Abstract: The invention concerns single stranded oligonucleotides comprising a sequence of at least 12 consecutive nucleotide motifs contained in one of the sequences SEQ ID NO: 1 to SEQ ID NO: 4 wherein “n” represents an identical or different nucleotide selected among inosine or an equimolar mixture of 4 different nucleotides selected among a, t, c or g, and among oligonucleotides complementary thereto. The invention also concerns using the oligonucleotides to detect bacteria of the Spirochaetales order. The invention is based on the use of specifically defined sequences in the rpoB gene of spirochetes coding for the beta subunit of the bacterial RNA polymerase.

Abstract: The invention concerns a method for detecting by molecular identification a bacterium of one of the species of genus Streptococcus and four related genera Enterococcus, Gemella, Abiotrophia and Granulicatella which consists of using as probe or primer: the rpoB gene or gene fragment of one said bacterium of sequences SEQ ID no 1 to 3, or an oligonucleotide or mixture of oligonucleotides derived from sequences SEQ ID no 8 to 35, or in particular the oligonucleotides of sequences SEQ ID no 6 and 7.

Abstract: The invention concerns the use of an antigen containing protein A, and in particular of Staphylococcus aureus as non-specific antigen added to bacterial serology reactions as positive control that the serum to be tested has been included.

Abstract: The present invention pertains to an in vitro serological diagnosis method for blood culture-negative infective endocarditis caused by the bacteria Coxellia burnetii and Bartonella sp in a patient suffering from a heart valve disorder, wherein an assay is performed of type IgG antibodies of the bacterium Coxellia burnetii phase 1 and of type IgG antibodies of a Bartonella sp bacterium, on one same serum specimen from the said patient, and it is determined whether the titre of said IgG antibodies is equal to or greater than 1:800.

Abstract: The invention relates to a method of detecting, by means of molecular identification, a bacterium from one of the Staphylococcus-type species. The invention method is characterised in that the following are used: a fragment of the rpoB gene of said bacterium, comprising a nucleotide sequence selected from one of the SEQ ID. no. 11 to 39 sequences, that reverse sequences, the reverse sequences and the complimentary sequences; or an oligonucleotide comprising a sequence having at least 12 consecutive nucleotide patterns included in one of the SEQ.ID. no. 7 to 10 sequences, in which N represents a nucleotide selected from inosine and an eqimolar mixture of 4 different nucleotides selected from A, T, C or G and from the oligonucleotides of the reverse sequences and complementary sequences.

Abstract: The invention concerns the total sequence of the Tropheryma whippelii bacteria rpoB gene, characterized in that it consists of the nucleotide sequence SEQ ID n°9, and the nucleotide sequences derived from said sequence specific of the Tropheryma whippelii bacteria and their use as probe and primer.

Abstract: The invention concerns a method for isolating Rickettsia pulicis bacterium on clawed frog cells or any other cell at a culture temperature of 28°. The bacterium is used for a serological diagnostic method which consists in contacting the bacterium responsible for said disease with the patient's serum or biological fluid. The invention also concerns a device for implementing said method for in vitro detection of the bacterium in fleas or human samples.

Abstract: The invention relates to oligonucleotide primers and probes that can specifically hybridize to the rDNA or rRNA of at least one species of the genus Rickettsia.