Abstract: A method of characterizing specific microbiota associated with Demodex mites from a rosacea patient is described. The method can include a step of realizing a biopsy on the patient and analyzing the microbiota based on a method of 16SrRNA sequencing or pyrosequencing. A method for the diagnosis of rosacea in a patient is also described. This method can include characterizing microbiota associated with Demodex mites in the patient. Also described, are methods of differentiating a subtype of rosacea in a patient with rosacea and methods of controlling and measuring an active agent's treatment effectiveness on rosacea.

Abstract: The present invention concerns the use of a strain of a mycobacterium of the Mycobacterium tuberculosis complex in which a mspA gene capable of expressing a porin A of Mycobacterium smegmatis has been inserted, to produce a vaccine for the prevention of infection with a bacterium of the Mycobacterium tuberculosis complex in a host having eukaryote cells, preferably macrophages, the said strain of mycobacteria thus transformed having reduced growth in the said eukaryote cells.

Abstract: The present invention relates to the use of a compound chosen from squalamine and a squalamine-analogue aminosteroid compound as agent for disinfecting an inert material object, in particular for the pre-disinfection of medical, dental, diagnostic or surgical equipment. The present invention also provides an aqueous or water-soluble disinfecting composition beneficial for a use according to the invention, characterized in that as disinfecting active compound it comprises a said antibacterial and antifungal compound selected from squalamine and a said squalamine-analogue aminosteroid compound and suitable excipients for a water-soluble or aqueous formulation.

Abstract: The present invention relates to a novel mycobacteria culture medium, particularly for mycobacteria of the Mycobacterium tuberculosis complex, that significantly reduces the culture isolation time and thus the time for diagnosing the mycobacteria, particularly those for tuberculosis. A culture medium according to the invention contains defibrinated blood, lecithin, and decomplemented fetal calf serum. The present invention also relates to a culture method and to a method for identifying mycobacteria, particularly bacteria of the Mycobacterium tuberculosis complex. The present invention also relates to a novel decontamination method through chlorhexidine treatment of biological samples in an isolation and mycobacteria culture medium, and to a method for determining by phenotype the sensitivity of mycobacteria to antibiotics by means of a solid culture medium according to the invention.

Abstract: RpoB gene sequences of various species of Acinetobacter bacteria, and a method of detection by molecular identification of various species of Acinetobacter bacteria using rpoB gene sequences.

Abstract: The invention relates to a method for in vitro seriological diagnosis of Whipple's disease, whereby the bacteria responsible for the disease are isolated and established in a culture and brought into contact with the serum or biological fluid of an infected patient. The invention also relates to useful oligonucleotides with a probe and a primer for amplifying, sequencing and detecting the gene rpoB of the bacteria, Tropheryma whippelii.

Abstract: The present invention concerns a method of detection and preferably of quantification of DNA, preferably comprising prokaryotic DNA, extracted from a stool sample of an individual, especially for the molecular determination of the composition of the intestinal flora in the stool. According to the invention, one controls the quality of the DNA extraction by verifying whether one detects a specific DNA of Methanobrevibacter smithii and one performs the quantification of said specific prokaryotic DNA for Archae Methanobrevibacter smithii, for the bacterial genus Lactobacillus, for the phylum Bacteroidetes and for the phylum Firmicutes, respectively, to provide a diagnosis and/or monitoring of the weight status of an individual. The present invention provides a method for carrying out the extraction of prokaryotic DNA in stools.

Abstract: The present invention relates to a novel mycobacteria culture medium, particularly for mycobacteria of the Mycobacterium tuberculosis complex, that significantly reduces the culture isolation time and thus the time for diagnosing the mycobacteria, particularly those for tuberculosis. A culture medium according to the invention contains defibrinated blood, lecithin, and decomplemented fetal calf serum. The present invention also relates to a culture method and to a method for identifying mycobacteria, particularly bacteria of the Mycobacterium tuberculosis complex. The present invention also relates to a novel decontamination method through chlorhexidine treatment of biological samples in an isolation and mycobacteria culture medium, and to a method for determining by phenotype the sensitivity of mycobacteria to antibiotics by means of a solid culture medium according to the invention.

Abstract: The present invention relates to the disclosed transgenic peptides for use in the diagnosis of an infection by intracellular Chlamydia-like bacteria. The invention also relates to a serological diagnostic test. The transgenic peptides are selected from the proteome of Parachlamydia acanthamoebae properties of binding to antibodies of infected human and animals. The test may give further insight in the role of this microorganism in pulmonary diseases and possibly in miscarriage.

Abstract: A method for determining sensitivity or resistance of isolates of HIV (human immunodeficiency virus) retroviruses to chemical molecules having an inhibiting activity on a viral protease or to therapeutic treatments based on inhibitors of the viral protease, including causing cell lysis of at least one yeast by expression of the retrovirus protease.

Abstract: The invention relates to a method of detecting, by means of molecular identification, a bacterium from one of the Staphylococcus-type species. The inventive method is characterised in that the following are used: a fragment of the rpoB gene of said bacterium, comprising a nucleotide sequence selected from one of the SEQ. ID. No. 11 to 39 sequences, the reverse sequences and the complementary sequences; or an oligonucleotide comprising a sequence having at least 12 consecutive nucleotide patterns included in one of the SEQ. ID. No. 7 to 10 sequences, in which N represents a nucleotide selected from inosine and an equimolar mixture of 4 different nucleotides selected from A, T, C or G and from the oligonucleotides of the reverse sequences and complementary sequences.

Abstract: A method for the serological diagnosis, in vitro, of an infection with a spirochete bacterium which is pathogenic in humans, chosen from bacteria of the Borrelia, Leptospira and Treponema genera, by carrying out at least one of 1) an assay of IgG-type and/or IgM-type antibodies against at least one tick-borne Borrelia bacterium, chosen from Borrelia duttonii and Borrelia crocidurae, and 2) an assay of IgM-type antibodies against the louse-borne Borrelia bacterium Borrelia recurrentis.

Abstract: The present invention relates to a method for in vitro diagnosis and follow-up of vaginal bacterial flora status in relation to the presence of bacterial vaginosis, and for monitoring its treatment where applicable, wherein the presence of bacterial vaginosis or failure of on-going treatment is determined if the concentrations of specific sequences present in a single copy in the DNA of the bacteria Atopobium vaginae and Gardnerella vaginalis in the DNA extracted from a vaginal discharge specimen from a patient are such that at least one of the following two conditions a) and b) are met: a) the concentration Ca of said DNA fragment of Atopobium vaginae is greater than or equal to 108 copies/mL, and b) the concentration Cg of said DNA fragment of Gardnerella vaginalis is greater than or equal to 109 copies/mL.

Abstract: A method of determining sensitivity or resistance of isolates of HIV retroviruses to a molecule includes a) amplifying sequences coding for a protease of a retrovirus to be studied, with or without the or some of amino acid sequences situated upstream and downstream of a cleavage site of a precursor in which the amino acid sequences are situated, b) recombining fragments of DNA, a final product of the amplification, and an expression vector allowing expression of sequence coding for the protease of the retrovirus to be studied under control of a known inducible promoter through co-transformation of the vector and the DNA fragments with at least one yeast cell, c) culturing co-transformed yeast cell or cells to obtain a sufficient number of transformants to perform a sensitivity or resistance test, and recovering transformants issuing from the co-transformed cell, on any suitable medium, d) incubating the transformants in the presence of a molecule to be tested, e) qualitatively or quantitatively analyzing the

Abstract: The invention concerns a method for detecting by molecular identification a bacterium of one of the species of genus Streptococcus and four related genera Enterococcus, Gemella, Abiotrophia and Granulicatella which consists of using as probe or primer: the rpoB gene or gene fragment of one said bacterium of sequences SEQ ID no 1 to 3, or an oligonucleotide or mixture of oligonucleotides derived from sequences SEQ ID no 8 to 35, or in particular the oligonucleotides of sequences SEQ ID no 6 and 7.

Abstract: The invention relates to a method for in vitro seriological diagnosis of Whipple's disease, whereby the bacteria responsible for the disease are isolated and established in a culture and brought into contact with the serum or biological fluid of an infected patient. The invention also relates to useful oligonucleotides with a probe and a primer for amplifying, sequencing and detecting the gene rpoB of the bacteria, Tropheryma whippelii.

Abstract: The present invention relates to complete rpoB genes, rpoB gene fragments SEQ. ID. no 9 to 32 and fragments of hypervariable sequences of said rpoB genes SEQ. ID. no 33 to 56 and 77 to 100, and to nucleic acid fragments of non-coding hypervariable sequences flanking the rpoB gene of sequences SEQ. ID. no 121 to 144 and 165 to 188, and also to oligonucleotides of species-specific sequences taken from said fragments of hypervariable sequences and to consensus oligonucleotides between the different species of Acinetobacter bacteria of sequences SEQ. ID. no 1 to 8 for the following 24 species: A. calcoaceticus (genomic species 1), A. baumannii (genomic species 2), genomic species 3, A. haemolyticus (genomic species 4), A. junii (genomic species 5), genomic species 6, A. johnsonii (genomic species 7), A. lwoffii (genomic species 8), genomic species 9, genomic species 10, genomic species 11, A. radioresistens (genomic species 12), genomic species 13, genomic species 16, A. schindleri, A. ursingii, A. baylyi, A.

Abstract: A method for determining sensitivity or resistance of isolates of HIV (human immunodeficiency virus) retroviruses to chemical molecules having an inhibiting activity on a viral protease or to therapeutic treatments based on inhibitors of the viral protease, including causing cell lysis of at least one yeast by expression of the retrovirus protease.

Abstract: The invention relates to a method for in vitro seriological diagnosis of Whipple's disease, whereby the bacteria responsible for the disease are isolated and established in a culture and brought into contact with the serum or biological fluid of an infected patient. The invention also relates to useful oligonucelotides with a probe and a primer for amplifying, sequencing and detecting the gene rpoB of the bacteria, Tropheryma whippelii.

Abstract: The invention concerns the total sequence of the Tropheryma whippelii bacteria rpoB gene, characterized in that it consists of the nucleotide sequence SEQ ID no. °9, and the nucleotide sequences derived from said sequence specific of the Tropheryma whippelii bacteria and their use as probe and primer.