Abstract: An improved method is provided for conducting chemical or biochemical reactions on a substrate surface in which the reactions take place in an enclosed chamber between components of a fluid and molecular moieties present on an interior surface of the chamber, wherein the improvement involves mixing the fluid during the reactions and maximizing contact between the fluid's components and the entirety of the reactive interior surface by (a) applying centrifugal force to the chamber and simultaneously (b) rotating the chamber about an axis thereof. The improved method is particularly advantageous in the context of a hybridization assay in which molecular components within a sample fluid hybridize to surface-bound molecular probes (e.g., as may be present in a spatially defined and physically addressable array) within a hybridization chamber, and wherein process and device parameters (e.g., sample volume, chamber volume, temperature, number of parts and materials, and the like) are critical.

Abstract: Mechanically elongated neuronal cells and methods of mechanically producing elongated cells are provided. Also provided are methods for transplanting elongated neuronal cells into an animal for treatment of spinal cord injuries and other nerve injuries.

Abstract: A hub (2) includes a spindle (14) which projects through a housing (4) and rotates relative to the housing (4) on a bearing (6) that is located between the spindle (14) and the housing (4). The bearing (6) has two sets of raceways (28,40) that are oblique to the axis x, and in addition rolling elements (36) arranged in two rows between the sets of raceways (28,40). The inner raceways (28) that fit around the spindle (14) and have back faces (32), with the back face (32) for one of the races (26) being against a shoulder (18) from which the spindle (14) projects. Initially, the end of the spindle (14) projects straight beyond the back face (32) of the outer race—indeed, so that the races (26) can be installed over the spindle (14).

Abstract: Mechanically elongated neuronal cells and methods of mechanically producing elongated cells are provided. Also provided are methods for transplanting elongated neuronal cells into an animal for treatment of spinal cord injuries and other nerve injuries.

Abstract: Novel multispecific chimeric receptor DNA sequences, expression cassettes and vectors containing these sequences as well as cells containing the chimeric DNA and novel chimeric receptor proteins expressed from the sequences are provided in the present invention. The novel multispecific chimeric receptor DNA and amino acid sequences comprise at least three domains that do not naturally exist together: (1) a multispecific binding domain comprising at least two extracellular inducer-responsive clustering domains which serves to bind at least one specific inducer molecule, (2) a transmembrane domain, which crosses the plasma membrane, and (3) either a proliferation signaling domain that signals the cell to divide, or an effector function signaling domain which directs a host cell to perform its specialized function.

Abstract: The present invention is directed to novel chimeric proliferation receptor proteins and DNA sequences encoding these proteins where the chimeric proteins are characterized in three general categories. In one category, the novel chimeric proteins comprise at least three domains, namely, an extracellular inducer-responsive clustering domain capable of binding an extracellular inducer that transmits a signal to a proliferation signaling domain, a transmembrane domain and a proliferation signaling domain that signals a host cell to divide. In the second category, the novel chimeric proteins comprise at least two domains, namely, an intracellular inducer-responsive clustering domain capable of binding an intracellular inducer and a proliferation signaling domain that signals the cell to divide.

Abstract: The invention relates the discovery that the polymorphisms in 16S rRNA genes may be used to identify unknown bacterial isolates at the level of species, subspecies and strain identification. The invention also relates to the discovery that the sequences of individual are required. The invention involves the generation of composite polynucleotide sequence of the 16S rRNA genes of a microorganism of interest. The invention is to provide methods for identifying microorganisms. The methods comprise the steps of generating a composite 16S rRNA region sequence from a microorganism of interest. The composite 16S rRNA region sequence reveals polymorphisms within the 16S ribosomal RNA gene of the microorganism. The composite 16S rRNA region sequence may then be compared with a list of previously obtained composite 16S rRNA region sequences from reference microorganisms so as to determine the species, subspecies, or strain of the microorganism of interest.

Abstract: The present invention relates to improved methods of generating polynucleotide sequencing reaction products from cycle sequencing and relates to various instruments and reagents for use in the subject methods. The use of "step-down" thermal profiles in conjunction with cycle sequencing is described. Step-down thermal profiles are formed by combining several thermal cycle sets such that the annealing temperature of each thermal cycle set is less than the annealing temperature of the preceding thermal cycle set. One embodiment of the invention is a method of generating a plurality of polynucleotide sequencing reaction products in parallel by subjecting a plurality of sequencing solution preparations to a step-down thermal profile, i.e., exposure to repeated thermal cycle sets, each thermal cycle set having an annealing temperature that is lower than the annealing temperature of the annealing phases of the preceding thermal cycle set.

Abstract: An interactive game apparatus in which players throw projectiles, such as soft balls, against a display panel on which a target image is projected and contact sensitive elements aligned with target areas in the projected image are activated by a hit from the thrown projectile to initiate a change in the projected image uniquely associated with the target area hit and the player who threw the projectile. An embodiment in the context of a tic-tac-toe game is described. A number of embodiments for identifying specific projectiles with a player responsible for throwing the projectile are described.

Abstract: Apparatus are disclosed that thermally cycles samples between at least two temperatures. These apparatus operate by impinging fluid jets onto the outer walls of a sample containing region. Because the impinging fluid jets provide a high heat transfer coefficient between the jet and the sample containing region, the sample containing regions are uniformly cycled between the two temperatures. The heat exchange rate between the jets and the sample regions are substantially uniform.

Abstract: A combined bearing and sensor assembly comprises a sensor unit 30 and a bearing unit 40. The sensor unit 30 includes a coupling ring 10 which has a radially inwardly directed lip 22 which engages with a groove 11 formed in the inner raceway 18 of the bearing unit 40. The coupling ring 10 also has an axially extending portion 5, the radially inwardly facing surface of which engages with a sensor unit mounting surface 8a such that the sensor unit's engagement to the mounting surface 8a is greater than its engagement to the bearing unit 40. In this way the combined bearing and sensor unit may be sold, transported and fitted as a single unit, but will separate to allow the sensor unit 30 to remain properly mounted when the bearing unit 40 is dismounted for general maintenance purposes.

Abstract: The present invention is directed to novel chimeric proliferation receptor proteins and DNA sequences encoding these proteins where the chimeric proteins are characterized in three general categories. In one category, the novel chimeric proteins comprise at least three domains, namely, an extracellular inducer-responsive clustering domain capable of binding an extracellular inducer that transmits a signal to a proliferation signaling domain, a transmembrane domain and a proliferation signaling domain that signals a host cell to divide. In the second category, the novel chimeric proteins comprise at least two domains, namely, an intracellular inducer-responsive clustering domain capable of binding an intracellular inducer and a proliferation signaling domain that signals the cell to divide.

Abstract: A novel means for providing an internal damping mechanism for a system with structural load supporting members that may be subject to time-varying loads, where the structure's structural members have rough holes formed in them, either by casting, drilling, or welding pipe, where the holes can be any shape, but the ideal shape and location maximizes the product of the perimeter of the shape and the distance from the neutral axes to the neutral axis of the structure, so a smooth surfaced shear member, with a diameter several millimeters smaller than the hole and a surface finish on the order of 0.5 micron R.sub.

Abstract: The present invention is directed to novel chimeric proliferation receptor proteins and DNA sequences encoding these proteins where the chimeric proteins are characterized in three general categories. In one category, the novel chimeric proteins comprise at least three domains, namely, an extracellular inducer-responsive clustering domain capable of binding an extracellular inducer that transmits a signal to a proliferation signaling domain, a transmembrane domain and a proliferation signaling domain that signals a host cell to divide. In the second category, the novel chimeric proteins comprise at least two domains, namely, an intracellular inducer-responsive clustering domain capable of binding an intracellular inducer and a proliferation signaling domain that signals the cell to divide.

Abstract: A gel electrophoresis separation apparatus comprising, in combination, a gaseous heat-exchange medium, a gaseous heat-exchange medium driving means, and an impingement means, whereby the gaseous heat-exchange medium is driven by the gaseous heat-exchange medium driving means across the impingement means to provide a flow of the gaseous heat-exchange medium on the surface of the gel plates, whereby the flow induced by passage of the gaseous heat-exchange medium through the impingement means thereby minimizes temperature gradients within the gel by forced convection.

Abstract: A vial transport apparatus which is capable of storing and retrieving samples and then transporting them from one location in a sampling system to another without the complexity and expense of robotic arms or gripper elements is provided. A passive hook arrangement is utilized to engage the vials for transport and can be operated using a standard three motor system for transport in the X, Y, and Z directions. The hook supports the vial from beneath and at least partially surrounds the vial to entrap it during transport thereof.

Abstract: A rotary scanning apparatus for scanning a plurality of separation lanes where the separation lanes are disposed in a nonplanar array. The scanning apparatus includes an optical detection system for detecting radiation emanating from the separation lanes, the optical detection system including collection optics for collecting and focusing the radiation, a detector for measuring the intensity of the radiation, and a rotary scanner for providing relative movement between the collection optics and the separation lanes.

Abstract: A dual image station photoprint scanner comprises a cabinet having an upper, album imaging station and a lower, magazine-fed, platen imaging station. The upper imaging station includes a translatable unit having a glass plate upon which a photoprint is placed in a face- down position. Adjacent to the top plate, the translatable cabinet has a sloped support surface, to accommodate a photo album. A lower portion of the cabinet contains a platen feed mechanism, which translates a photographic print support platen from a supply magazine to a platen imaging station, and then feeds the platen to a storage magazine. A high resolution CCD image sensor is supported at an image projection plane of a multidirectional image projection mechanism. A digital imagery data recording mechanism is coupled to the image sensor and is operative to record, on a digital data storage medium, a digitized image that has been projected on the image sensor.

Abstract: A gas chromatograph system includes a central processor (CPU) and a printer-plotter. The printer-plotter prints characters and plots data using a single 1 by 7 array of thermal resistors to perform both the printing and plotting operations in real time. The system provides continuous and uniform traces despite changes in the plotting rate.Data from the chromatograph is temporarily stored by the CPU, then forwarded upon programmed command through a print-plot controller to the printer-plotter. Data is plotted as it is received from the CPU. As peaks in the data are detected, plotting is suspended temporarily without loss of data, and characters are printed adjacent to the peaks, thus annotating the peaks with digital data.