Patents by Inventor Frances H. Arnold

Frances H. Arnold has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10934531
    Abstract: Methods for catalyzing C—H insertion reactions using heme enzymes are described. The present disclosure provides a method for producing a C—H insertion product comprising providing an substrate having an sp3-hybridized C—H bond, a carbene precursor such as a diazo reagent, and a heme enzyme, and admixing the components in a reaction for a time sufficient to produce the C—H insertion product. Heme enzyme variants useful for carrying out in vivo and in vitro C—H insertion reactions, as well as expression vectors and host cells expressing the heme enzymes, are also described.
    Type: Grant
    Filed: January 24, 2019
    Date of Patent: March 2, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Ruijie Zhang, Lena Wohlschlager, Hans H. Renata, Frances H. Arnold, Kai Chen
  • Patent number: 10927355
    Abstract: The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.
    Type: Grant
    Filed: September 5, 2018
    Date of Patent: February 23, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Sek Bik Jennifer Kan, Russell D. Lewis, Kai Chen, Frances H. Arnold
  • Publication number: 20200087358
    Abstract: Disclosed herein are engineered light-sensitive proteins, for example channelrhodopsins and variants thereof. Also disclosed are compositions for expressing the light-sensitive proteins in cells, tissues, organs and subjects, and methods for using the light-sensitive proteins to, for example, enable minimally-invasive neuronal circuit interrogation in living organism, and treat neuronal and ocular disorders.
    Type: Application
    Filed: September 17, 2019
    Publication date: March 19, 2020
    Inventors: Viviana Gradinaru, Claire N. Bedbrook, Frances H. Arnold, Kevin K. Yang
  • Patent number: 10501762
    Abstract: The disclosure generally relates to the fields of synthetic organic chemistry. In particular, the present disclosure relates to methods and systems for the imidation of sulfides.
    Type: Grant
    Filed: July 12, 2016
    Date of Patent: December 10, 2019
    Assignee: California Institute of Technology
    Inventors: Christopher C. Farwell, John A. McIntosh, Frances H. Arnold
  • Publication number: 20190276805
    Abstract: Methods for catalyzing C—H insertion reactions using heme enzymes are described. The present disclosure provides a method for producing a C—H insertion product comprising providing an substrate having an sp3-hybridized C—H bond, a carbene precursor such as a diazo reagent, and a heme enzyme, and admixing the components in a reaction for a time sufficient to produce the C—H insertion product. Heme enzyme variants useful for carrying out in vivo and in vitro C—H insertion reactions, as well as expression vectors and host cells expressing the heme enzymes, are also described.
    Type: Application
    Filed: January 24, 2019
    Publication date: September 12, 2019
    Applicant: California Institute of Technology
    Inventors: Ruijie Zhang, Lena Wohlschlager, Hans H. Renata, Frances H. Arnold, Kai Chen
  • Publication number: 20190211367
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Application
    Filed: January 11, 2019
    Publication date: July 11, 2019
    Applicant: California Institute of Technology
    Inventors: Pedro S. Coelho, Eric M. Burstad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Patent number: 10208322
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Grant
    Filed: September 28, 2016
    Date of Patent: February 19, 2019
    Assignee: California Institute of Technology
    Inventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Publication number: 20180371430
    Abstract: The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.
    Type: Application
    Filed: September 5, 2018
    Publication date: December 27, 2018
    Applicant: California Institute of Technology
    Inventors: Sek Bik Jennifer Kan, Russell D. Lewis, Kai Chen, Frances H. Arnold
  • Patent number: 10093906
    Abstract: The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: October 9, 2018
    Assignee: California Institute of Technology
    Inventors: Sek Bik Jennifer Kan, Russell D. Lewis, Kai Chen, Frances H. Arnold
  • Publication number: 20170247725
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Application
    Filed: September 28, 2016
    Publication date: August 31, 2017
    Applicant: California Institute of Technology
    Inventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Publication number: 20170218346
    Abstract: The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.
    Type: Application
    Filed: February 1, 2017
    Publication date: August 3, 2017
    Applicant: California Institute of Technology
    Inventors: Sek Bik Jennifer Kan, Russell D. Lewis, Kai Chen, Frances H. Arnold
  • Patent number: 9708633
    Abstract: The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: July 18, 2017
    Assignee: California Institute of Technology
    Inventors: Frances H. Arnold, Pete Heinzelman
  • Publication number: 20170037441
    Abstract: The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
    Type: Application
    Filed: March 11, 2016
    Publication date: February 9, 2017
    Inventors: Frances H. Arnold, Pete Heinzelman
  • Publication number: 20160355852
    Abstract: The disclosure generally relates to the fields of synthetic organic chemistry. In particular, the present disclosure relates to methods and systems for the imidation of sulfides.
    Type: Application
    Filed: July 12, 2016
    Publication date: December 8, 2016
    Inventors: Christopher C. Farwell, John A. McIntosh, Frances H. Arnold
  • Publication number: 20160355799
    Abstract: The present disclosure relates to CBH II chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
    Type: Application
    Filed: January 25, 2016
    Publication date: December 8, 2016
    Inventors: Frances H. Arnold, Pete Heinzelman
  • Patent number: 9493799
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: November 15, 2016
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Publication number: 20160251642
    Abstract: The disclosure provides variant Ce16a enzymes having increased thermostability, methods of making and using such polypeptides.
    Type: Application
    Filed: April 25, 2016
    Publication date: September 1, 2016
    Inventors: Frances H. Arnold, Indira Wu
  • Patent number: 9399762
    Abstract: The disclosure generally relates to the fields of synthetic organic chemistry. In particular, the present disclosure relates to methods and systems for the imidation of sulfides.
    Type: Grant
    Filed: February 18, 2015
    Date of Patent: July 26, 2016
    Assignee: California Institute of Technology
    Inventors: Christopher C. Farwell, John A. McIntosh, Frances H. Arnold
  • Patent number: 9334517
    Abstract: A variant Cel5a endoglucanase has increased thermostability, increased enzymatic activity and/or increased expression in a host, relative to wild type Cel5a. The improved variant Cel5a endoglucanase may be used to hydrolyze more cellulose at a higher temperature for a more efficient and cost-effective production of biofuels as compared to wild type Cel5a.
    Type: Grant
    Filed: April 7, 2014
    Date of Patent: May 10, 2016
    Assignee: California Institute of Technology
    Inventors: Toni M. Lee, Stephen L. Mayo, Frances H. Arnold, Devin Trudeau
  • Patent number: 9334544
    Abstract: A variant Cel5a endoglucanase has increased thermostability, increased enzymatic activity and/or increased expression in a host, relative to wild type Cel5a. The improved variant Cel5a endoglucanase may be used to hydrolyze more cellulose at a higher temperature for a more efficient and cost-effective production of biofuels as compared to wild type Cel5a. A variant Cel5a endoglucanase is combined with variant Cel6a and variant Cel7a cellobiohydrolases resulting in more effective hydrolysis of cellulose.
    Type: Grant
    Filed: October 16, 2014
    Date of Patent: May 10, 2016
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Devin L. Trudeau, Frances H. Arnold, Toni M. Lee, Stephen L. Mayo