Patents by Inventor Frances H. Arnold

Frances H. Arnold has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240166700
    Abstract: Disclosed herein are engineered light-sensitive proteins, for example channelrhodopsins and variants thereof. Also disclosed are compositions for expressing the light-sensitive proteins in cells, tissues, organs and subjects, and methods for using the light-sensitive proteins to, for example, enable minimally-invasive neuronal circuit interrogation in living organism, and treat neuronal and ocular disorders.
    Type: Application
    Filed: November 22, 2023
    Publication date: May 23, 2024
    Inventors: Viviana Gradinaru, Claire N. Bedbrook, Frances H. Arnold, Kevin K. Yang
  • Patent number: 11858969
    Abstract: Disclosed herein are engineered light-sensitive proteins, for example channelrhodopsins and variants thereof. Also disclosed are compositions for expressing the light-sensitive proteins in cells, tissues, organs and subjects, and methods for using the light-sensitive proteins to, for example, enable minimally-invasive neuronal circuit interrogation in living organism, and treat neuronal and ocular disorders.
    Type: Grant
    Filed: September 17, 2019
    Date of Patent: January 2, 2024
    Assignee: California Institute of Technology
    Inventors: Viviana Gradinaru, Claire N. Bedbrook, Frances H. Arnold, Kevin K. Yang
  • Publication number: 20230313245
    Abstract: The present disclosure provides engineered enzymes and methods for preparing tyrosine and tyrosine analogs from a donor amino acid and phenols in a single step. Also disclosed are methods to engineer enzymes having such ‘tyrosine synthase’ activity, starting from the beta-subunit of a tryptophan synthase (TrpB).
    Type: Application
    Filed: November 10, 2022
    Publication date: October 5, 2023
    Applicant: California Institute of Technology
    Inventors: Patrick J. Almhjell, Frances H. Arnold
  • Publication number: 20230102728
    Abstract: This disclosure provides a method of preparing a silanol-functional organosilicon compound with a cytochrome P450 variant that facilitates the oxidization of a silyl hydride group to a silanol group in the presence of oxygen. The method includes combining the cytochrome P450 variant and an organosilicon compound having at least one silicon-bonded hydrogen atom to give a reaction mixture and exposing the reaction mixture to oxygen to oxidize the organosilicon compound, thereby preparing the silanol-functional organosilicon compound. Cytochrome P450 variants suitable for use in the method are also disclosed, along with methods for engineering and optimizing the same. Nucleic acids encoding the cytochrome P450 variants and compositions, expression vectors, and host cells including the same are also disclosed.
    Type: Application
    Filed: February 19, 2021
    Publication date: March 30, 2023
    Inventors: John ROBERTS, Dimitris Elias KATSOULIS, Susanne BAEHR, Sabine BRINKMANN-CHEN, S.B. Jennifer KAN, Frances H. ARNOLD
  • Patent number: 11008596
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Grant
    Filed: January 11, 2019
    Date of Patent: May 18, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Patent number: 10934531
    Abstract: Methods for catalyzing C—H insertion reactions using heme enzymes are described. The present disclosure provides a method for producing a C—H insertion product comprising providing an substrate having an sp3-hybridized C—H bond, a carbene precursor such as a diazo reagent, and a heme enzyme, and admixing the components in a reaction for a time sufficient to produce the C—H insertion product. Heme enzyme variants useful for carrying out in vivo and in vitro C—H insertion reactions, as well as expression vectors and host cells expressing the heme enzymes, are also described.
    Type: Grant
    Filed: January 24, 2019
    Date of Patent: March 2, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Ruijie Zhang, Lena Wohlschlager, Hans H. Renata, Frances H. Arnold, Kai Chen
  • Patent number: 10927355
    Abstract: The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.
    Type: Grant
    Filed: September 5, 2018
    Date of Patent: February 23, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Sek Bik Jennifer Kan, Russell D. Lewis, Kai Chen, Frances H. Arnold
  • Publication number: 20200087358
    Abstract: Disclosed herein are engineered light-sensitive proteins, for example channelrhodopsins and variants thereof. Also disclosed are compositions for expressing the light-sensitive proteins in cells, tissues, organs and subjects, and methods for using the light-sensitive proteins to, for example, enable minimally-invasive neuronal circuit interrogation in living organism, and treat neuronal and ocular disorders.
    Type: Application
    Filed: September 17, 2019
    Publication date: March 19, 2020
    Inventors: Viviana Gradinaru, Claire N. Bedbrook, Frances H. Arnold, Kevin K. Yang
  • Patent number: 10501762
    Abstract: The disclosure generally relates to the fields of synthetic organic chemistry. In particular, the present disclosure relates to methods and systems for the imidation of sulfides.
    Type: Grant
    Filed: July 12, 2016
    Date of Patent: December 10, 2019
    Assignee: California Institute of Technology
    Inventors: Christopher C. Farwell, John A. McIntosh, Frances H. Arnold
  • Publication number: 20190276805
    Abstract: Methods for catalyzing C—H insertion reactions using heme enzymes are described. The present disclosure provides a method for producing a C—H insertion product comprising providing an substrate having an sp3-hybridized C—H bond, a carbene precursor such as a diazo reagent, and a heme enzyme, and admixing the components in a reaction for a time sufficient to produce the C—H insertion product. Heme enzyme variants useful for carrying out in vivo and in vitro C—H insertion reactions, as well as expression vectors and host cells expressing the heme enzymes, are also described.
    Type: Application
    Filed: January 24, 2019
    Publication date: September 12, 2019
    Applicant: California Institute of Technology
    Inventors: Ruijie Zhang, Lena Wohlschlager, Hans H. Renata, Frances H. Arnold, Kai Chen
  • Publication number: 20190211367
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Application
    Filed: January 11, 2019
    Publication date: July 11, 2019
    Applicant: California Institute of Technology
    Inventors: Pedro S. Coelho, Eric M. Burstad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Patent number: 10208322
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Grant
    Filed: September 28, 2016
    Date of Patent: February 19, 2019
    Assignee: California Institute of Technology
    Inventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Publication number: 20180371430
    Abstract: The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.
    Type: Application
    Filed: September 5, 2018
    Publication date: December 27, 2018
    Applicant: California Institute of Technology
    Inventors: Sek Bik Jennifer Kan, Russell D. Lewis, Kai Chen, Frances H. Arnold
  • Patent number: 10093906
    Abstract: The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: October 9, 2018
    Assignee: California Institute of Technology
    Inventors: Sek Bik Jennifer Kan, Russell D. Lewis, Kai Chen, Frances H. Arnold
  • Publication number: 20170247725
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Application
    Filed: September 28, 2016
    Publication date: August 31, 2017
    Applicant: California Institute of Technology
    Inventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Publication number: 20170218346
    Abstract: The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.
    Type: Application
    Filed: February 1, 2017
    Publication date: August 3, 2017
    Applicant: California Institute of Technology
    Inventors: Sek Bik Jennifer Kan, Russell D. Lewis, Kai Chen, Frances H. Arnold
  • Patent number: 9708633
    Abstract: The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: July 18, 2017
    Assignee: California Institute of Technology
    Inventors: Frances H. Arnold, Pete Heinzelman
  • Publication number: 20170037441
    Abstract: The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
    Type: Application
    Filed: March 11, 2016
    Publication date: February 9, 2017
    Inventors: Frances H. Arnold, Pete Heinzelman
  • Publication number: 20160355799
    Abstract: The present disclosure relates to CBH II chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
    Type: Application
    Filed: January 25, 2016
    Publication date: December 8, 2016
    Inventors: Frances H. Arnold, Pete Heinzelman
  • Publication number: 20160355852
    Abstract: The disclosure generally relates to the fields of synthetic organic chemistry. In particular, the present disclosure relates to methods and systems for the imidation of sulfides.
    Type: Application
    Filed: July 12, 2016
    Publication date: December 8, 2016
    Inventors: Christopher C. Farwell, John A. McIntosh, Frances H. Arnold