Abstract: A method of treatment of a patient suffering from autonomic dysfunction is described. The method of treatment can include the steps of initially evaluating the patient and administering buprenorphine to treat the patient, for example, daily. The patient undergoing such treatment is subjected to monitoring. The monitoring of the patient may be on a periodic basis, for example, a weekly basis, a daily basis, or on a real-time basis. Such monitoring may be through any one or combination of a plurality of bodily measurements that includes, for example, heart rate variability or blood catecholamine concentrations. Monitoring of the patient may also occur remotely. Methods are also described for determining whether the amount of buprenorphine provided to the patient may be tapered.

Abstract: A method of treatment of a subject suffering from autonomic dysfunction due to opioid use is described. The autonomic dysfunction can be determined by, for example, detecting genetic damage, such as DNA hypermethylation, measuring catecholamine concentrations, collecting vitals data, and/or surveys. The method of treatment can include administering a partial opioid agonist, such as buprenorphine.

Abstract: Methods for producing a paired tag from a nucleic acid sequence are provided in which the paired tag comprises the 5? end tag and 3? end tag of the nucleic acid sequence. In one embodiment, the nucleic acid sequence comprises two restriction endonuclease recognition sites specific for a restriction endonuclease that cleaves the nucleic acid sequence distally to the restriction endonuclease recognition sites. In another embodiment, the nucleic acid sequence further comprises restriction endonuclease recognition sites specific for a rare cutting restriction endonuclease. Methods of using paired tags are also provided. In one embodiment, paired tags are used to characterize a nucleic acid sequence. In a particular embodiment, the nucleic acid sequence is a genome. In one embodiment, the characterization of a nucleic acid sequence is karyotyping. Alternatively, in another embodiment, the characterization of a nucleic acid sequence is mapping of the sequence.

Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

Abstract: The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.

Abstract: The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.

Abstract: A three-way hitch assembly for a tractor-type vehicle, such as an ATV/UTV, is designed to facilitate the attachment of multiple accessory devices to the vehicle. The hitch assembly includes: a drawbar pintle, a hook, and a replaceable hitch ball.

Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

Abstract: A pair of asymmetrical, partially double-stranded oligonucleotide adapters are provided wherein the pair of adapters comprise a first asymmetrical oligonucleotide adapter comprising a single-stranded 3? overhang and a second asymmetrical double-stranded oligonucleotide adapter comprising a single-stranded 5? overhang and at least one blocking group on the strand of said second asymmetrical oligonucleotide adapter that does not comprise the 5? overhang. Also provided are a pair of double-stranded Y oligonucleotide adapters and a pair of double-stranded bubble oligonucleotide adapters and methods of using said asymmetrical adapters for amplification of at least one double stranded nucleic acid molecule, wherein the amplification produces a plurality of amplified nucleic acid molecules having a different nucleic acid sequence at each end are also described. Also provided is a method for exponentially amplifying one strand in a double-stranded nucleic acid molecule.

Abstract: A method for fabricating a nozzle includes providing at least two plates. At least one of the at least two plates includes a first opening. Each plate includes at least one mating surface. The method also includes stacking the at least two plates together such that a mating surface of a first of the at least two plates is substantially flush against a mating surface of a second of the at least two plates, and such that a fluid passage is at least partially defined by the at least two plates. The method also includes orienting the at least two plates relative to each other such that the opening within at least one of the at least two plates at least partially defines an outlet for discharging fluid from the fluid passage.

Abstract: Methods for producing a paired tag from a nucleic acid sequence are provided in which the paired tag comprises the 5? end tag and 3? end tag of the nucleic acid sequence. In one embodiment, the nucleic acid sequence comprises two restriction endonuclease recognition sites specific for a restriction endonuclease that cleaves the nucleic acid sequence distally to the restriction endonuclease recognition sites. In another embodiment, the nucleic acid sequence further comprises restriction endonuclease recognition sites specific for a rare cutting restriction endonuclease. Methods of using paired tags are also provided. In one embodiment, paired tags are used to characterize a nucleic acid sequence. In a particular embodiment, the nucleic acid sequence is a genome. In one embodiment, the characterization of a nucleic acid sequence is karyotyping. Alternatively, in another embodiment, the characterization of a nucleic acid sequence is mapping of the sequence.

Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

Abstract: The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.

Abstract: A manually operated lifting device for adaptably lifting the front or back end of a lawn mower, garden tractor or similar small, powered vehicle, or to lift these vehicles from the side, is provided. The lifting device comprises a vertical frame having removable front arms at the bottom end and extending forward for engaging the axle of the mower or tractor, either at the front or back. The lifting device also comprises a horizontal arm at the bottom of the frame, for positioning and securing the vehicle while being lifted from the side, a collapsible base member and a threaded rod connect to the base member and the frame, such that turning the rod raises the frame from the base, thus lifting the vehicle. The rod may be turned using a hand crank or an electrical device such as an electric hand drill.

Abstract: A three-way hitch assembly for a tractor-type vehicle, such as an ATV/UTV, is designed facilitates the attachment of multiple accessory devices to the vehicle. The hitch assembly includes: (a) a receiver portion adapted to be attached to a vehicle, said receiver portion having a profiled opening for receiving a drawbar portion; (b) a drawbar portion having a matching profile to said receiver portion and adapted to be inserted in the receiver portion in one of a plurality of orientations as required for a particular application; (c) means for retaining the drawbar portion in the receiver portion in said one orientation; (d) a hook attached to one side of the drawbar portion adapted to receive a tow chain or strap; (e) a pintle attached to one side of the drawbar portion adapted to receive a pin/pintle attachment accessory; and (f) a trailer ball attached to one side of the drawbar portion adapted to receive a trailer hitch mount.

Abstract: The present teachings relate to improved methods, kits, and compositions for making nucleic acid libraries and sequencing nucleic acids. In some embodiments, directionally defined concatamers are generated, facilitating sequencing efforts.

Abstract: A weighted training bat formed of wood and having a handle and a barrel. The barrel has an elongated hollow cavity formed into a distal end thereof and an outer cavity portion having internal threads. Lead which has been poured or cast-in-place into and filling the proximal portion of the cavity, when solidified, adheres to the interior surface of the cavity to resist loosening, the lead adding weight at and evenly astride the sweet spot of the bat. An elongated wooden screw having external threads is tightly engaged into internal threads in the cavity so the screw bears against the lead to further prevent loosening of the lead. The threads are permanently glued and an outer end of the screw is formed to blend with the contour of the end of the barrel.

Abstract: A system for machining a component with complex contour. The machining system includes a coolant flow nozzle with an interior passage with a first portion having a first cross-sectional shape, and a second portion having a second cross-sectional shape, with the second cross-sectional shape selected so that fluid discharged from the nozzle has a selected cross-sectional shape. A method of fabricating a coolant flow nozzle with an interior passage that has a varying cross sectional shape.