Abstract: The present invention relates to a method for recording a transcriptome of a cell by: providing a test cell that includes a first transgene nucleic acid sequence encoding a fusion protein that is a reverse transcriptase polypeptide and a Cas1 polypeptide and a second transgene nucleic acid sequence encoding a Cas2 polypeptide, wherein the first transgene nucleic acid sequence and the second transgene nucleic acid sequence are under transcriptional control of an inducible promoter sequence, and a third transgene nucleic acid sequence including a CRISPR direct repeat (DR) sequence; wherein the CRISPR direct repeat sequence is specifically recognizable by a RT-Cas1-Cas2 complex formed by the expression products of the first transgene nucleic acid sequence and the second transgene nucleic acid sequence.

Abstract: A bearing arrangement includes a rotation-translation converter, a support component and an axial bearing with a single cage. The rotation-translation converter includes a first component that is axially positionally fixed and rotatable, and a second component that is axially movable by rotation of the first component. The axial bearing is for supporting the first component on the support component. The single cage includes a plurality of first pockets populated by first needles and having a first root circle diameter, and a plurality of second pockets, separate from the first pockets, populated by second needles and having a second root circle diameter that is greater than the first root circle diameter.

Abstract: The invention relates to a bacterial cell comprising a Cas1 RT fusion protein, a Cas2 protein and a CRISPR direct repeat (DR) sequence, wherein an RNA polymerase promoter in addition to the leader sequence is associated with the DR sequence. The invention further relates to a composition comprising two bacterial cell populations, each comprising a Cas1 RT fusion protein and Cas2 protein. The two cell types contain different versions of a CRISPR direct repeat (DR) sequence. The invention further relates to methods for analysis of transcription recording events of bacteria having passed through a subject's intestine, to assign a probability to the subject having a condition, such as malnutrition or inflammation of the intestine.

Abstract: The present application describes a computer-implemented method for identifying target-specific T cells and their T cell Receptor (TCR) sequences. The method includes deriving single cell T cell data from a sample. The data comprises T cell profile and T cell TCR sequence. The method also includes selecting candidate T cells and their TCR sequences from the single cell T cell data using a machine learning classifier that is trained to classify T cells based on their profiles. The method may also include aggregating results over clonotypes, adding T cells with similar TCR sequences and ranking the list of candidates.

Abstract: The invention relates to a method for solvent-free production of an electrode. By means of the method it is possible to produce an electrode which has high mechanical stability and which provides a high specific capacitance. The method also enables the production of an electrode which can be used as an anode in a lithium-ion battery, without giving rise to unstable operation with undesirable and irreversible degradation reactions. An electrode is also provided, which has the above-mentioned advantages. In addition, uses of the electrode according to the invention are proposed.

Abstract: A component system for an aircraft, having at least one furnishing component, a plurality of component rails, a plurality of guide elements engageable with and movable relative to the component rails, a plurality of retaining elements connected to the guide elements and configured to be coupled to a hull structure of the aircraft or the at least one furnishing component, and a plurality of locking units arranged on the component rails and/or on the guide elements and configured to lock the guide elements to a particular rail. At least two component rails are arranged on the at least one furnishing component or the hull structure at a distance and parallel to each other such that the furnishing component is retractable via the component rails on guide elements or component rails that are spatially fixed in the aircraft and can be locked in a retracted position by the locking units.

Abstract: A device (1) for connecting a toothing part (3) to a shaft (5) for conjoint rotation with the latter, having a toothing part (3) with a circular bore, a shaft (5) which has: a cylindrical receiving portion (4) for receiving the toothing part (3), and a cylindrical recess (8) in the shaft (5), which cylindrical recess (8) extends at least substantially over the entire length of the receiving portion (4), and a press-in cylinder (10) which can be pressed into the recess (8) and which has an oversize in relation to the recess (8).

Abstract: The invention relates to an additive manufacturing method in which a component (10, 42, 43, 44, 45) is produced in layers using an energy beam (8, 41, 58) which solidifies a starting material (4) and is irradiated by energy beam irradiating means (9, 22, 31, 38, 39, 55, 59, 61) while the starting material (4) is held by a base surface (3, 15, 30, 36, 52) arranged on a base element (2, 16, 29, 35, 51). While the starting material (4) is being irradiated with the energy beam (8, 41, 58), the base element (2, 16, 29, 35, 51) is moved by a rotational component which has a base element rotational axis, wherein the starting material (4) is held on the base surface (3, 15, 30, 36, 52) by a centrifugal acceleration generated by the rotational component. The invention is characterized in that a rotational movement is produced for at least some of the energy beam irradiating means (9, 22, 31, 38, 39, 55, 59, 61).

Abstract: The invention relates to an additive manufacturing method in which a component (10, 42, 43, 44, 45) is produced in layers using an energy beam (8, 41, 58) which solidifies a starting material (4) and is irradiated by energy beam irradiating means (9, 22, 31, 38, 39, 55, 59, 61) while the starting material (4) is held by a base surface (3, 15, 30, 36, 52) arranged on a base element (2, 16, 29, 35, 51). While the starting material (4) is being irradiated with the energy beam (8, 41, 58), the base element (2, 16, 29, 35, 51) is moved by a rotational component which has a base element rotational axis, wherein the starting material (4) is held on the base surface (3, 15, 30, 36, 52) by a centrifugal acceleration generated by the rotational component. The invention is characterized in that a rotational movement is produced for at least some of the energy beam irradiating means (9, 22, 31, 38, 39, 55, 59, 61).

Abstract: A component system for an aircraft, having at least one furnishing component, a plurality of component rails, a plurality of guide elements engageable with and movable relative to the component rails, a plurality of retaining elements connected to the guide elements and configured to be coupled to a hull structure of the aircraft or the at least one furnishing component, and a plurality of locking units arranged on the component rails and/or on the guide elements and configured to lock the guide elements to a particular rail. At least two component rails are arranged on the at least one furnishing component or the hull structure at a distance and parallel to each other such that the furnishing component is retractable via the component rails on guide elements or component rails that are spatially fixed in the aircraft and can be locked in a retracted position by the locking units.

Abstract: The invention relates to a battery box for accommodating at least one battery for an electric vehicle, having an outer circumferential frame and a base. Below the frame, a circumferential frame reinforcing element is arranged for reinforcing the frame, wherein the material thickness of the frame reinforcing element corresponds to its cross-sectional height.

Abstract: The present invention relates to a method for recording a transcriptome of a cell, the method comprising the steps of: providing a test cell comprising: a first transgene nucleic acid sequence encoding a fusion protein comprising a reverse transcriptase polypeptide and a Cas1 polypeptide and a second transgene nucleic acid sequence encoding a Cas2 polypeptide, wherein said first transgene nucleic acid sequence and said second transgene nucleic acid sequence are under transcriptional control of an inducible promoter sequence, and a third transgene nucleic acid sequence comprising a CRISPR direct repeat (DR) sequence; wherein said CRISPR direct repeat sequence is specifically recognizable by a RT-Cas1-Cas2 complex formed by the expression products of said first transgene nucleic acid sequence and said second transgene nucleic acid sequence, exposing said test cell to conditions under which expression of said first transgene nucleic acid sequence and said second transgene nucleic acid sequence is induced, wherei

Abstract: A method for allocating a workload to a cluster machine of a plurality of cluster machines which are part of a computer cluster operating in a cluster computing environment, includes the step of collecting values from hardware performance counters of each of the cluster machines while the cluster machines are running different workloads. A value of a hardware performance counter from a system which executed the workload to be allocated in isolation and the values from the hardware performance counters of each of the cluster machines which are running the different workloads are used as input to a machine learning algorithm trained to provide as output in each case a prediction of a performance of the workload on each of the cluster machines which are running the different workloads. The cluster machine is selected for placement of the workload based on the predictions.

Abstract: Device (1) for connecting a toothing part (3) to a shaft (5) for conjoint rotation with the latter, having a toothing part (3) with a circular bore, a shaft (5) which has: a cylindrical receiving portion (4) for receiving the toothing part (3), and a cylindrical recess (8) in the shaft (5), which cylindrical recess (8) extends at least substantially over the entire length of the receiving portion (4), and a press-in cylinder (10) which can be pressed into the recess (8) and which has an oversize in relation to the recess (8).

Abstract: A method for monitoring resources in a computing system having system information includes transforming, via representation learning, variable-size information into fixed-size information, and creating a machine learning neural network model and training it the machine learning model to predict future resource usage of an application. The method further includes providing the prediction of further resources usage of the application as an input to an action component, wherein the action component is one of an anomaly detector or a reinforcement learner that drives a scheduler. The method additionally includes performing, by the action component, at least one of scheduling resources within the computing system or detecting a resources usage anomaly.

Abstract: A method for operating virtual machines on a virtualization platform includes: embedding control information in a predetermined memory area of a front-end virtual machine where at least one virtual device is to be initialized, the control information being required for initiating a communication with a back-end virtual machine where at least one back-end driver runs; retrieving, by the front-end virtual machine, the control information from the predetermined memory area of the front-end virtual machine; and performing the communication between the front-end virtual machine and the back-end virtual machine via a direct communication channel to exchange information for initializing the at least one virtual device of the front-end virtual machine, by communicating with the at least one back-end driver via the direct communication channel. The direct communication channel is established based on the control information embedded in the predetermined memory area of the front-end virtual machine.

Abstract: A method for operating virtual machines on a virtualization platform, the method comprising: embedding, preferably by a toolstack of the virtualization platform, control information in a predetermined memory area of a front-end virtual machine where at least one virtual device is to be initialized, wherein said control information is required for initiating a communication with a back-end virtual machine where at least one back-end driver runs; retrieving, by the front-end virtual machine, said control information from said predetermined memory area of the front-end virtual machine; and performing the communication between the front-end virtual machine and the back-end virtual machine via a direct communication channel in order to exchange information for initializing said at least one virtual device of the front-end virtual machine, in particular by communicating with said at least one back-end driver via said direct communication channel, wherein said direct communication channel is established based on sai

Abstract: A method for monitoring resources in a computing system having system information includes transforming, via representation learning, variable-size information into fixed-size information, and creating a machine learning neural network model and training it the machine learning model to predict future resource usage of an application. The method further includes providing the prediction of further resources usage of the application as an input to an action component, wherein the action component is one of an anomaly detector or a reinforcement learner that drives a scheduler. The method additionally includes performing, by the action component, at least one of scheduling resources within the computing system or detecting a resources usage anomaly.

Abstract: A corrugated fin, in particular for a heat exchanger, having a substantially corrugated design, having a plurality of fin surfaces, wherein adjoining fin surfaces are connected to one another by means of a fin arch in such a way that a medium can flow through an intermediate space between adjoining fin surfaces, wherein the fin surfaces are arranged at an angle with respect to one another perpendicularly with respect to a throughflow direction for the medium, wherein the fin surface has at least one bulge which protrudes out of the plane of the fin surface, wherein the extent of the bulge in a direction perpendicularly with respect to the air throughflow direction is smaller than the extent of the fin surface in said direction.

Abstract: The present disclosure includes an insert for a fuse structure. In one approach, a fuse, includes a housing having a cavity, a fuse element disposed within the cavity, a plurality of terminals extending out of the housing and electrically connected to the fuse element, and an insert disposed in the cavity, the insert including a pin extending through an opening of the housing. In some approaches, the insert includes a separating wall defining first and second cavities of the insert.