Patents by Inventor George B. Sigal

George B. Sigal has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20180154353
    Abstract: We describe assay modules (e.g., assay plates, cartridges, multi-well assay plates, reaction vessels, etc.), processes for their preparation, and method of their use for conducting assays. Reagents may be present in free form or supported on solid phases including the surfaces of compartments (e.g., chambers, channels, flow cells, wells, etc.) in the assay modules or the surface of colloids, beads, or other particulate supports. In particular, dry reagents can be incorporated into the compartments of these assay modules and reconstituted prior to their use in accordance with the assay methods. A desiccant material may be used to maintain and stabilize these reagents in a dry state.
    Type: Application
    Filed: January 29, 2018
    Publication date: June 7, 2018
    Inventors: Eli N. GLEZER, Bandele JEFFREY-COKER, Jeffery D. DEBAD, Sudeep M. KUMAR, George B. SIGAL, Gisbert SPIELES, Michael TSIONSKY, Michael WARNOCK
  • Patent number: 9878323
    Abstract: We describe assay modules (e.g., assay plates, cartridges, multi-well assay plates, reaction vessels, etc.), processes for their preparation, and method of their use for conducting assays. Reagents may be present in free form or supported on solid phases including the surfaces of compartments (e.g., chambers, channels, flow cells, wells, etc.) in the assay modules or the surface of colloids, beads, or other particulate supports. In particular, dry reagents can be incorporated into the compartments of these assay modules and reconstituted prior to their use in accordance with the assay methods. A desiccant material may be used to maintain and stabilize these reagents in a dry state.
    Type: Grant
    Filed: October 29, 2012
    Date of Patent: January 30, 2018
    Assignee: MESO SCALE TECHNOLOGIES, LLC
    Inventors: Eli N. Glezer, Bandele Jeffrey-Coker, Jeffery D. Debad, Sudeep M. Kumar, George B. Sigal, Gisbert Spieles, Michael Tsionsky, Michael Warnock
  • Publication number: 20160370359
    Abstract: A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.
    Type: Application
    Filed: September 2, 2016
    Publication date: December 22, 2016
    Inventors: George B. SIGAL, Jacob N. WOHLSTADTER, Satyanarayana GUDIBANDE, Mark T. MARTIN, James L. WILBUR
  • Patent number: 9448235
    Abstract: A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.
    Type: Grant
    Filed: November 23, 2009
    Date of Patent: September 20, 2016
    Assignee: MESO SCALE TECHNOLOGIES LLC.
    Inventors: George B. Sigal, Jacob N. Wohlstadter, Satyanarayana Gudibande, Mark T. Martin, James L. Wilbur
  • Publication number: 20140274805
    Abstract: Materials and Methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.
    Type: Application
    Filed: April 10, 2014
    Publication date: September 18, 2014
    Applicant: MESO SCALE TECHNOLOGIES LLC
    Inventors: Jacob N. WOHLSTADTER, James L. WILBUR, George B. SIGAL, Mark T. MARTIN, Liang-Hong GUO, Alan FISCHER, Jon LELAND, Mark A. BILLADEAU, Larry R. HELMS, Ramin DARVARI
  • Patent number: 8722323
    Abstract: Materials and methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.
    Type: Grant
    Filed: November 2, 2010
    Date of Patent: May 13, 2014
    Assignee: Meso Scale Technologies LLP
    Inventors: Jacob N. Wohlstadter, James L. Wilbur, George B. Sigal, Mark T. Martin, Liang-Hong Guo, Alan Fischer, Jon Leland, Mark A. Billadeau, Larry R. Helms, Ramin Darvari
  • Patent number: 8541174
    Abstract: Materials and methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.
    Type: Grant
    Filed: December 14, 2005
    Date of Patent: September 24, 2013
    Assignee: Meso Scale Technologies, LLC
    Inventors: Jacob N. Wohlstadter, James L. Wilbur, George B. Sigal, Mark T. Martin, Liang-Hong Guo, Alan Fischer, Jon Leland, Mark A. Billadeau
  • Publication number: 20110124116
    Abstract: Materials and methods are provided for producing patterned multi-array, multi-specific surfaces for use in diagnostics. The invention provides for electrochemiluminescence methods for detecting or measuring an analyte of interest. It also provides for novel electrodes for ECL assays. Materials and methods are provided for the chemical and/or physical control of conducting domains and reagent deposition for use multiply specific testing procedures.
    Type: Application
    Filed: November 2, 2010
    Publication date: May 26, 2011
    Applicant: MESO SCALE TECHNOLOGY LLP
    Inventors: Jacob N. WOHLSTADTER, James L. Wilbur, George B. Sigal, Mark T. Martin, Liang-Hong Guo, Alan Fischer, Jon Leland, Mark A. Billadeau, Larry R. Helms, Ramin Darvari
  • Publication number: 20100140086
    Abstract: A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.
    Type: Application
    Filed: November 23, 2009
    Publication date: June 10, 2010
    Applicant: MESO SCALE TECHNOLOGIES, LLC
    Inventors: George B. Sigal, Jacob N. Wohlstadter, Satyanarayana Gudibande, Mark T. Martin, James L. Wilbur
  • Patent number: 7682788
    Abstract: A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.
    Type: Grant
    Filed: July 2, 2004
    Date of Patent: March 23, 2010
    Assignee: Meso Scale Technologies, LLC
    Inventors: George B. Sigal, Jacob N. Wohlstadter, Satyanarayana Gudibande, Mark T. Martin, James L. Wilbur
  • Patent number: 6846629
    Abstract: Microparticles comprising a core and electrochemiluminescent moieties within the core and methods of using the same in assays.
    Type: Grant
    Filed: June 29, 2001
    Date of Patent: January 25, 2005
    Assignee: MesoScale Technologies, LLC.
    Inventors: George B. Sigal, Jacob N. Wohlstadter, Satyanarayana Gudibande, Mark T. Martin, James L. Wilbur
  • Publication number: 20040259148
    Abstract: A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electorchemical energy is aplied to it in the presence of said mixture.
    Type: Application
    Filed: July 2, 2004
    Publication date: December 23, 2004
    Inventors: George B. Sigal, Jacob N. Wohlstadter, Satyanarayana Gudibande, Mark T. Martin, James L. Wilbur
  • Patent number: 6809196
    Abstract: An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
    Type: Grant
    Filed: July 25, 2001
    Date of Patent: October 26, 2004
    Assignee: President and Fellows of Harvard College
    Inventors: Cynthia C. Bamdad, George B. Sigal, Jack L. Strominger, George M. Whitesides
  • Patent number: 6808939
    Abstract: Bipyridine or phenanthroline ligands presenting functional groups that prevent non-specific binding (in particular, negatively charged functional groups that are unaffected by standard conditions for conjugating biological reagents through amide bonds) are described as are luminescent metal complexes comprising these ligands. The use of luminescent ruthenium and osmium complexes comprising these ligands in electrochemiluminescence assays shows that the use of these labels can significantly reduce the amount of non-specific binding observed relative to assays carried out using reagents labeled with analogous labels that don't present functional groups that decrease non-specific binding.
    Type: Grant
    Filed: June 29, 2001
    Date of Patent: October 26, 2004
    Assignees: IGEN International, Inc., BioVeris Corporation
    Inventors: George B. Sigal, Howie Tjiong, Liwen Dong, Md. Athar Masood, Richard C. Titmas
  • Patent number: 6673542
    Abstract: A method for assaying a sample for a nucleic acid damaging activity using at least one singular double-stranded nucleic acid with at least one electrochemiluminescent label, and a method for measuring an inhibitor of a nucleic acid damaging activity with at least one singular double-stranded nucleic acid using at least one electrochemiluminescent label, are disclosed.
    Type: Grant
    Filed: March 7, 2001
    Date of Patent: January 6, 2004
    Assignee: IGEN International Inc.
    Inventors: Jeffrey A. Heroux, George B. Sigal, Reid W. von Borstel
  • Publication number: 20030027357
    Abstract: Bipyridine or phenanthroline ligands presenting functional groups that prevent non-specific binding (in particular, negatively charged functional groups that are unaffected by standard conditions for conjugating biological reagents through amide bonds) are described as are luminescent metal complexes comprising these ligands. The use of luminescent ruthenium and osmium complexes comprising these ligands in electrochemiluminescence assays shows that the use of these labels can significantly reduce the amount of non-specific binding observed relative to assays carried out using reagents labeled with analogous labels that don't present functional groups that decrease non-specific binding.
    Type: Application
    Filed: June 29, 2001
    Publication date: February 6, 2003
    Inventors: George B. Sigal, Howard Isaac Tjiong, Liwen Dong, Athar Masood, Richard C. Titmas
  • Publication number: 20030003460
    Abstract: The present invention relates to methods, reagents and compositions for conducting electrochemiluminescence binding assays which improve one or more characteristics of the assay or the instruments used to conduct the assay. The method is achieved using microparticles that include electrically conductive material. The electrically conductive material has one or more copies of an assay ligand immobilized an its outer surface and a plurality of electrochemiluminescent moieties immobilized an its outer surface. The assay ligand may be linked to the electrochemiluminescent moiety.
    Type: Application
    Filed: June 29, 2001
    Publication date: January 2, 2003
    Applicant: Meso Scale Technology LLP
    Inventors: George B. Sigal, Jacob N. Wohlstadter, Satyanarayana Gudibande, Mark T. Martin, James L. Wilbur
  • Publication number: 20020164593
    Abstract: A method for assaying a sample for a nucleic acid damaging activity using at least one singular double-stranded nucleic acid with at least one electrochemiluminescent label, and a method for measuring an inhibitor of a nucleic acid damaging activity with at least one singular double-stranded nucleic acid using at least one electrochemiluminescent label, are disclosed.
    Type: Application
    Filed: March 7, 2001
    Publication date: November 7, 2002
    Applicant: IGEN International, Inc.
    Inventors: Jeffrey A. Heroux, George B. Sigal, Reid W. von Borstel
  • Patent number: 6472148
    Abstract: An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
    Type: Grant
    Filed: January 21, 1997
    Date of Patent: October 29, 2002
    Assignee: President and Fellows of Harvard College
    Inventors: Cynthia C. Bamdad, George B. Sigal, Jack L. Strominger, George M. Whitesides
  • Publication number: 20020042074
    Abstract: An article suitable for use as a biosensor includes a molecule of a formula X—R—Ch adhered to a surface of the article as part of a self-assembled monolayer. X is a functionality that adheres to the surface, R is a spacer moiety, and Ch is a chelating agent. A metal ion can be coordinated by the chelating agent, and a polyamino acid-tagged biological binding partner of a target biological molecule coordinated to the metal ion. A method of the invention involves bringing the article into contact with a medium containing or suspected of containing the target biological molecule and allowing the biological molecule to biologically bind to the binding partner. The article is useful particularly as a surface plasmon resonance chip.
    Type: Application
    Filed: July 25, 2001
    Publication date: April 11, 2002
    Applicant: President and Fellows of Harvard College
    Inventors: Cynthia C. Bamdad, George B. Sigal, Jack L. Strominger, George M. Whitesides