Patents by Inventor Der-Ing Liao

Der-Ing Liao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Mutant delta-8 desaturase genes engineered by targeted mutagensis and their use in making polyunsaturated fatty acids
    Patent number: 8017838
    Abstract: The present invention relates to mutant delta-8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 omega-6, EDA] to dihomo-?-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 omega-3, ETrA] to eicosatetraenoic acid [20:3 omega-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant delta-8 desaturases in plants and oleaginous yeast are disclosed.
    Type: Grant
    Filed: December 6, 2007
    Date of Patent: September 13, 2011
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Howard G. Damude, Hongxian He, Der-ing Liao, Quinn Qun Zhu
  • B12 dependent dehydratases with improved reaction kinetics
    Patent number: 7985849
    Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
    Type: Grant
    Filed: March 19, 2008
    Date of Patent: July 26, 2011
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Katharine J. Gibson, Der-Ing Liao, Xiao-Song Tang
  • Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
    Patent number: 7910342
    Abstract: Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.
    Type: Grant
    Filed: April 16, 2008
    Date of Patent: March 22, 2011
    Assignee: Butamax(TM) Advanced Biofuels LLC
    Inventors: Der-Ing Liao, Mark J. Nelson, Michael G. Bramucci
  • B12-Dependent Dehydratases With Improved Reaction Kinetics
    Publication number: 20110021768
    Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
    Type: Application
    Filed: August 16, 2010
    Publication date: January 27, 2011
    Inventors: Katharine J. GIBSON, Der-Ing Liao, Xiao-Song Tang
  • B12-Dependent Dehydratases With Improved Reaction Kinetics
    Publication number: 20110021767
    Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
    Type: Application
    Filed: August 16, 2010
    Publication date: January 27, 2011
    Inventors: Katharine J. GIBSON, Der-Ing Liao, Xiao-Song Tang
  • MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENESIS AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
    Publication number: 20100297713
    Abstract: The present invention relates to mutant ?8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 ?-6, EDA] to dihomo-?-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 ?-3, ETrA] to eicosatetraenoic acid [20:3 ?-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant ?8 desaturases in plants and oleaginous yeast are disclosed.
    Type: Application
    Filed: March 18, 2010
    Publication date: November 25, 2010
    Applicant: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: HOWARD GLENN DAMUDE, Hongxian He, Der-ing Liao, Quinn Qun Zhu
  • KETOL-ACID REDUCTOISOMERASE USING NADH
    Publication number: 20100197519
    Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.
    Type: Application
    Filed: December 15, 2009
    Publication date: August 5, 2010
    Applicant: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • Mutant ?8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
    Patent number: 7709239
    Abstract: The present invention relates to mutant ?8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 ?-6, EDA] to dihomo-?-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 ?-3, ETrA] to eicosatetraenoic acid [20:3 ?-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant ?8 desaturases in plants and oleaginous yeast are disclosed.
    Type: Grant
    Filed: December 7, 2006
    Date of Patent: May 4, 2010
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Howard Glenn Damude, Hongxian He, Der-Ing Liao, Quinn Qun Zhu
  • MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
    Publication number: 20090176003
    Abstract: The present invention relates to mutant delta-8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 omega-6, EDA] to dihomo-?-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 omega-3, ETrA] to eicosatetraenoic acid [20:3 omega-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant delta-8 desaturases in plants and oleaginous yeast are disclosed.
    Type: Application
    Filed: December 6, 2007
    Publication date: July 9, 2009
    Inventors: Howard Glenn Damude, Hongxian He, Der-ing Liao, Quinn Qun Zhu
  • KETOL-ACID REDUCTOISOMERASE USING NADH
    Publication number: 20090163376
    Abstract: Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.
    Type: Application
    Filed: December 18, 2008
    Publication date: June 25, 2009
    Inventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
  • B12 DEPENDENT DEHYDRATASES WITH IMPROVED REACTION KINETICS
    Publication number: 20080293119
    Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
    Type: Application
    Filed: March 19, 2008
    Publication date: November 27, 2008
    Applicant: E.I. du Pont de Nemours and Company
    Inventors: Katharine GIBSON, Der-Ing LIAO, Xiao-Song TANG
  • FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY ACTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES
    Publication number: 20080261230
    Abstract: Methods for the fermentative production of isobutanol is provided by the fermentative growth of a recombinant microorganism expressing a highly active ketol-acid reductoisomerase enzyme in addition to other enzymes required for conversion of glucose to isobutanol.
    Type: Application
    Filed: April 16, 2008
    Publication date: October 23, 2008
    Inventors: DER-ING LIAO, Mark J. Nelson, Michael G. Bramucci
  • B-dependent dehydratases with improved reaction kinetics
    Patent number: 7410754
    Abstract: Sequences of B12-dependent dehydratases with improved reaction kinetics are presented. Use of these B12-dependent dehydratases reduce the rate of the enzyme's suicide inactivation in the presence of glycerol and 1,3-propanediol. The enzymes were created using error-prone PCR and oligonucleotide-directed mutagenesis to target the DhaB1 gene, which encodes the ?-subunit of glycerol dehydratase. Mutants with improved reaction kinetics were rapidly identified using high throughput assays.
    Type: Grant
    Filed: December 16, 2003
    Date of Patent: August 12, 2008
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Katharine J. Gibson, Der-Ing Liao, Xiao-Song Tang
  • Mutant delta-8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
    Publication number: 20080138868
    Abstract: The present invention relates to mutant ?8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 ?-6, EDA] to dihomo-?-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 ?-3, ETrA] to eicosatetraenoic acid [20:3 ?-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant ?8 desaturases in plants and oleaginous yeast are disclosed.
    Type: Application
    Filed: December 7, 2006
    Publication date: June 12, 2008
    Inventors: Howard Glenn Damude, Hongxian He, Der-Ing Liao, Quinn Qun Zhu
  • D1-C-terminal processing protease: methods for three dimensional structural determination and rational inhibitor design
    Publication number: 20030175800
    Abstract: The present invention provides atomic coordinate/x-ray diffraction data defining the three dimensional structure of D1 protease. The present invention further provides methods for identifying ligands that bind to D1 protease.
    Type: Application
    Filed: November 15, 2001
    Publication date: September 18, 2003
    Inventors: Bruce A. Diner, Doug B. Jordan, Der-Ing Liao, Mark J. Nelson