Patents by Inventor George M. Church

George M. Church has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods of Genetic Modification of a Cell
    Publication number: 20190380314
    Abstract: The disclosure provides a method of modifying a PERV-A receptor gene in a cell. The method includes introducing into the cell a nucleic acid sequence encoding a Cas9 protein and a nucleic acid sequence encoding a guide RNA, introducing into the cell a donor nucleic acid sequence, wherein the Cas9 protein and the guide RNA are expressed and co-localize at a genomic site near or in the PERV-A receptor gene and the donor nucleic acid sequence replaces the PERV-A receptor gene by homology directed repair (HDR).
    Type: Application
    Filed: February 23, 2018
    Publication date: December 19, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: George M. Church, Ellen L. Shrock, Yinan Kan
  • Sequencing by structure assembly
    Patent number: 10501791
    Abstract: A method of sequencing nucleic acids is provided using sequencing by ligation and/or sequencing by hybridization.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: December 10, 2019
    Inventors: George M. Church, Richard C. Terry, Frederic Vigneault, Francois Vigneault
  • Mutant Cas9 Proteins
    Publication number: 20190367892
    Abstract: Methods of making mutant Cas9 proteins are described.
    Type: Application
    Filed: June 13, 2019
    Publication date: December 5, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: Alejandro Chavez, Frank J. Poelwijk, George M. Church
  • Orthogonal Cas9 Proteins for RNA-Guided Gene Regulation and Editing
    Publication number: 20190367948
    Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes.
    Type: Application
    Filed: May 14, 2019
    Publication date: December 5, 2019
    Inventors: George M. Church, Kevin M. Esvelt, Prashant G. Mali
  • Method for generating a three-dimensional nucleic acid containing matrix
    Patent number: 10494662
    Abstract: Methods of making a three-dimensional matrix of nucleic acids within a cell is provided.
    Type: Grant
    Filed: October 18, 2017
    Date of Patent: December 3, 2019
    Inventors: George M. Church, Je-Hyuk Lee, Richard C. Terry, Evan R. Daugharthy
  • Processive Template Independent DNA Polymerase Variants
    Publication number: 20190360013
    Abstract: An enzymatic method of making a polynucleotide is provided. The method includes combining a selected nucleotide triphosphate, one or more cations, a template-independent polymerase, and an associated processivity factor in an aqueous reaction medium including a target substrate comprising an initiator sequence and having a 3? terminal nucleotide attached to a single stranded portion, such that the template-independent polymerase and the associated processivity factor interact with the target substrate under conditions which covalently add one or more of the selected nucleotide triphosphate to the 3? terminal nucleotide. Also provided are mutant template-independent polymerases having a processivity factor attached thereto.
    Type: Application
    Filed: December 4, 2017
    Publication date: November 28, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: Kettner John Frederick GRISWOLD, JR., Brian M. TURCZYK, Daniel Jordan WIEGAND, George M. CHURCH, Alexander GARRUSS
  • Methods for Increasing Efficiency of Gene Editing in Cells
    Publication number: 20190352670
    Abstract: The present disclosure provides methods of improving nuclease-mediated gene targeting frequencies in cells using PFT? or bFGF.
    Type: Application
    Filed: January 31, 2018
    Publication date: November 21, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: George M. Church, Luhan Yang
  • Multiplexed Genome Editing
    Publication number: 20190350177
    Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.
    Type: Application
    Filed: August 5, 2019
    Publication date: November 21, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: George M. Church, Luhan Yang, Marc Guell
  • Gene Therapy Methods for Age-Related Diseases and Conditions
    Publication number: 20190345224
    Abstract: Methods of gene therapy are provided for treating or preventing age-related diseases or conditions by regulating one or more functional proteins associated with age-related diseases or conditions.
    Type: Application
    Filed: May 22, 2017
    Publication date: November 14, 2019
    Inventors: Noah Davidsohn, George M. Church
  • AAV Split Cas9 Genome Editing and Transcriptional Regulation
    Publication number: 20190345483
    Abstract: The invention provides methods of altering a target nucleic acid in a cell using the AAV split Cas9 platform. The methods comprise providing the cell an enzymatically active Cas9 and optionally a transcriptional regulator fused thereto and guide RNA having different spacer sequence lengths wherein the guide RNA directs the enzymatically active Cas9 and optionally a transcriptional regulator fused thereto to either cleave a target nucleic acid or regulate expression of a target nucleic acid.
    Type: Application
    Filed: May 12, 2017
    Publication date: November 14, 2019
    Inventors: George M. Church, Wei Leong Chew
  • Methods of Generating Libraries of Nucleic Acid Sequences for Detection via Fluorescent in Situ Sequ
    Publication number: 20190330617
    Abstract: The present disclosure provides a number of targeted nucleic acid FISSEQ library construction methods. Targeted FISSEQ can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to “random” or “whole-omic” detection via FISSEQ.
    Type: Application
    Filed: July 1, 2019
    Publication date: October 31, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: George M. Church, Evan R. Daugharthy, Richard C. Terry, Benjamin W. Pruitt, Brian M. Turczyk
  • Methods of storing information using nucleic acids
    Patent number: 10460220
    Abstract: The present invention relates to methods of storing data using one or more nucleic acids.
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: October 29, 2019
    Assignee: President and Fellows of Harvard College
    Inventor: George M. Church
  • Modulation of Enzymatic Polynucleotide Synthesis Using Chelated Divalent Cations
    Publication number: 20190323050
    Abstract: Methods and apparatus of modulating polynucleotide synthesis are provided. The methods include delivering reagents comprising enzymes, nucleotides and ions to oligonucleotide primers wherein the reagents catalyze incorporation of the nucleotides to 3? ends of the oligonucleotide primers, and modulating incorporation of the nucleotides to the 3? ends of the oligonucleotide primers. Polynucleotide synthesis is modulated by modulating presence or absence of catalytic cation cofactors to provide sequence defined synthesis of polynucleotides. In certain embodiments, the polynucleotides encode information.
    Type: Application
    Filed: December 21, 2017
    Publication date: October 24, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: Kettner John Frederick Griswold, Jr., Howon Lee, George M. Church
  • RNA-guided human genome engineering
    Patent number: 10435708
    Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
    Type: Grant
    Filed: February 12, 2016
    Date of Patent: October 8, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Prashant G. Mali, George M. Church, Luhan Yang
  • Mutant Cas9 proteins
    Patent number: 10435679
    Abstract: Methods of making mutant Cas9 proteins are described.
    Type: Grant
    Filed: October 11, 2018
    Date of Patent: October 8, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Alejandro Chavez, Frank J. Poelwijk, George M. Church
  • Engineered Guide RNA Sequences for In Situ Detection and Sequencing
    Publication number: 20190264202
    Abstract: A functional engineered guide RNA sequence is provided including a spacer sequence and a scaffold sequence, wherein the scaffold sequence includes a primer binding site for reverse transcription.
    Type: Application
    Filed: November 1, 2017
    Publication date: August 29, 2019
    Inventors: George M. Church, Reza Kalhor, Prashant G. Mali
  • High-throughput immune sequencing
    Patent number: 10392726
    Abstract: Methods and compositions for determining and/or monitoring the immune state of an individual are provided.
    Type: Grant
    Filed: October 11, 2011
    Date of Patent: August 27, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Francois Vigneault, Uri Laserson, Ido Bachelet
  • Methods of Storing Information Using Nucleic Acids
    Publication number: 20190258909
    Abstract: The present invention relates to methods of storing data using one or more nucleic acids.
    Type: Application
    Filed: May 3, 2018
    Publication date: August 22, 2019
    Applicant: President and Fellows of Harvard College
    Inventor: George M. Church
  • De novo design of allosteric proteins
    Patent number: 10385333
    Abstract: Methods and compositions for making and isolating allosteric DNA binding proteins that bind to one or more allosteric effectors to induce a conformation change in the proteins are provided.
    Type: Grant
    Filed: July 19, 2018
    Date of Patent: August 20, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Srivatsan Raman, Noah D. Taylor, George M. Church
  • RNA-Guided Human Genome Engineering
    Publication number: 20190249194
    Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
    Type: Application
    Filed: April 29, 2019
    Publication date: August 15, 2019
    Applicant: President and Fellows of Harvard College
    Inventors: George M. Church, Prashant G. Mali, Luhan Yang