Abstract: Methods and compositions are provided for amplifying RNA from an RNA template. Methods and compositions are further provided for detecting an RNA in an RNA containing sample. Compositions used for the amplification of RNA from RNA include a RNA-dependent RNA-polymerase, a RNA helicase, and an energy source. Illustrative RNA-dependent RNA-polymerase enzymes are derived from the tomato or tobacco plant, while illustrative RNA helicase enzymes include the eIF4A and eIF4B proteins.

Abstract: Methods and compositions are provided for enhanced specificity and sensitivity of amplification reaction mixtures. Compositions in accordance with the present invention provide for reduced formation of primer-aggregates during amplification reactions. Reaction mixes include dNTPs, where a portion of the dNTPs has been replaced with an unconventional nucleotide, e.g., dUTP. Unconventional nucleotide concentrations are typically between 10% to 50% of the concentration of one of the standard dNTP. In some compositions the unconventional nucleotide is dUTP which replaces from about 10% to about 50% of the dTTP in the dNTP mix.

Abstract: The invention provides Pol III ? mutants, and modified Pol III replicases comprising the same, and methods of using modified Pol III replicases for a variety of nucleic acid replication reactions.

Abstract: The invention provides one-component and two-component DNA polymerases, as well as kits comprising the same, and methods of using the same for nucleic amplification and nucleic acid sequencing.

Abstract: Methods and compositions are provided for amplifying RNA from an RNA template. Methods and compositions are further provided for detecting an RNA in an RNA containing sample. Compositions used for the amplification of RNA from RNA include a RNA-dependent RNA-polymerase, a RNA helicase, and an energy source. Illustrative RNA-dependent RNA-polymerase enzymes are derived from the tomato or tobacco plant, while illustrative RNA helicase enzymes include the eIF4A and eIF4B proteins.

Abstract: The present invention relates to a detection and quantification method of different sets of target molecules (1, 3), being possibly present simultaneously in a biological sample by the use of a microarray present upon a solid support surface (6) and comprising different sets (A, B) of capture molecules (2, 4), present in different locations (8, 9) of the solid support surface (6).

Abstract: Compositions and methods are provided for the in vitro amplification of nucleic acid and, in particular, for the amplification of extrachromosomal nucleic acid molecules having a molecular weight of one kilobase or greater. The design and use of primers, low ionic strength amplification buffer, low co-solvent containing buffer, and combined polymerization/ligation reaction buffers are described in this regard.

Abstract: In some embodiments, a method, apparatus and system for enabling and disabling voltage regulator controllers are generally presented. In this regard, a sequencer agent is introduced to selectively enable or disable the outputs of voltage regulator controllers in an electronic appliance based at least in part on settings stored in non-volatile memory. Other embodiments are discussed and claimed.

Abstract: As shown in FIG.

Abstract: According to one embodiment, an apparatus is disclosed. The apparatus includes a printed circuit board (PCB), a connector mounted on the PCB, and an integrated circuit (IC) package for insertion into the connector. The IC package includes a plurality of lands having a varied pitch distance.

Abstract: The present invention provides a method of making a fluoropolymer comprising repeating units derived from one or more gaseous fluorinated monomers. The method comprises an aqueous emulsion polymerization of gaseous fluorinated monomers in the presence of an ether selected from the group consisting of dimethyl ether (DME), methyl tertiary butyl ether (MTBE) and mixtures thereof.

Abstract: The present invention provides a method of making a fluoropolymer comprising repeating units derived from one or more gaseous fluorinated monomers. The method comprises an aqueous emulsion polymerization of gaseous fluorinated monomers in the presence of an ether selected from the group consisting of dimethyl ether (DME), methyl tertiary butyl ether (MTBE) and mixtures thereof.

Abstract: A split cylindrical clamp encloses inner ends of grip bars of a folding handlebar to hold the grip bars in a firm, stationary, and strong riding position. A single user gesture flips a clamp lever to loosen the split cylindrical clamp allows the grip bars to be slid outward and folded to a folded position. The split cylindrical clamp is then tightened with another single gesture, flipping the clamp lever in an opposite direction, locking the grip bars in the folded position. While locked in the folded position, the grip bars are entirely immobilized.

Abstract: The present invention relates to (a) variable regions of heavy and light chains of an antibody specific to a surface antigen in sporozoite of Eimeria spp.; (b) a recombinant scFV (single chain variable fragment) antibody prepared using the variable regions; (c) a method for preparing a recombinant scFv antibody; and (d) an expression vector for expressing a recombinant scFv antibody.

Abstract: The present invention provides a method of making a fluoropolymer comprising repeating units derived from one or more gaseous fluorinated monomers. The method comprises an aqueous emulsion polymerization of gaseous fluorinated monomers in the presence of an ether selected from the group consisting of dimethyl ether (DME), methyl tertiary butyl ether (MTBE) and mixtures thereof.

Abstract: Methods for improving sensitivity and specificity of polynucleotide synthesis are disclosed. The method includes reversibly blocking thermophilic polymerase activity with non-nucleic acid polyanions in a temperature dependent manner. The methods control target specific primer extension throughout all stages of a DNA or RNA amplification reaction. Corresponding compositions and kits are disclosed.

Abstract: Methods for improving sensitivity and specificity of polynucleotide synthesis are disclosed. The method includes reversibly blocking thermophilic polymerase activity with non-nucleic acid polyanions in a temperature dependent manner. The methods control target specific primer extension throughout all stages of a DNA or RNA amplification reaction. Corresponding compositions and kits are disclosed.

Abstract: A tetrafluoroethylene/hexafluoropropylene copolymer with high drawability is provided. Also provided is a process employing the polymer and an article coated with the polymer.

Abstract: A tetrafluoroethylene/hexafluoropropylene copolymer with high drawability is provided. Also provided is a process employing the polymer and an article coated with the polymer.

Abstract: The present invention relates to (a) variable regions of heavy and light chains of an antibody specific to a surface antigen in sporozoite of Eimeria spp.; (b) a recombinant scFV (single chain variable fragment) antibody prepared using the variable regions; (c) a method for preparing a recombinant scFv antibody; and (d) an expression vector for expressing a recombinant scFv antibody.