Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.

Abstract: Proto-antigen-presenting surfaces and related kits, methods, and uses are provided. The proto-antigen-presenting surface can comprise a plurality of primary activating molecular ligands comprising a major histocompatibility complex (MHC) molecule configured to bind to a T cell receptor (TCR) of a T cell and a plurality of co-activating molecular ligands each including a TCR co-activating molecule or an adjunct TCR activating molecule, wherein an exchange factor is bound to the MHC molecules. Exchange factors include, e.g., dipeptides such as GL, GF, GR, etc. Proto-antigen-presenting surfaces can be used to rapidly prepare antigen-presenting surfaces comprising one or more peptide antigens of interest by contacting the proto-antigen-presenting surface with one or more peptide antigens so as to displace the exchange factor. As such, the disclosure facilitates rapid evaluation of the immunogenicity of peptide antigens for activating T lymphocytes.

Abstract: A computer-implemented method for providing account holders with a reward based on similar rewards previously obtained through other accounts. The method includes identifying, using one or more computer processors configured to access an account database, an eligible account based on eligibility criteria, wherein the eligible account is associated with an account of an account holder stored in the account database; receiving identifying data that identifies the eligible account; associating a customer identifier with the identifying data from the eligible account; for each customer identifier, receiving confirmation data associated with a separate account of the account holder, wherein the confirmation data comprises a reward variable; validating, using one or more computer processors, the confirmation data; and rewarding the eligible account with a matching variable, wherein the matching variable is approximately equal to the reward variable.

Abstract: A computer-implemented method for providing account holders with a reward based on similar rewards previously obtained through other accounts. The method includes identifying, using one or more computer processors configured to access an account database, an eligible account based on eligibility criteria, wherein the eligible account is associated with an account of an account holder stored in the account database; receiving identifying data that identifies the eligible account; associating a customer identifier with the identifying data from the eligible account; for each customer identifier, receiving confirmation data associated with a separate account of the account holder, wherein the confirmation data comprises a reward variable; validating, using one or more computer processors, the confirmation data; and rewarding the eligible account with a matching variable, wherein the matching variable is approximately equal to the reward variable.

Abstract: Textured fibrous structures, and more particularly textured fibrous structures having a plurality of deformations such that the textures fibrous structures exhibit novel surface height properties compared to known fibrous structures, and methods for making such textured fibrous structures.

Abstract: Disclosed herein, a power supply may be recharged with a generally used system. In various embodiments, cleaning systems may be used to affect a generator to cause the generator to charge a power supply. Such charging systems may be used without the need for separate or specialized power charging systems. A charging source or system may, therefore, also charge a power supply without requiring additional steps.

Abstract: The disclosure provides methods for amplification of paired transcript sequences from a single cell, such as, but not limited to alpha and beta T cell receptor (TCR) sequences from a single T cell. The method can include: placing a single T cell into a cell lysis solution to provide a T cell lysate comprising RNA; generating first strand cDNA from the RNA; amplifying the first strand cDNA to provide amplified cDNA; and amplifying the alpha and beta TCR sequences from the amplified cDNA in a single reaction, wherein the alpha and beta TCR sequences are amplified using three sets of TCR amplification primers. In some instances, the methods can be performed using no more than six sets of primers.

Abstract: A combustor dome may be formed by way of additive layer manufacturing. The combustor dome may further include a raised outer surface and a recessed outer surface on a hot side of the combustor dome. The recessed outer surfaces may be closer to the cold side than the raised outer surfaces. The combustor dome may include a shadow surface defined between the raised outer surface and recessed outer surface. The shadow surface may define a corresponding cooling outlet in fluid communication with an internal cooling channel defined inside of the combustor dome. The cooling outlet may release air from the internal cooling channel to the hot axial side of the combustor dome.

Abstract: A dowel basket assembly apparatus includes a jig table rotationally supported on a bearing assembly, which supports a table top assembly having arms extending radially outwardly and supporting first, second and third jig table bases. The bases move through first, second and third stations by rotating the assembly. First jig table is positioned at first station, second jig table at second station, and third jig table at third station. Rods are located in receptacles on first jig table base at first station. Simultaneously, rods are welded to form a dowel basket on second jig table base at second station. Simultaneously, a welded dowel basket is unloaded from third jig table base at third station. After completion of the steps of locating, welding and retrieving, the apparatus is rotated to advance the jig tables to the next station.

Abstract: Textured fibrous structures, and more particularly textured fibrous structures having a plurality of deformations such that the textures fibrous structures exhibit novel surface height properties compared to known fibrous structures, and methods for making such textured fibrous structures.

Abstract: Textured fibrous structures, and more particularly textured fibrous structures having a plurality of deformations such that the textures fibrous structures exhibit novel surface height properties compared to known fibrous structures, and methods for making such textured fibrous structures.

Abstract: Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.

Abstract: A combustor liner assembly is provided including an inlet wall, an inner wall, an outer wall, and a plurality of fasteners. The inlet wall has an opening into a combustion chamber. The inner wall is coupled to the inlet wall at a first end of the combustor liner. The inner wall defines a radially inner end of the combustion chamber. The outer wall includes a plurality of segments. The plurality of segments define a radially outer end of the combustion chamber. A first portion of the plurality of fasteners couples each of the plurality of segments to another of the plurality of segments. A second portion of the plurality of fasteners couples at least one of the plurality of segments to the inlet wall at the first end of the combustor liner.

Abstract: Processes and kits are provided for producing sequence specific fragments of nucleic acid molecules, whether from a genome or transcriptome, where one end of the molecule is highly diverse and/or the full-length molecule, whether a gene or a mRNA, is too long for it to be sequenced using currently available sequencing methods. Methods of preparing a sequencing library configured for 5? or 3? anchored sequencing, wherein the opposing termini of the library molecules are differentially truncated, and methods of parallel sequencing such libraries are described.

Abstract: This disclosure relates to the production and use of an isolated, purified and/or recombinant T cell receptor (TCR) that specifically binds to a mutant IDH1 protein, or a fragment thereof, wherein the mutant IDH1 protein or fragment thereof comprises an R132H mutation.

Abstract: Disclosed herein, a power supply may be recharged with a generally used system. In various embodiments, cleaning systems may be used to affect a generator to cause the generator to charge a power supply. Such charging systems may be used without the need for separate or specialized power charging systems. A charging source or system may, therefore, also charge a power supply without requiring additional steps.

Abstract: Textured fibrous structures, and more particularly textured fibrous structures having a plurality of deformations such that the textures fibrous structures exhibit novel surface height properties compared to known fibrous structures, and methods for making such textured fibrous structures.

Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to activate T Lymphocytes.

Abstract: A computer-implemented method for providing account holders with a reward based on similar rewards previously obtained through other accounts. The method includes identifying, using one or more computer processors configured to access an account database, an eligible account based on eligibility criteria, wherein the eligible account is associated with an account of an account holder stored in the account database; receiving identifying data that identifies the eligible account; associating a customer identifier with the identifying data from the eligible account; for each customer identifier, receiving confirmation data associated with a separate account of the account holder, wherein the confirmation data comprises a reward variable; validating, using one or more computer processors, the confirmation data; and rewarding the eligible account with a matching variable, wherein the matching variable is approximately equal to the reward variable.

Abstract: Disclosed herein, a power supply may be recharged with a generally used system. In various embodiments, cleaning systems may be used to affect a generator to cause the generator to charge a power supply. Such charging systems may be used without the need for separate or specialized power charging systems. A charging source or system may, therefore, also charge a power supply without requiring additional steps.