Patents by Inventor Frances H. Arnold
Frances H. Arnold has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20100255542Abstract: The present disclosure relates to CBH II chimera fusion polypetides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.Type: ApplicationFiled: April 6, 2010Publication date: October 7, 2010Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Frances H. Arnold, Pete Heinzelman
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Publication number: 20100248327Abstract: Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.Type: ApplicationFiled: April 5, 2010Publication date: September 30, 2010Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Edgardo T. Farinas, Frances H. Arnold, Ulrich Schwaneberg, Anton Glieder
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Patent number: 7704715Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions.Type: GrantFiled: October 11, 2008Date of Patent: April 27, 2010Assignee: California Institute of TechnologyInventors: Patrick C. Cirino, Frances H. Arnold
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Patent number: 7691616Abstract: Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.Type: GrantFiled: May 7, 2007Date of Patent: April 6, 2010Assignee: California Institute of TechnologyInventors: Edgardo T. Farinas, Frances H. Arnold, Ulrich Schwaneberg, Anton Glieder
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Publication number: 20090298148Abstract: Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-positio to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio-and enantio-selectivities are retained in whole-cell biotransformations with E. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.Type: ApplicationFiled: April 15, 2009Publication date: December 3, 2009Applicant: THE CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Frances H. Arnold, Matthew W. Peters, Peter Meinhold
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Publication number: 20090264311Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A/V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and/or V446I amino acid substitutions.Type: ApplicationFiled: October 11, 2008Publication date: October 22, 2009Applicant: The California Institute of TechnologyInventors: Frances H. Arnold, Patrick C. Cirino
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Publication number: 20090209010Abstract: A method and system for selectively fluorinating organic molecules on a target site wherein the target site is activated and then fluorinated are shown together with a method and system for identifying a molecule having a biological activity.Type: ApplicationFiled: February 4, 2009Publication date: August 20, 2009Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Rudi Fasan, Frances H. Arnold
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Publication number: 20090142821Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having 158V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L3241, I366V, K434E, E442K, and/or V446I amino acid substitutions.Type: ApplicationFiled: October 11, 2008Publication date: June 4, 2009Applicant: The California Institute of TechnologyInventors: Patrick C. Cirino, Frances H. Arnold
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Publication number: 20090124515Abstract: The disclosure relates to engineered P450 polypeptides and use of such polypeptides in chemoenzymatic methods to construct selectively protected carbohydrates, which are useful as building blocks for preparation of carbohydrate derivatives and oligosaccharidesType: ApplicationFiled: June 18, 2008Publication date: May 14, 2009Applicant: The California Institute of TechnologyInventors: Frances H. Arnold, Chi-Huey Wong, Yuuichi Mitsuda, Michael M. Chen, Clay Bennett, William Greenberg, Jared Crawford Lewis, Sabine Bastian
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Patent number: 7524664Abstract: Cytochrome P450 BM-3 from Bacillus megaterium was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-positio to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with E. coli, where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.Type: GrantFiled: June 15, 2004Date of Patent: April 28, 2009Assignee: California Institute of TechnologyInventors: Frances H. Arnold, Matthew W. Peters, Peter Meinhold
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Publication number: 20090061471Abstract: A method and system for selectively fluorinating organic molecules on a target site wherein the target site is activated and then fluorinated are shown together with a method and system for identifying a molecule having a biological activity.Type: ApplicationFiled: August 4, 2007Publication date: March 5, 2009Inventors: Rudi Fasan, Frances H. Arnold
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Patent number: 7465567Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions.Type: GrantFiled: April 16, 2002Date of Patent: December 16, 2008Assignee: California Institute of TechnologyInventors: Patrick C. Cirino, Frances H. Arnold
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Publication number: 20080293928Abstract: Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.Type: ApplicationFiled: May 7, 2007Publication date: November 27, 2008Inventors: Edgardo T. Farinas, Frances H. Arnold, Ulrich Schwaneberg, Anton Glieder
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Publication number: 20080268517Abstract: The present disclosure relates to cytochrome p450 fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.Type: ApplicationFiled: March 15, 2008Publication date: October 30, 2008Applicant: THE CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Frances H. Arnold, Yougen Li
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Patent number: 7435570Abstract: The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A/V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and/or V446I amino acid substitutions.Type: GrantFiled: August 11, 2004Date of Patent: October 14, 2008Assignee: California Institute of TechnologyInventors: Frances H. Arnold, Patrick C. Cirino
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Publication number: 20080248545Abstract: The disclosure provides methods for identifying and producing stabilized chimeric proteins.Type: ApplicationFiled: February 1, 2008Publication date: October 9, 2008Applicant: THE CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Frances H. Arnold, Yougen Li
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Publication number: 20080176211Abstract: The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time.Type: ApplicationFiled: May 3, 2007Publication date: July 24, 2008Applicant: California Institute of TechnologyInventors: Charles F. Spence, Anne Y. Fu, Stephen R. Quake, Frances H. Arnold
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Patent number: 7226768Abstract: Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3.Type: GrantFiled: July 22, 2002Date of Patent: June 5, 2007Assignee: The California Institute of TechnologyInventors: Edgardo T. Farinas, Frances H. Arnold, Ulrich Schwaneberg, Anton Glieder
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Patent number: 7220563Abstract: Glucose oxidase enzymes are provided, including novel variants of galactose oxidase enzymes. The polynucleotides that encode these novel variants can be expressed in recombinant host cell expression systems. The novel variant oxidase enzymes are capable of oxidizing compounds towards which wild-type galactose oxidase (e.g. D-galactose: oxygen 6-oxidoreductase, GAO; EC 1.1.3.9) has little or no activity. Preferred galactose oxidase variants are those which that have improved capability to oxidize secondary alcohols and/or D-glucose relative to the wild-type enzyme.Type: GrantFiled: February 27, 2003Date of Patent: May 22, 2007Assignee: California Institute of TechnologyInventors: Frances H. Arnold, Lian-Hong Sun, Ioanna P. Petrounia
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Patent number: 7214298Abstract: The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time.Type: GrantFiled: August 13, 2001Date of Patent: May 8, 2007Assignee: California Institute of TechnologyInventors: Charles F. Spence, Anne Y. Fu, Stephen R. Quake, Frances H. Arnold