Abstract: The present invention pertains to a method of detection, by mass spectrometry, of at least one marker of at least one mechanism of resistance to at least one antimicrobial, resistance of at least one microorganism contained in a sample, characterised in that the antimicrobial is a cephalosporin, and said resistance markers are proteins or peptides. Preferably, said proteins or peptides are proteins from said microorganism.

Abstract: Disclosed is a process for detecting and/or identifying, in a biological sample, bacteria exhibiting a resistance to carbapenems, including: a) contacting said sample with a reaction medium including at least one chromogenic agent and faropenem and/or doripenem; b) incubating the whole so as to allow the bacteria to grow; and c) detecting the strains exhibiting a resistance to carbapenems. The medium employed in step a) also contains cloxacillin and/or a combination of cloxacillin and PAbetaN.

Abstract: A process for detecting and/or identifying, in a biological sample, bacteria exhibiting a resistance to carbapenems by producing carbapenemase, includes: a) contacting said sample with a reaction medium including at least one carbapenem-class antibiotic, and cloxacillin; b) incubating the whole so as to allow the bacteria to grow; and c) detecting the strains exhibiting enzymatic resistance to carbapenems. Advantageously, the medium employed in step a) also contains phenylalanine-arginine-beta-naphthylamide (PAbetaN).

Abstract: A reaction medium for detecting and/or identifying methicillin-resistant Staphylococcus aureus (MRSA) bacteria includes a chromogenic substrate, a first antibiotic that belongs to the cephalosporin family and a second antibiotic that belongs to the aminoglycoside family.

Abstract: A reaction medium for detecting and/or identifying Methicillin-resistant Staphylococcus aureus (MRSA) bacteria, comprising a predetermined combination of two antibiotics, a first antibiotic which belongs to the cephalosporin family and a second antibiotic, said first and second antibiotics each being at a sub-inhibitory concentration.

Abstract: A method for distinguishing among a first group of microorganisms, belonging to a first taxon of yeasts that is resistant to an antifungal; a second group of microorganisms, belonging to a second taxon of yeasts that is different than the first taxon of yeasts, and that exhibits the mechanism of resistance to the antifungal exhibited by the first group; and a third group that is not resistant to the antifungal.

Abstract: A method for distinguishing among a first group of microorganisms belonging to a first taxon of Gram negative bacteria, the first group of bacteria exhibiting a mechanism of resistance to a treatment; a second group of microorganisms belonging to a second taxon of Gram negative bacteria, the second taxon of bacteria being different than said first taxon, and exhibiting a mechanism of resistance to a treatment identical to the mechanism of the first group; and a third group of Gram negative bacteria that is not resistant to the treatment.

Abstract: A method for distinguishing among a first group of microorganisms, belonging to a first taxon of bacteria that is vancomycin resistant; a second group of microorganisms, belonging to a second taxon of vancomycin resistant bacteria that is different than said first taxon; and a third group of microorganisms that is not resistant or has a natural resistance to vancomycin.

Abstract: The invention relates to a reaction medium for gram-negative bacteria having a beta-lactam antibiotic resistance mechanism, comprising: a marker for a beta-lactam antibiotic resistance mechanism, which is cefepime, an inhibitor of a resistance mechanism other than said beta-lactam antibiotic resistance mechanism.

Abstract: A reaction medium for detecting and/or identifying Methicillin-resistant Staphylococcus aureus (MRSA) bacteria, comprising a predetermined combination of two antibiotics, a first antibiotic which belongs to the cephalosporin family and a second antibiotic, said first and second antibiotics each being at a sub-inhibitory concentration.

Abstract: A reaction medium for detecting and/or identifying methicillin-resistant Staphylococcus aureus (MRSA) bacteria includes a chromogenic substrate, a first antibiotic that belongs to the cephalosporin family and a second antibiotic that belongs to the aminoglycoside family.

Abstract: The invention relates to the use of a combination of two culture media for distinguishing at least three groups of microorganisms in a biological sample, comprising: a first group of microorganisms, belonging to a first taxon of microorganisms and comprising at least a first mechanism of resistance to a first treatment; a second group of microorganisms, belonging to a second taxon of microorganisms and comprising at least a second mechanism of resistance to a second treatment; a third group of microorganisms, that are not resistant to said first and second treatments, said combination of two culture media comprising: a. at least a first substrate for detecting at least a first enzymatic or metabolic activity of said first group of microorganisms; b. at least two markers for differentiating the first group of microorganisms and the second group of microorganisms; c. at least one antimicrobial that is active on said third group of microorganisms.