Patents by Inventor George M. Church

George M. Church has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190194709
    Abstract: The present disclosure provides methods that combine RNA fluorescent in situ sequencing (FISSEQ) with other molecular detection modalities, forming an integrated panomic detection platform. In various embodiments, the present disclosure provides systems and methods to prepare a biological sample to preserve the spatial relationships of biomolecules of interest within the biological sample for FISSEQ detection.
    Type: Application
    Filed: February 28, 2019
    Publication date: June 27, 2019
    Inventors: George M. Church, Evan R. Daugharthy, Richard C. Terry
  • Patent number: 10329587
    Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: June 25, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Kevin M. Esvelt, Prashant G. Mali
  • Publication number: 20190184006
    Abstract: Described herein are in vitro-transcribed (IVT) RNA molecules comprising, a 5? cap structure, a coding region encoding an antigen polypeptide, an immunostimulatory RNA sequence, and a poly(A) tail.
    Type: Application
    Filed: October 4, 2018
    Publication date: June 20, 2019
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Ying Kai Chan, Jessica Jing-Shiuan Chiang, George M. Church
  • Publication number: 20190177731
    Abstract: Modified viral genomes are able to reduce induction of inflammatory and immune anti-viral responses. This manifests itself in reduced NF-kB activity, increased viral transduction rates, and increased expression of transgenes. Viral genomes are modified by incorporating one or more oligonucleotide sequences which are able to bind to TLR9 but not induce activation of it. The oligonucleotide sequences may be synthetic, bacterial, human, or from any other source.
    Type: Application
    Filed: June 8, 2017
    Publication date: June 13, 2019
    Applicant: Presicent and Fellows Harvard College
    Inventors: Ying Kai Chan, George M. Church
  • Publication number: 20190177718
    Abstract: The present disclosure provides a number of targeted nucleic acid FISSEQ library construction methods. Targeted FISSEQ can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to “random” or “whole-omic” detection via FISSEQ.
    Type: Application
    Filed: February 26, 2019
    Publication date: June 13, 2019
    Inventors: George M. Church, Evan R. Daugharthy, Richard C. Terry, Benjamin W. Pruitt, Brian M. Turczyk
  • Publication number: 20190177720
    Abstract: A method of processing a collection of nucleic acid sequences is provided including connecting an adaptor to one or more or each nucleic acid sequence in the collection to create a processed nucleic acid template library, wherein the adaptor includes a first DNA sequence encoding a PAM sequence and at least a tracr mate.
    Type: Application
    Filed: June 21, 2017
    Publication date: June 13, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: Reza Kalhor, Javier Fernandez Juarez, Henry Hung-yi Lee, George M. Church
  • Publication number: 20190161750
    Abstract: Methods and compositions for high-throughput, single cell analyses are provided. The methods and compositions can be used for analysis of genomes and transcriptomes, as well as antibody discovery, HLA typing, haplotyping and drug discovery.
    Type: Application
    Filed: February 8, 2019
    Publication date: May 30, 2019
    Inventors: George M. Church, Francois Vigneault
  • Publication number: 20190160118
    Abstract: A probiotic formulation is provided including one or more bacteria, bacterial strains or bacterial species of the genus Veillonella, genus Faecalibacterium, genus Phascolarctobacteria, genus Oscillospira, genus Ruminococcus, genus Bacteroides, genus Blautia, family Christensenellaceae, genus Dialister, or phylum cyanobacteria.
    Type: Application
    Filed: April 10, 2017
    Publication date: May 30, 2019
    Inventors: Jonathan Scheiman, George M. Church
  • Publication number: 20190161743
    Abstract: CRISPR/Cas9 methods are provided where a guide RNA is engineered to self-target and inactivate a nucleic acid encoding the guide RNA itself.
    Type: Application
    Filed: May 9, 2017
    Publication date: May 30, 2019
    Inventors: George M. Church, Reza Kalhor, Prashant G. Mali
  • Publication number: 20190155835
    Abstract: Methods, systems, and computer-readable media for processing spatially related sequence data received from a sequencing device are presented. In one or more embodiments, a computing platform may receive, from a sequencing device, image data associated with a sample. The computing platform may identify, based on the image data received from the sequencing device, a first sequence located at first spatial coordinates. Subsequently, the computing platform may store, in a spatially searchable database, a first data element comprising the first spatial coordinates and a first identifier corresponding to the first sequence to spatially relate the first sequence to other sequences present in the sample.
    Type: Application
    Filed: November 3, 2016
    Publication date: May 23, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: Evan R. Daugharthy, Vivek Dasari, George M. Church
  • Publication number: 20190145013
    Abstract: The present disclosure provides methods of activating an enzyme, such as error prone or template independent polymerase, using electricity to alter pH of a reaction zone and reaction site from an inactivating pH at which the enzyme is inactive to an activating pH at which the enzyme is active to add a nucleotide to an initiator or growing polymer chain. The activating pH can then be changed back to an inactivating pH and the process repeated as many times as desired to produce a target nucleic acid sequence.
    Type: Application
    Filed: May 9, 2017
    Publication date: May 16, 2019
    Inventors: Reza Kalhor, Henry Hung-yi Lee, George M. Church
  • Patent number: 10289801
    Abstract: A method of storing information using monomers such as nucleotides is provided including converting a format of information into a plurality of bit sequences of a bit stream with each having a corresponding bit barcode, converting the plurality of bit sequences to a plurality of corresponding oligonucleotide sequences using one bit per base encoding, synthesizing the plurality of corresponding oligonucleotide sequences on a substrate having a plurality of reaction locations, and storing the synthesized plurality of corresponding oligonucleotide sequences.
    Type: Grant
    Filed: August 4, 2017
    Date of Patent: May 14, 2019
    Assignee: President and Fellows of Harvard College
    Inventor: George M. Church
  • Publication number: 20190136309
    Abstract: This invention provides methods and systems of DNA synthesis including providing an encoding unit comprising an enzyme, a single-stranded DNA (ssDNA) and a nanopore, providing a lipid bilayer having on opposite sides a cis and a trans reservoir each having a different buffer composition, wherein the nanopore is within the lipid bilayer and the enzyme and the ssDNA are in the cis reservoir, providing an electrode over the lipid bilayer wherein the electrode can modulate voltage across the lipid bilayer, wherein the enzyme catalyzes DNA synthesis in response to the voltage.
    Type: Application
    Filed: April 19, 2017
    Publication date: May 9, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: George M. Church, Mirkó Palla, Peter Benjamin Stranges, Jeffrey Matthew Nivala
  • Publication number: 20190127762
    Abstract: Inhibiting p53 or Bax can be used to improve nuclease-mediated gene targeting frequencies in stem cells. This inhibition can be achieved, e.g., by overexpression of anti-apoptosis proteins or by silencing or reducing p53 or Bax expression. This technique can be used in conjunction with other rapidly developing CRISPR technologies, including improvements in specificity, other types of nucleases, and further enrichment, screening, and selection schemes, to expand the use of stem cells in experimental studies and tissue engineering for therapeutic purposes.
    Type: Application
    Filed: April 19, 2017
    Publication date: May 2, 2019
    Inventors: George M. Church, Susan M. Byrne
  • Patent number: 10273501
    Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: April 30, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Prashant G. Mali, Luhan Yang
  • Publication number: 20190121936
    Abstract: A method of storing information using monomers such as nucleotides is provided including converting a format of information into a plurality of bit sequences of a bit stream with each having a corresponding bit barcode, converting the plurality of bit sequences to a plurality of corresponding oligonucleotide sequences using one bit per base encoding, synthesizing the plurality of corresponding oligonucleotide sequences on a substrate having a plurality of reaction locations, and storing the synthesized plurality of corresponding oligonucleotide sequences.
    Type: Application
    Filed: December 12, 2018
    Publication date: April 25, 2019
    Inventor: George M. Church
  • Patent number: 10266888
    Abstract: Methods of volumetric imaging of a three-dimensional matrix of nucleic acids within a cell is provided. An automated apparatus for sequencing and volumetric imaging of a three-dimensional matrix of nucleic acids is provided.
    Type: Grant
    Filed: May 2, 2018
    Date of Patent: April 23, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Evan R. Daugharthy, Richard C. Terry, Je-Hyuk Lee, George M. Church, Benjamin W. Pruitt
  • Publication number: 20190112599
    Abstract: Methods and compositions of single tube preparation of sequencing libraries from a target DNA are provided. The methods include contacting the DNA with a composition comprising Cas9 endonuclease, a first and a second guide RNAs, a ligase, and sequencing adapters, subjecting the composition to thermal cycling to cleave the DNA at the sites flanking the regions of interest by the RNA guided endonuclease, and subjecting the composition to a temperature to allow ligation of the cleaved DNA fragments including the regions of interest with the sequencing adapters to generate the sequencing libraries.
    Type: Application
    Filed: March 29, 2017
    Publication date: April 18, 2019
    Inventors: George M. Church, Benjamin W. Pruitt, Richard C. Terry
  • Publication number: 20190112598
    Abstract: Methods and systems of cell-fee enzyme discovery and optimization are provided.
    Type: Application
    Filed: March 7, 2017
    Publication date: April 18, 2019
    Inventors: George M. CHURCH, Jameson K. ROGERS, Marc GUELL, Alexander GARRUSS
  • Publication number: 20190112626
    Abstract: The disclosure provides methods for making a polynucleotide wherein the addition of nucleotides can be physically, chemically and/or enzymatically controlled. The methods include combining a selected nucleotide, cations, an error prone or template independent DNA polymerase at a reaction site including an initiator sequence attached thereto and having a 3? terminal nucleotide, wherein the reaction reagents can be modulated and under conditions that allow covalent addition of one or more of a selected nucleotide to the 3? terminal nucleotide such that the selected nucleotide becomes a 3? terminal nucleotide, and repeating the addition step until the polynucleotide is formed.
    Type: Application
    Filed: March 30, 2017
    Publication date: April 18, 2019
    Inventors: Henry Hung-yi Lee, George M. Church, Reza Kalhor