Patents by Inventor Eugeny A. Lukhtanov

Eugeny A. Lukhtanov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • NEGATIVELY CHARGED MINOR GROOVE BINDERS
    Publication number: 20100137614
    Abstract: The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.
    Type: Application
    Filed: August 14, 2009
    Publication date: June 3, 2010
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny A. Lukhtanov, Nicolaas M.J. Vermeulen, Alexander A. Gall
  • Phosphonate fluorescent dyes and conjugates
    Patent number: 7671218
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Grant
    Filed: August 12, 2005
    Date of Patent: March 2, 2010
    Assignee: Elitech Holdings B.V.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Compounds and methods for fluorescent labeling
    Patent number: 7601851
    Abstract: Dye reagents useful in labeling biological materials are provided along with methods for their use.
    Type: Grant
    Filed: May 10, 2005
    Date of Patent: October 13, 2009
    Assignee: Epoch Biosciences, Inc.
    Inventors: Eugeny A. Lukhtanov, Alexei V. Vorobiev, Michael W. Reed, Nicolaas M. J. Vermeulen
  • Negatively charged minor groove binders
    Patent number: 7582739
    Abstract: The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.
    Type: Grant
    Filed: March 11, 2003
    Date of Patent: September 1, 2009
    Assignee: Epoch Biosciences, Inc.
    Inventors: Eugeny A. Lukhtanov, Nicolaas M. J. Vermeulen, Alexander A. Gall
  • Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
    Patent number: 7556923
    Abstract: Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides.
    Type: Grant
    Filed: April 6, 2007
    Date of Patent: July 7, 2009
    Assignee: Epoch Biosciences, Inc.
    Inventors: Joel Hedgpeth, Irina A. Afonina, Igor V. Kutyavin, Eugeny A. Lukhtanov, Evgeniy S. Belousov, Rich B. Meyer, Jr.
  • Compounds and methods for fluorescent labeling
    Patent number: 7541454
    Abstract: Compounds useful in the fluorescent labeling of biological materials are provided along with methods for their use and preparation.
    Type: Grant
    Filed: October 25, 2004
    Date of Patent: June 2, 2009
    Assignee: Epoch Biosciences, Inc.
    Inventor: Eugeny A. Lukhtanov
  • HYBRIDIZATION AND MISMATCH DISCRIMINATION USING OLIGONUCLEOTIDES CONJUGATED TO MINOR GROOVE BINDERS
    Publication number: 20090048427
    Abstract: Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI3), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPI3 group resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides.
    Type: Application
    Filed: July 14, 2008
    Publication date: February 19, 2009
    Applicant: EPOCH BIOSCIENCES, INC.
    Inventors: JOEL HEDGPETH, IRINA A. AFONINA, IGOR V. KUTYAVIN, EUGENY A. LUKHTANOV, EVGENIY S. BELOUSOV, RICH B. MEYER, JR.
  • Real-time linear detection probes: sensitive 5'-minor groove binder-containing probes for PCR analysis
    Patent number: 7485442
    Abstract: Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5? nuclease digestion.
    Type: Grant
    Filed: June 19, 2006
    Date of Patent: February 3, 2009
    Assignee: Epoch Biosciences, Inc.
    Inventors: Irina A. Afonina, Yevgeniy S. Belousov, Robert O. Dempcy, Igor V. Kutyavin, Sergey G. Lokhov, Eugeny A. Lukhtanov
  • PHOSPHONYLATED FLUORESCENT DYES AND CONJUGATES
    Publication number: 20080207887
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Application
    Filed: November 8, 2007
    Publication date: August 28, 2008
    Applicant: EPOCH BIOSCIENCES, INC.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Phosphonate fluorescent dyes and conjugates
    Publication number: 20070172832
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Application
    Filed: August 12, 2005
    Publication date: July 26, 2007
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Real-time linear detection probes: sensitive 5?-minor groove binder-containing probes for PCR analysis
    Patent number: 7205105
    Abstract: Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5? nuclease digestion.
    Type: Grant
    Filed: June 6, 2002
    Date of Patent: April 17, 2007
    Assignee: Epoch Biosciences, Inc.
    Inventors: Irina A. Afonina, Yevgeniy S. Belousov, Robert O. Dempcy, Igor V. Kutyavin, Sergey G. Lokhov, Eugeny A. Lukhtanov
  • Real-time linear detection probes: sensitive 5'-minor groove binder-containing probes for PCR analysis
    Publication number: 20070003957
    Abstract: Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5? nuclease digestion.
    Type: Application
    Filed: June 19, 2006
    Publication date: January 4, 2007
    Applicant: Epoch Biosciences, Inc.
    Inventors: Irina Afonina, Yevgeniy Belousov, Robert Dempcy, Igor Kutyavin, Sergey Lokhov, Eugeny Lukhtanov
  • Fluorescent quenching detection reagents and methods
    Publication number: 20060292589
    Abstract: Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
    Type: Application
    Filed: January 23, 2006
    Publication date: December 28, 2006
    Applicant: Epoch Biosciences, Inc.
    Inventors: Michael Reed, Eugeny Lukhtanov, Alexander Gall, Robert Dempcy, Nicolaas Vermeulen
  • Compounds and methods for fluorescent labeling
    Patent number: 7112684
    Abstract: Dye reagents useful in labeling biological materials are provided along with methods for their use.
    Type: Grant
    Filed: December 22, 2004
    Date of Patent: September 26, 2006
    Assignee: Epoch Biosciences, Inc.
    Inventors: Eugeny A. Lukhtanov, Alexei V. Vorobiev, Michael W. Reed, Nicolaas M. J. Vermeulen
  • Compounds and methods for fluorescent labeling
    Publication number: 20060204990
    Abstract: Compounds useful in labeling biological materials are provided along with methods for their use.
    Type: Application
    Filed: February 17, 2006
    Publication date: September 14, 2006
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev, Michael Reed, Nicolaas Vermeulen
  • Phosphonylated fluorescent dyes and conjugates
    Publication number: 20060199955
    Abstract: Reagents are provided for the introduction of phosphonate groups into fluorescent dyes. Methods are also provided for preparing dye conjugates.
    Type: Application
    Filed: February 21, 2006
    Publication date: September 7, 2006
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev
  • Compounds and methods for fluorescent labeling
    Publication number: 20050277144
    Abstract: Dye reagents useful in labeling biological materials are provided along with methods for their use.
    Type: Application
    Filed: May 10, 2005
    Publication date: December 15, 2005
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev, Michael Reed, Nicolaas Vermeulen
  • Compounds and methods for fluorescent labeling
    Patent number: 6972339
    Abstract: Dye reagents useful in labeling biological materials are provided along with methods for their use.
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: December 6, 2005
    Assignee: Epoch Biosciences, Inc.
    Inventors: Eugeny A. Lukhtanov, Alexei V. Vorobiev, Michael W. Reed, Nicolaas M. J. Vermeulen
  • Negatively charged minor groove binders
    Publication number: 20050187383
    Abstract: The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.
    Type: Application
    Filed: March 11, 2003
    Publication date: August 25, 2005
    Inventors: Eugeny Lukhtanov, Sergey Lokhov, Nicolaas Vermeulen
  • Compounds and methods for fluorescent labeling
    Publication number: 20050176066
    Abstract: Dye reagents useful in labeling biological materials are provided along with methods for their use.
    Type: Application
    Filed: December 22, 2004
    Publication date: August 11, 2005
    Applicant: Epoch Biosciences, Inc.
    Inventors: Eugeny Lukhtanov, Alexei Vorobiev, Michael Reed, Nicolaas Vermeulen