the byte's occurrence in the sequential bytes.

Abstract: An associative search methodology is presented whereby words, phrases, entire documents or technical jargon are input to the system for the purposes of searching a database for like items. A self-organizing associative data structure utilizing Latent Semantic Analysis (LSA) combined with a Hierarchical Mixture of Experts (HME) neural network learning system tracks relationships between words and word groupings as well as user significance feedback to automatically expand the search so that data elements can be found which cross technological/taxonomic boundaries. This results in an increase in both recall and precision parameters for each individual user's search.

Abstract: The invention provides methods and compositions relating to cholesterol 25-hydroxylase polypeptides having cholesterol 25-hydroxylase-specific structure and activity, related polynucleotides and modulators of cholesterol 25-hydroxylase function and serum cholesterol. The invention provides isolated cholesterol 25-hydroxylase hybridization probes and primers capable of specifically hybridizing with natural cholesterol 25-hydroxylase genes, cholesterol 25-hydroxylase-specific binding agents such as specific antibodies, agonists and antagonists, and methods of making and using the subject compositions in diagnosis (e.g. genetic hybridization screens for cholesterol 25-hydroxylase transcripts), therapy (e.g. cholesterol 25-hydroxylase inhibitors to modulate serum cholesterol) and in the biopharmaceutical industry (e.g. as immunogens, reagents for isolating natural 25-hydroxylase genes and transcripts, reagents for screening chemical libraries for lead pharmacological agents, etc.).

Abstract: The present invention comprises a system for communicating image data and other data. The system comprises a JPEG file having a JPEG data, at least one non-JPEG data, and a computer. The computer is programmed to read a predetermined number of sequential bytes of the non-JPEG data. The computer is also programmed to determine position of each byte in the sequential bytes that contain a JPEG marker and determine a locator byte, which is capable of indicating the position of any bytes in the sequential bytes that contain a JPEG marker. The computer is further programmed to write the locator byte in the JPEG file and write encoded bytes in the JPEG file. The computer writes the encoded bytes in the JPEG file in a manner wherein, for each byte in the sequential bytes, if the byte does not contain a JPEG marker, each byte is replaced with a bit, preferably written as 0, in the order of the byte's occurrence in the sequential bytes.

Abstract: The present invention provides isolated adenovirus-associated viruses ("AAV"), including AAV isolates designated AAV3B and AAV6. The invention also provides nucleic acid molecules of AAV3B (SEQ ID NO: 1) or AAV6 (SEQ ID NO: 2), including DNA or RNA, and provides substantially purified polypeptides encoded by AAV3B or AAV6, as well as antibodies specific for such polypeptides. The invention also provides infectious AAV3B and AAV6 clones; AAV viral vectors, which can be hybrid AAV viral vectors; AAV vector plasmids; and AAV helper plasmids; each comprising at least a portion of an AAV3B (SEQ ID NO: 1) or AAV6 (SEQ ID NO: 2) nucleic acid molecule. The invention further provides host cells containing at least a portion of an AAV3B or AAV6 nucleic acid molecule and progeny cells derived therefrom, and provides non-human transgenic mammals having an AAV vector genome stably integrated in a chromosome.

Abstract: Disclosed are diagnostic techniques for the detection of human prostate cancer. Genetic probes and methods useful in monitoring the progression and diagnosis of prostate cancer are described. The invention relates particularly to probes and methods for evaluating the presence of RNA species that are differentially expressed in prostate cancer compared to normal human prostate or benign prostatic hyperplasia.

Abstract: Disclosed are diagnostic techniques for the detection of human prostate cancer. Genetic probes and methods useful in monitoring the progression and diagnosis of prostate cancer are described. The invention relates particularly to probes and methods for evaluating the presence of RNA species that are differentially expressed in prostate cancer compared to normal human prostate or benign prostatic hyperplasia.

Abstract: The invention provides methods and compositions relating to neuronal PAS domain proteins (NPAS) and related nucleic acids. The proteins may be produced recombinantly from transformed host cells from the disclosed NPAS encoding nucleic acids or purified from human cells. The invention provides isolated NPAS hybridization probes and primers capable of specifically hybridizing with the disclosed NPAS gene, NPAS-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.

Abstract: This invention includes methods for increasing the efficiency of transduction of cells, including non-dividing cells, by recombinant AAV vectors. The methods utilize agents that alter certain aspects of DNA metabolism, more specifically, that affect DNA synthesis and/or affect repair, that impact on maintenance of chromosomal integrity, and/or that cause damage to the cellular DNA. Agents and vectors can now also be preselected and screened for transducing ability and/or transducing agents for their effect on DNA metabolism. These agents include tritiated nucleotides such as thymidine, gamma irradiation, UV irradiation, cis-platinum, etoposide, hydroxyurea and aphidicolin.

Abstract: The invention provides methods and compositions relating to endothelial PAS domain protein 1 (EPAS1) and related nucleic acids. The proteins may be produced recombinantly from transformed host cells from the disclosed EPAS1 encoding nucleic acids or purified from human cells. The invention provides isolated EPAS1 hybridization probes and primers capable of specifically hybridizing with the disclosed EPAS1 gene, EPAS1-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.

Abstract: Disclosed are methods and compositions for the preparation of steroid 5.alpha.-reductases by recombinant means, as well as for the use of these enzymes in screening assays for the identification of compounds which have the ability to inhibit or otherwise alter the enzymatic function of these enzymes. Biochemical and pharmacological evidence is presented to demonstrate the existence of more than one human steroid 5.alpha.-reductase. The DNA sequence encoding steroid 5.alpha.-reductase 2, the major active isozyme of human genital tissue, is disclosed herein, in addition to methods and compositions for its preparation and pharmacological analysis. The sequences disclosed herein may be used directly in the preparation of genetic constructs, or may be employed in the preparation of hybridization probes for the selection of enzyme-encoding sequences from other sources.