Abstract: A method for explosive cutting using converging shockwaves, and an explosive cutting device are disclosed. The method includes providing a projectile with an explosive charge, positioning the projectile over the object so it extends along an intended line of cut, and detonating the explosive charge so that the projectile is accelerated toward the object, wherein the projectile either impacts on the object and the projectile includes a wave-shaping element which is shaped such that the impact generates converging shockwaves in the underlying object to be cut causing a crack to be propagated through the object along the intended line of cut; or impacts on a wave-shaping element in contact with the object, the wave-shaping element being shaped such that the impact generates converging shockwaves in the underlying object causing a crack to be propagated through the object along the intended line of cut.

Abstract: A packer setting tool sets a liner top packer by mechanical rotation of the running tool and set down weight following cementing of a liner. The packer setting tool includes a tubular release body mounted on an end of the running tool. An annular dog sub circumscribes a portion of the release body. The dog sub is linked to the release body with a shear screw. A thread on an outer surface of the release body engages a thread on an inner surface of the dog sub to define a threaded connection between the dog sub and the release body. When the running tool rotates, the thread on the release body rotates with respect to the thread on the dog sub driving the release body in an axial direction fracturing the shear screw and urges an adapter sleeve against the packer assembly to set the packer assembly.

Abstract: The invention provides one-component and two-component DNA polymerases, as well as kits comprising the same, and methods of using the same for nucleic amplification and nucleic acid sequencing.

Abstract: The present invention relates to a thermophilic polymerase, wherein the DNA polymerase has an in-vitro primer extension rate that is >35 bases/second and faster relative to the primer extension rate of a DNA polymerase comprising amino acid sequences SEQ ID NO: 2 or 4, when measured under identical conditions in a DNA replication assay using primed single strand M13mp18 DNA and an incubation temperature of 60° C. The invention also relates to a vector comprising the polymerase, a host cell comprising the vector. The invention relates to a nucleic acid replication kit comprising the polymerase according to the invention.

Abstract: The invention relates to an electrical and electronic composite component (1), comprising a first joining partner (2) and at least one second joining partner (3). The invention provides for an openly porous sintered shaped part (6, 7) to be accommodated between the first and the second joining partners (2, 3), said sintered shaped part being sintered by sintering by means of sintering paste with the first and the second joining partners (2, 3). The invention furthermore relates to a production method.

Abstract: An electrical or electronic composite component is described as having a first joining partner and at least one second joining partner. According to the present system, it is provided that a sintered compact having open porosity is accommodated between the first and the second joining partner, the sintered compact is connected fixedly to the first and to the second joining partner.

Abstract: Improved chemodenervation agents are provided comprising polypeptide ?-neurotoxins having high binding specificity and selectivity for the human muscular ?1 nAChR instead of the human neuronal ?7 nAChR, along with pharmaceutical compositions and methods of use.

Abstract: Methods and compositions are provided for enhanced specificity and sensitivity of amplification reaction mixtures. Compositions in accordance with the present invention provide for reduced formation of primer-aggregates during amplification reactions. Reaction mixes include dNTPs, where a portion of the dNTPs has been replaced with an unconventional nucleotide, e.g., dUTP. Unconventional nucleotide concentrations are typically between 10% to 50% of the concentration of one of the standard dNTP. In some compositions the unconventional nucleotide is dUTP which replaces from about 10% to about 50% of the dTTP in the dNTP mix.

Abstract: The invention relates to an active enzyme of bacterial, fungal, viral, or archae origin, wherein the enzyme is coupled, preferably covalently coupled with at least one polymer having a molecular weight between about 500 to about 20,000 daltons selected from the group consisting of polyethylene glycol and polypropylene glycol. The invention relates in particular to uses thereof of such as in molecular biology as well as kits comprising such enzymes. In preferred embodiments the enzymes of the invention are nucleic acid modifying or replicating enzymes.

Abstract: In a method of manufacturing a multilayer device exposed surfaces of a first and second layer are joined together. Before joining a depression is created in the exposed surface of a layer and raw material, such as electrically conductive material, for forming an electric circuit is deposited in the depression, typically filling the depression. Excess material is mechanically removed before the layers are joined. In this way conductor tracks may be created between layers. When adjacent fluid chambers are provided, separated by one of the layers, the depression with the material in it makes it possible realize a sensor device with a reduced separation between the chambers.

Abstract: According to an aspect of the invention, there is provided an electrical package device comprising: a first substrate and a second substrate enclosing a first electric component; the second substrate supporting a second electric component, a plurality of connectors for mechanically connecting said first and second substrates in a stacked arrangement; and a seal provided between said first and second substrates at a distance from said first electric component; wherein said first electric component is electrically connected to said second electric component by connecting circuitry comprising said connectors; and wherein said connectors are provided in said seal. Preferably, said seal comprises a no flow resin material.

Abstract: The invention relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention also relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention further relates to a method for enzymatic nucleic acid synthesis comprising the steps of, (a) providing in a reaction mixture, a polymerase activity, a nucleic acid template, a zwitterionic detergent, a buffer, a salt, nucleotides and an inert protein and, (b) incubating the reaction mixture at a temperature which enables nucleic acid synthesis.

Abstract: In a method of manufacturing a multilayer device exposed surfaces of a first and second layer are forced together after applying adhesive. The first layer or stack of layers bent along its exposed surface and the exposed surface is brought into initial contact at a point or line of tangency along the bend. A temporally increasing force is exerted between the first and second layer through point or line of tangency, thereby reducing the resilient deformation of the first layer, until the bend has been forced out.

Abstract: Methods and compositions are provided for enhanced signal intensity and storage stability of standard nucleic acid amplification buffers, real-time PCR buffers or both. Buffers in accordance with the present invention include anti-freeze protein(s) (AFPs), optionally with a carrier protein, such as BSA.

Abstract: The invention relates to a method for armouring a window of an existing object comprising applying a transparent polymer layer to a surface of the window. The layer is usually applied to a surface facing the inside of the object. In particular, the invention may be employed to a conventional glazing of a building or vehicle.

Abstract: The present invention relates to a thermophilic polymerase, wherein the DNA polymerase has an in-vitro primer extension rate that is >35 bases/second and faster relative to the primer extension rate of a DNA polymerase comprising amino acid sequences SEQ ID NO: 2 or 4, when measured under identical conditions in a DNA replication assay using primed single strand M13mp18 DNA and an incubation temperature of 60° C. The invention also relates to a vector comprising the polymerase, a host cell comprising the vector. The invention relates to a nucleic acid replication kit comprising the polymerase according to the invention.

Abstract: The invention relates to the processing of a biological sample for histological analysis. In particular, it relates to a rapid automated processing system that can be operated with continuous throughput and that eliminates the use of toxic solvents such as xylene. Provided is a method for processing a biological sample for histological analysis, comprising contacting the sample with a composition comprising a supercritical or near supercritical fluid followed by impregnating the sample under a pressure of more than 1 bar with an embedding medium, preferably paraffin.

Abstract: The invention provides Pol III ? mutants, and modified Pol III replicases comprising the same, and methods of using modified Pol III replicases for a variety of nucleic acid replication reactions.

Abstract: The invention is directed to a process for the production of a jacketed penetrator having a tungsten, tungsten alloy or depleted uranium core and a metallic jacket, which process comprises placing the core inside the jacket and explosively welding the jacket on the core.

Abstract: A tetrafluoroethylene/hexafluoropropylene copolymer with high drawability is provided. Also provided is a process employing the polymer and an article coated with the polymer.