Abstract: A bio-sheet applied on the eye tissue repair is disclosed. The bio-sheet comprises a biodegradable substrate formed of fish scales and has an epithelial cell layer formed thereon, wherein the fish scales are bony scales.

Abstract: Ophthalmic irrigating solutions are disclosed. The ophthalmic irrigating solution comprises: a) ?-polyglutamic acid (?-PGA) and/or salt thereof in an amount effective to increase the viscosity of the irrigating solution; and b) an ophthalmically acceptable aqueous vehicle for the ?-PGA and/or salt thereof. Also disclosed is a method of irrigating ocular tissues of a patient, in which the method comprises introducing to the ocular tissues of the patient an ophthalmic irrigating solution comprising ?-PGA) and/or salt thereof in an amount sufficient to irrigate the ocular tissues of the patient.

Abstract: A composition comprising a polymer that comprises oxidated hyaluronic acid cross-linked by a dihydrazide is disclosed. The polymer is a hydrogel exhibiting the following properties: a) transparent and colorless; and b) transforming from a liquid state into a gel-matrix at 37° C. These characteristics make it useful as a vitreous humor substitute.

Abstract: An implant containing a collagen matrix embedded with chondrocyte-like cells, its use in repairing a chondral defect, and a method of preparing the implant.

Abstract: The successful transfer of therapeutic agents such as genetic materials (e.g. nucleic acid) or drug into living cells is the most important issue depending on the development of the delivery carrier. A method for manufacturing superparamagnetic nanoparticles in medical therapeutics is described to develop nano-sized calcium phosphate (CaP) mineral was rendered magnetic as delivery vehicle. The CaP-based magnetized nanoparticles (NPs) were possessed superparamagnetic property by hetero-epitaxial growth of magnetite on the CaP crystallites and also showed no harm to the cultured cells and elicited no cytotoxicity. The magnetized CaP was demonstrated to have good plasmid DNA binding affinity or drug carrying capacity. It significantly increased the expression of gene transfection and efficiency in delivery to mesenchymal stem cells (MSCs) under exogenous magnetic field.

Abstract: In a long-lasting collagen and its manufacturing method, a pig skin is gone through processes of scraping extra tissues, removing fats, imbibition, digesting, centrifugal separation, salting-out, collecting lower-layer precipitate and freeze-drying to form a collagen, and the collagen is mixed with ?-PGA, and then a glutaraldehyde solution is added and mixed uniformly to perform a first crosslinking and form the long-lasting collagen, so as to overcome the shortcomings of a conventional collagen having a short storage time, a requirement of applying the collagen repeatedly, and a high concentration of remained glutaradldehyde which is biologically poisonous to human bodies.

Abstract: The present invention is related to an immuno-PCR method for detecting nasopharyngeal carcinoma (NPC) and kit thereof, especially related to an immuno-PCR method for detecting markers of early stage NPC and kit thereof. The present invention includes providing a substrate whereon protein markers immobilized; applying a patient's specimen to the substrate; adding a solution which has biotinylated anti-human IgA secondary antibody and incubating the solution; adding a solution with a linker and biotinylated target DNA; proceeding a polymerase chain reaction; and finally, detecting the target DNA fragments via electrophoresis.

Abstract: A pharmaceutical composition for inhalation delivery is provided, including drugs and a gelatin nanoparticle encapsulating the drugs to form a drug-gelatin nanocomplex, wherein the surface of the gelatin nanoparticle is modified with cell-targeting molecules. A method for fabricating the pharmaceutical composition for inhalation delivery is also provided.

Abstract: In a long-lasting collagen and its manufacturing method, a pig skin is gone through processes of scraping extra tissues, removing fats, imbibition, digesting, centrifugal separation, salting-out, collecting lower-layer precipitate and freeze-drying to form a collagen, and the collagen is mixed with ?-PGA, and then a glutaraldehyde solution is added and mixed uniformly to perform a first crosslinking and form the long-lasting collagen, so as to overcome the shortcomings of a conventional collagen having a short storage time, a requirement of applying the collagen repeatedly, and a high concentration of remained glutaradldehyde which is biologically poisonous to human bodies.

Abstract: The present invention discloses a cell culture device, including a container, and a carrying assembly; the container has an opening and holds the cell culture medium, and the carrying assembly is immersed in the cell culture solution from the opening of the container. The present invention also discloses a carrying assembly used for cell culture, including a cover, a supporter, and a vessel, wherein the cover and the vessel are connected by the supporter.

Abstract: A method for manufacturing the DP-bioglass to use in dental fracture repair via the carbon dioxide laser, the method comprises the steps: mixing and co-dissolving silicon and phosphoric acid raw material into ethanol; sequentially adding nitric acid, calcium and sodium raw material and stirring; standing, drying, triturating and heating the composition to obtain a glass powder material consisting of Na2O—CaO—SiO2—P2O5 and drying the oxide material mixing with a phosphoric acid to react the DP-bioglass.

Abstract: The present invention provides a biocompatible bilayer porous matrix and preparation thereof. The bilayer porous matrix is composed of gelatin, chondroitin 6 sulfate, and hyaluronic acid, also, prepared through freeze-drying technique at different temperature and time duration to form varied pore sizes on each layer. The present invention also provides a method of cell culture using the bilayer porous matrix.

Abstract: The present invention is related to an immuno-PCR method for detecting nasopharyngeal carcinoma (NPC) and kit thereof, especially related to an immuno-PCR method for detecting markers of early stage NPC and kit thereof. The present invention includes providing a substrate whereon protein markers immobilized; applying a patient's specimen to the substrate; adding a solution which has biotinylated anti-human IgA secondary antibody and incubating the solution; adding a solution with a linker and biotinylated target DNA; proceeding a polymerase chain reaction; and finally, detecting the target DNA fragments via electrophoresis.

Abstract: This invention is related to magnetic nanoparticles of hydroxyapatite for biomedical applications and the preparation method thereof. The magnetic particles of hydroxyapatite are prepared by a wet chemical co-precipitation method at lower temperature, wherein the calcium ions originally existing in hydroxyapatite are replaced with divalent or trivalent metal ions, for example, Fe+2 ion, to form magnetic nanoparticles of hydroxyapatite.

Abstract: A bio-degenerable bone cement is made of a mixture of poly (propylene fumarate) (PPF) and a diphasic material of tetra-calcium phosphate (TTCP)/dicalcium phosphate anhydrous (DCPA). The bone cement produced by a manufacturing method is injectable, bio-degenerable and non-penetrable to radioactive rays and features the advantages of a compressive strength close to the strength of bones, a low polymerization temperature, and a high biological compatibility.

Abstract: Bamboo vinegar is a natural product for sterilization and inhibition of bacteria growth and produces no pollution during the manufacturing process, and the discarded objects after the manufacturing process produce no pollution to the environment either. The bamboo vinegar added to shampoo for a pet's body and shampoo for a pet's hair have sterilization and antibacterial effects.

Abstract: A method for preparing porous bioceramic bone substitute materials is disclosed, which includes the following steps: (a) providing a cancellous bone of animals, (b) removing organic substances in the cancellous bone by thermal processing to obtain de-organic cancellous bone, (c) soaking the de-organic cancellous bone in a solution of phosphate salts, and (d) obtaining the porous bioceramic materials by sintering between 600 to 900° C. The porous bioceramic bone substitute materials of the present invention are suitable for use as filling materials of bone defect.

Abstract: A method for manufacturing the DP-bioglass to use in dental fracture repair via the carbon dioxide lasre, the method comprises the steps: using an oxide material including a sodium, calcium, silicon and phosphate dehydrogenase composition; triturating the oxide material and drying it; heating the oxide material and rapid freezing; and drying the oxide material mixing with a phosphoric acid to react the DP-bioglass.

Abstract: A biodegradable ceramic for medical use is disclosed, which comprises calcium hydrogenphosphate (CaHPO4) modified by organic molecules. The organic molecule mentioned above is hexamethylene diisocyanate (HMDI), which is grafted to the calcium hydrogenphosphate through covalent bond. The present invention also relates to a method for preparing the biodegradable ceramics. In addition, the biodegradable ceramic can be used for repair of bone defects. Therefore, the present invention further relates to a method and a device for repair of bone defects, and to a delivery system for the medicine of repair of bone defects.

Abstract: A dental retrograde-filling material process, comprising the steps of: taking 3:1 mole ratio of a calcium oxide and a silicon dioxide mixes a 1˜5 w.t. % cobalt oxide at 1200˜1800 degree Celsius temperature to get a A-product including a tricalcium silicate, a dicalcium silicate and a containing cobalt composition; taking 3:1 mole ratio of a calcium oxide and an aluminum oxide at 1200˜1800 degree Celsius temperature reacts a B-product including a tricalcium aluminate; taking 4:1:1 mole ratio of a calcium oxide, an aluminum oxide and a ferric oxide at 1200˜1800 degree Celsius temperature reacts a C-product including a calcium alminoferrite; and mixing the A-product, B-product and C-product are the retrograde-filling material. The dental retrograde-filling material mixes of taking 8:1:1 mole ratio of said tricalcium silicate, dicalcium silicate and containing cobalt composition, tricalcium aluminate and calcium alminoferrite.