Abstract: An microheater array system includes an integrated microheater array configured to generate a localized heat and having a plurality of pixels. Each pixel includes: an inductor; a stacked oscillator configured to generate a magnetic field at microwave frequencies with tunable intensity and frequency; and an electro-thermal loop. The microheater array system may further include a plurality of magnetic nanoparticles (MNPs).

Abstract: Methods and systems for searching genomes for potential nucleotide-guided nuclease off-target sites are provided. Also provided are methods of searching genomes for potential off-target deadCas9 binding sites. In some embodiments, the methods include ranking the potential off-target sites based on the number and location of mismatches, insertions, and/or deletions in the DNA, RNA, or DNA/RNA guide sequence relative to the genomic DNA sequence at a putative target site in the genome, allowing the selection of better target sites and/or experimental confirmation of off-target sites.

Abstract: A method, an apparatus, and a computer program product for wireless communication are provided. The apparatus may be a UE. The UE determines whether the UE is located within a sub-region of an MBSFN area based on one or more parameters. The UE receives based on the determination a service over broadcast in the sub-region of the MBSFN area or over unicast outside the sub-region. In an aspect, the MBSFN area may be smaller than a unicast area. In an aspect, adaptive retransmission with a group NACK approach may be used to improve an SNR and reliability.

Abstract: Endonucleases play an essential role in genetic engineering and molecular biology. A major barrier to the clinical adoption of nucleases and engineered nucleases remain the overall lack of specificity and activity. Off-site cleavage, cleavage at loci other than the target loci, typically occurs resulting in mutations, unexpected gene-knockouts, or translocations. Provided herein are systems and methods for identifying the off-site cleavage loci and predicting the activity of engineered endonucleases for a given genome. It is expected that these tools and methods will be useful for designing nucleases and other related DNA binding domains (e.g. TAL effectors) for genomic therapy and engineering.

Abstract: A novel fluorophore based on an acid soluble reagent that is a lanthanide metal ion nanoparticle that is passivated and/or coated for water dispersion and to minimize nonspecific binding, and assays for same

Abstract: Methods and systems for searching genomes for potential nucleotide-guided nuclease off-target sites are provided. Also provided are methods of searching genomes for potential off-target deadCas9 binding sites. In some embodiments, the methods include ranking the potential off-target sites based on the number and location of mismatches, insertions, and/or deletions in the DNA, RNA, or DNA/RNA guide sequence relative to the genomic DNA sequence at a putative target site in the genome, allowing the selection of better target sites and/or experimental confirmation of off-target sites.

Abstract: A method, an apparatus, and a computer program product for wireless communication are provided. The apparatus may be a UE. The UE determines whether the UE is located within a sub-region of an MBSFN area based on one or more parameters. The UE receives based on the determination a service over broadcast in the sub-region of the MBSFN area or over unicast outside the sub-region. In an aspect, the MBSFN area may be smaller than a unicast area. In an aspect, adaptive retransmission with a group NACK approach may be used to improve an SNR and reliability.

Abstract: A method, an apparatus, and a computer program product for wireless communication are provided. The apparatus may be a UE. The UE determines whether the UE is located within a sub-region of an MBSFN area based on one or more parameters. The UE receives based on the determination a service over broadcast in the sub-region of the MBSFN area or over unicast outside the sub-region. In an aspect, the MBSFN area may be smaller than a unicast area. In an aspect, adaptive retransmission with a group NACK approach may be used to improve an SNR and reliability.

Abstract: Methods and systems for searching genomes for potential CRISPR off-target sites are provided. In preferred embodiments, the methods include identifying possible on- and off-target cleavage sites and /or ranking the potential off-target sites based on the number and location of mismatches, insertions, and/or deletions in the gRNA guide sequence relative to the genomic DNA sequence at a putative target site in the genome. These methods allow for the selection of better target sites and/or experimental confirmation of off-target sites and are an improvement over partial search mechanisms that fail to locate every possible target site.

Abstract: Methods and systems for searching genomes for potential CRISPR off-target sites are provided. In preferred embodiments, the methods include identifying possible on- and off-target cleavage sites and/or ranking the potential off-target sites based on the number and location of mismatches, insertions, and/or deletions in the gRNA guide sequence relative to the genomic DNA sequence at a putative target site in the genome. These methods allow for the selection of better target sites and/or experimental confirmation of off-target sites and are an improvement over partial search mechanisms that fail to locate every possible target site.

Abstract: This disclosure describes a composition and method of magnetic nanoparticles (MNP) that are bound to a baculovirus (BV). The MNP-BV can be systemically administered to a patient, and a strong magnetic field applied to the target tissue, thus allowing uptake and expression only in the target tissue. Off-target effects are not seen because the MNP-BC is inactivated by the complement system outside of the magnetic field.

Abstract: This disclosure is in the area of research and therapeutics. In certain embodiments, it provides methods to assist in the purification of cell mixtures, e.g., cardiomyocytes, using molecular beacons targeting cell-type specific RNA, e.g. mRNA.

Abstract: A new signal amplification method exploiting the dense atom packing in metallic nanocrystals has been developed for detecting target substances. By dissolving nanocrystals to individual ions that are stoichiometrically converted to chromophores and quantified photometrically, extremely high signal amplification can be achieved. Signal amplification is fully determined by the total number of atoms in the nanocrystals bound to a single target molecule. The disclosed nanocrystal amplification method can be implemented with a rich selection of metal/metal oxide nanocrystals and metal-reactive chromogenic substrates.

Abstract: A new signal amplification method exploiting the dense atom packing in metallic nanocrystals has been developed for detecting target substances. By dissolving nanocrystals to individual ions that are stoichiometrically converted to chromophores and quantified photometrically, extremely high signal amplification can be achieved. Signal amplification is fully determined by the total number of atoms in the nanocrystals bound to a single target molecule. The disclosed nanocrystal amplification method can be implemented with a rich selection of metal/metal oxide nanocrystals and metal-reactive chromogenic substrates. The chromogenic reactions can be either solution-based or surface-based and performed in aqueous or organic phase, supporting a variety of assay formats.

Abstract: The present application provides materials and methods for treating hemoglobinopathies. More specifically, the application provides methods for producing progenitor cells that are genetically modified via genome editing to increase the production of fetal hemoglobin (HbF), as well as modified progenitor cells (including, for example, CD34+ human hematopoietic stem cells) producing increased levels of HbF, and methods of using such cells for treating hemoglobinopathies such as sickle cell anemia and ?-thalassemia.

Abstract: Techniques are provided for smooth transition between broadcast/multicast transmission and unicast transmission. A method for managing unicast and multicast services switching in a multi-band/multi-frequency wireless communications system (WCS) capable of both unicast and multicast signaling includes determining whether an aggregate demand for a content provided via dedicated unicast transmissions exceeds a threshold. The method includes switching transmission of the content from the dedicated unicast transmissions on one frequency to a multicast transmission on a second or more frequencies in response to determining that the aggregate demand exceeds the threshold.

Abstract: This disclosure describes a composition and method of magenitic nanoparticles (MNP) that are bound to a baculovirus (BV). The MNP-BV can be systemically administered to a patient, and a strong magnetic field applied to the target btissue, thus allowing uptake and expression only in the target tissue. Off-target effects are not seen because the MNP-BC is inactivated by the complement system outside of the magnetic field.

Abstract: Methods and systems for searching genomes for potential CRISPR off-target sites are provided. In preferred embodiments, the methods include identifying possible on- and off-target cleavage sites and/or ranking the potential off-target sites based on the number and location of mismatches, insertions, and/or deletions in the gRNA guide sequence relative to the genomic DNA sequence at a putative target site in the genome. These methods allow for the selection of better target sites and/or experimental confirmation of off-target sites and are an improvement over partial search mechanisms that fail to locate every possible target site.

Abstract: This disclosure is in the area of research and therapeutics. In certain embodiments, it provides methods to assist in the purification of cell mixtures, e.g., cardiomyocytes, using molecular beacons targeting cell-type specific RNA, e.g. mRNA.

Abstract: A method, an apparatus, and a computer program product for wireless communication are provided. The apparatus may be an MCE. The apparatus receives a plurality of channel status reports from respective eNBs. In an aspect, each channel status report includes channel information associated with a respective eNB for one of a plurality of channels in a contention based radio frequency band. The apparatus selects a channel for an eNB among the plurality of channels based on the plurality of channel status reports. In an aspect, the selected channel is available to be used for an eMBMS. The apparatus prompts the eNB to provide the eMBMS using the selected channel.