Abstract: Compositions and methods for treating or preventing a fungal plant disease are provided. Such compositions and methods comprise a combination of one or more chemical fungicides and one or more fungicidal bacterial or fungal strains, or an active variant thereof, that controls one or more fungal pathogens that cause fungal plant disease. Methods for growing a plant susceptible to a fungal plant disease and methods for controlling a fungal plant pathogen are also provided.

Abstract: The instant disclosure describes the application of genetic engineering techniques to produce cellulase in plants. Cellulase coding sequences operably linked to promoters active in plants may be transformed into the nuclear genome and/or the plastid genome of a plant. As cellulases may be toxic to plants, chemically-inducible or wound-inducible promoters may be employed. Additionally, the expressed cellulases may be targeted to vacuoles or other cellular organelles.

Abstract: The instant disclosure describes the application of genetic engineering techniques to produce cellulase in plants. Cellulase coding sequences operably linked to promoters active in plants may be transformed into the nuclear genome and/or the plastid genome of a plant. As cellulases may be toxic to plants, chemically-inducible or wound-inducible promoters may be employed. Additionally, the expressed cellulases may be targeted to vacuoles or other cellular organelles.

Abstract: The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an herbicide tolerant form of a plant protox enzyme. The present invention further provides shuffled DNA molecules encoding protox enzymes having enhanced tolerance to a herbicide that inhibits the protox activity encoded by a template DNA molecule from which the shuffled DNA molecule is derived.

Abstract: Disclosed are novel DNA sequences isolated from soybean, wheat, cotton, sugar beet, rape, rice, sorghum, and sugar cane encode enzymes having protoporphyrinogen oxidase (protox) activity. In addition, modified, herbicide-tolerant forms of protox enzymes are disclosed. Plants expressing herbicide-tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an inhibitor-resistant from of a plant protox enzyme. Further disclosed is a method of achieving herbicide tolerance through plastid transformation, which involves transforming plastids with a chimeric gene that comprises a plastid-active promoter operatively linked to a DNA molecule that encodes a plastid-targeted enzyme whose native plastid transit peptide is either mutated so as to be nonfunctional or is absent altogether.

Abstract: The invention provides novel methods of controlling gene expression in plastids, using an inducible, transactivator-mcdiated system, and plants comprising the novel expression systems. The present invention further describes the production of cellulose-degrading enzymes in plants via the application of genetic engineering techniques. Cellulase coding sequences are fused to promoters active in plants and transformed into the nuclear genome and the chloroplast genome. As cellulases may be toxic to plants, preferred promoters are those that are chemically-inducible. In this manner, expression of the cellulase genes transformed into plants may be chemically induced at an appropriate time. In addition, the expressed cellulases may be targeted to vacuoles or other organelles to alleviate toxicity problems. The present invention finds utility in any industrial process requiring a plentiful supply of cellulases, but particularly finds utility in the conversion of cellulosic biomass to ethanol.

Abstract: The present invention relates to methods for controlling the growth of undesired vegetation comprising applying an effective amount of a protox-inhibiting herbicide to a population of transgenic plants or plant seed transformed with a DNA sequence coding for a modified protox enzyme that is tolerant to a protox-inhibiting herbicide or to the locus where a population of the transgenic plants or plant seeds is cultivated.

Abstract: The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides are also provided. These plants are engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they are transformed with modified eukaryotic or prokaryotic protox coding sequences or wild type prokaryotic protox sequences which are herbicide tolerant. Plant genes encoding wild-type and altered protox, purified plant protox, methods of isolating protox from plants, and methods of using protox-encoding genes are also disclosed.

Abstract: The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides and a method of selecting transformed plants are also provided.

Abstract: The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. A method for controlling weeds using plants having altered protox activity which confers tolerance to herbicides is described.

Abstract: The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant . Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an herbicide tolerant form of a plant protox enzyme. The present invention further provides shuffled DNA molecules encoding protox enzymes having enhanced tolerance to a herbicide that inhibits the protox activity encoded by a template DNA molecule from which the shuffled DNA molecule is derived.

Abstract: The present invention provides novel eukaryotic DNA sequences coding for native protoporphyrinogen oxidase (protox) or modified forms of the enzyme which are herbicide tolerant. Plants having altered protox activity which confers tolerance to herbicides are also provided. These plants may be bred or engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or through increased levels of expression of the native protox gene, or they may be transformed with modified eukaryotic or prokaryotic protox coding sequences or wild type prokaryotic protox sequences which are herbicide tolerant. Diagnostic and other uses for the novel eukaryotic protox sequence are also described. Plant genes encoding wild-type and altered protox, purified plant protox, methods of isolating protox from plants, and methods of using protox-encoding genes are also disclosed.

Abstract: The present invention provides novel DNA sequences coding for protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, oilseed rape, rice, sorghum, and sugar cane. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an herbicide tolerant form of a plant protox enzyme.

Abstract: The present invention provides novel DNA sequences isolated from soybean, wheat, cotton, sugar beet, rape, rice, sorghum, and sugar cane that coding for enzymes having protoporphyrinogen oxidase (protox) activity. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an inhibitor-resistant from of a plant protox enzyme.

Abstract: Promoters naturally associated with plant protoporphyrinogen oxidase (protox) coding sequences, and derivatives thereof, are provided. These promoters can be used to control the expression of an operably linked heterologous coding sequence in a plant cell. These promoters are particularly useful for expressing modified forms of herbicide target enzymes, particularly modified forms of protox, to achieve tolerance to herbicides that inhibit the corresponding unmodified enzymes. Recombinant DNA molecules and chimeric genes comprising these promoters are provided, as well as plant tissue and plants containing such chimeric genes.

Abstract: The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease.

Abstract: The present invention provides novel DNA sequences coding for plant protoporphyrinogen oxidase (protox) enzymes from soybean, wheat, cotton, sugar beet, rape, rice and sorghum. In addition, the present invention teaches modified forms of protox enzymes that are herbicide tolerant. Plants expressing herbicide tolerant protox enzymes taught herein are also provided. These plants may be engineered for resistance to protox inhibitors via mutation of the native protox gene to a resistant form or they may be transformed with a gene encoding an inhibitor-resistant from of a plant protox enzyme.

Abstract: The present invention provides novel plant DNA sequences coding for native adenylosuccinate synthetase (ADSS). Methods for using the complete or partial ADSS coding sequence as a probe for diagnostic, mapping and other purposes are taught. Generation of transformed host cells capable of expressing ADSS is also taught. Methods of using the transformed host cells are taught, including methods for recombinant production of ADSS enzymes. A method for using the plant ADSS enzyme to screen for inhibitors of ADSS activity is also provided.

Abstract: The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease.

Abstract: The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease.