Patents by Inventor Der-Ing Liao

Der-Ing Liao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Ketol-acid reductoisomerase enzymes and methods of use
    Patent number: 9388392
    Abstract: Provided herein are polypeptides having ketol-acid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.
    Type: Grant
    Filed: September 14, 2015
    Date of Patent: July 12, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Sridhar Govindarajan, Yougen Li, Der-Ing Liao, Daniel P. O'Keefe, Jeremy Stephen Minshull, Steven Cary Rothman, Alexander Vincent Tobias
  • Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
    Patent number: 9284612
    Abstract: Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.
    Type: Grant
    Filed: February 22, 2011
    Date of Patent: March 15, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Der-Ing Liao, Mark J. Nelson, Michael G. Bramucci
  • Ketol-Acid Reductoisomerase Enzymes and Methods of Use
    Publication number: 20160032255
    Abstract: Provided herein are polypeptides having ketol-acid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.
    Type: Application
    Filed: September 14, 2015
    Publication date: February 4, 2016
    Inventors: Sridhar Govindarajan, Yougen Li, Der-Ing Liao, Daniel P. O'Keefe, Jeremy Stephen Minshull, Steven Cary Rothman, Alexander Vincent Tobias
  • Ketol-acid reductoisomerase using NADH
    Patent number: 9238801
    Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: January 19, 2016
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • Ketol-acid reductoisomerase enzymes and methods of use
    Patent number: 9169467
    Abstract: Provided herein are polypeptides having ketol-aid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.
    Type: Grant
    Filed: May 10, 2013
    Date of Patent: October 27, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Sridhar Govindarajan, Yougen Li, Der-Ing Liao, Daniel P. O'Keefe, Jeremy Stephen Minshull, Steven Cary Rothman, Alexander Vincent Tobias
  • KETOL-ACID REDUCTOISOMERASE USING NADH
    Publication number: 20150111269
    Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
    Type: Application
    Filed: December 23, 2014
    Publication date: April 23, 2015
    Inventors: Yougen LI, Der-Ing LIAO, Mark J. NELSON, Daniel P. OKEEFE
  • Ketol-acid reductoisomerase using NADH
    Patent number: 8945899
    Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
    Type: Grant
    Filed: December 15, 2009
    Date of Patent: February 3, 2015
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • Ketol-acid reductoisomerase using NADH
    Patent number: 8911981
    Abstract: Methods for the evolution of NADPH specific ketol-add reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: December 16, 2014
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • Ketol-acid reductoisomerase using NADH
    Patent number: 8895307
    Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
    Type: Grant
    Filed: July 16, 2012
    Date of Patent: November 25, 2014
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • KETOL-ACID REDUCTOISOMERASE USING NADH
    Publication number: 20140057329
    Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
    Type: Application
    Filed: March 15, 2013
    Publication date: February 27, 2014
    Applicant: Butamax(TM) Advanced Biofuels LLC
    Inventors: Youngen LI, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • KETOL-ACID REDUCTOISOMERASE ENZYMES AND METHODS OF USE
    Publication number: 20140051133
    Abstract: Provided herein are polypeptides having ketol-aid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.
    Type: Application
    Filed: May 10, 2013
    Publication date: February 20, 2014
    Applicant: BUTAMAX ADVANCED BIOFUELS, LLC
    Inventors: Sridhar Govindarajan, Yougen Li, Der-Ing Liao, Daniel P. O'Keefe, Jeremy Stephen Minshull, Steven Cary Rothman, Alexander Vincent Tobias
  • B-dependent dehydratases with improved reaction kinetics
    Patent number: 8569469
    Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
    Type: Grant
    Filed: August 16, 2010
    Date of Patent: October 29, 2013
    Assignee: E I du Pont de Nemours and Company
    Inventors: Katharine J. Gibson, Der-Ing Liao, Xiao-Song Tang
  • B-dependent dehydratases with improved reaction kinetics
    Patent number: 8445659
    Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
    Type: Grant
    Filed: August 16, 2010
    Date of Patent: May 21, 2013
    Assignee: E I du Pont de Nemours and Company
    Inventors: Katharine J. Gibson, Der-Ing Liao, Xiao-Song Tang
  • KETOL-ACID REDUCTOISOMERASE USING NADH
    Publication number: 20130072394
    Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
    Type: Application
    Filed: July 16, 2012
    Publication date: March 21, 2013
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • Ketol-acid reductoisomerase using NADH
    Patent number: 8222017
    Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
    Type: Grant
    Filed: November 15, 2011
    Date of Patent: July 17, 2012
    Assignee: Butamax(TM) Advanced Biofuels LLC
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe, Jean-Francois Tomb
  • Ketol-Acid Reductoisomerase Using NADH
    Publication number: 20120115197
    Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
    Type: Application
    Filed: November 15, 2011
    Publication date: May 10, 2012
    Inventors: YOUGEN LI, DER-ING LIAO, MARK J. NELSON, DANIEL P. OKEEFE
  • Ketol-acid reductoisomerase using NADH
    Patent number: 8129162
    Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
    Type: Grant
    Filed: December 18, 2008
    Date of Patent: March 6, 2012
    Assignee: Butamax(TM) Advanced Biofuels LLC
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • Mutant Delta-8 Desaturase Genes Engineered By Targeted Mutagensis and Their Use In Making Polyunsaturated Fatty Acids
    Publication number: 20110268864
    Abstract: The present invention relates to mutant delta-8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 omega-6, EDA] to dihomo-?-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 omega-3, ETrA] to eicosatetraenoic acid [20:3 omega-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant delta-8 desaturases in plants and oleaginous yeast are disclosed.
    Type: Application
    Filed: July 7, 2011
    Publication date: November 3, 2011
    Inventors: HOWARD GLENN DAMUDE, HONGXIAN HE, DER-ING LIAO, QUINN QUN ZHU
  • FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY ACTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES
    Publication number: 20110250610
    Abstract: Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.
    Type: Application
    Filed: February 22, 2011
    Publication date: October 13, 2011
    Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLC
    Inventors: DER-ING LIAO, MARK J. NELSON, MICHAEL G. BRAMUCCI
  • Mutant ?8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
    Patent number: 8026089
    Abstract: The present invention relates to mutant ?8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 ?-6, EDA] to dihomo-?-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 ?-3, ETrA] to eicosatetraenoic acid [20:3 ?-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant ?8 desaturases in plants and oleaginous yeast are disclosed.
    Type: Grant
    Filed: March 18, 2010
    Date of Patent: September 27, 2011
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Howard Glenn Damude, Hongxian He, Der-Ing Liao, Quinn Qun Zhu