Patents by Inventor Glenn Gibson
Glenn Gibson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11542529Abstract: The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5? exonuclease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.Type: GrantFiled: February 24, 2020Date of Patent: January 3, 2023Assignee: Codex DNA, Inc.Inventors: Lei Young, Hamilton O. Smith, Daniel Glenn Gibson
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Publication number: 20200190539Abstract: The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5? exonuclease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.Type: ApplicationFiled: February 24, 2020Publication date: June 18, 2020Inventors: Lei Young, Hamilton O. Smith, Daniel Glenn Gibson
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Patent number: 10577629Abstract: The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5? exonuclease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.Type: GrantFiled: December 20, 2016Date of Patent: March 3, 2020Assignee: SGI-DNA, Inc.Inventors: Lei Young, Hamilton O. Smith, Daniel Glenn Gibson
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Publication number: 20170233764Abstract: The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5? exonuclease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.Type: ApplicationFiled: December 20, 2016Publication date: August 17, 2017Inventors: Lei Young, Hamilton O. Smith, Daniel Glenn Gibson
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Patent number: 9534251Abstract: The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5? exonuclease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.Type: GrantFiled: March 30, 2010Date of Patent: January 3, 2017Assignee: Synthetic Genomics, Inc.Inventors: Lei Young, Hamilton O. Smith, Daniel Glenn Gibson
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Publication number: 20130295645Abstract: The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.Type: ApplicationFiled: April 16, 2013Publication date: November 7, 2013Inventors: Daniel Glenn Gibson, Hamilton O. Smith
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Patent number: 8435736Abstract: The present invention relates to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest.Type: GrantFiled: June 22, 2010Date of Patent: May 7, 2013Assignee: Synthetic Genomics, Inc.Inventors: Daniel Glenn Gibson, Hamilton O. Smith
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Patent number: 7883874Abstract: Novel strains of Bifidobacterium hifidum capable of producing a novel galactosidase enzyme activity that converts lactose to a novel mixture of galactooligosaccharides. The mixture of oligosaccharidcs may be incorporated into numerous food products or animal feeds for improving gut health by promoting the growth of bifidobacteria in the gut, and repressing the growth of the pathogenic microflora.Type: GrantFiled: June 30, 2004Date of Patent: February 8, 2011Assignee: Clasado Inc.Inventors: Glenn Gibson, Jacek Witold Slupinski, Georgios Tzortzis, Anthony Graham Wynne
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Publication number: 20100311126Abstract: The present invention relates, e.g., to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising (a) chewing back the DNA molecules with an enzyme having an exonuclease activity, to yield single-stranded overhanging portions of each DNA molecule which contain a sufficient length of the region of sequence identity to hybridize specifically to each other; (b) specifically annealing the single-stranded overhangs; and (c) repairing single-stranded gaps in the annealed DNA molecules and sealing the nicks thus formed (ligating the nicked DNA molecules). The region of sequence identity generally comprises at least 20 non-palindromic nucleotides (nt), e.g., at least about 40 non-palindromic nt.Type: ApplicationFiled: June 22, 2010Publication date: December 9, 2010Inventors: Daniel Glenn GIBSON, Hamilton O. SMITH
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Patent number: 7776532Abstract: The present invention relates, e.g., to an in vitro method, using isolated protein reagents, for joining two double-stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising (a) chewing back the DNA molecules with an enzyme having an exonuclease activity, to yield single-stranded overhanging portions of each DNA molecule which contain a sufficient length of the region of sequence identity to hybridize specifically to each other; (b) specifically annealing the single-stranded overhangs; and (c) repairing single-stranded gaps in the annealed DNA molecules and sealing the nicks thus formed (ligating the nicked DNA molecules). The region of sequence identity generally comprises at least 20 non-palindromic nucleotides (nt), e.g., at least about 40 non-palindromic nt.Type: GrantFiled: August 11, 2006Date of Patent: August 17, 2010Assignee: Synthetic Genomics, Inc.Inventors: Daniel Glenn Gibson, Hamilton O. Smith
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Publication number: 20100184187Abstract: The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5? exonuclease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.Type: ApplicationFiled: March 30, 2010Publication date: July 22, 2010Inventors: Lei YOUNG, Hamilton O. Smith, Daniel Glenn Gibson
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Patent number: 7723077Abstract: The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5? exonculease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.Type: GrantFiled: August 11, 2006Date of Patent: May 25, 2010Assignee: Synthetic Genomics, Inc.Inventors: Lei Young, Hamilton O. Smith, Daniel Glenn Gibson
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Publication number: 20090022849Abstract: The invention concerns the prebiotic effects of dark fruit and their juices such as blackcurrant, cranberry and pomegranate juices. The invention is also directed to the use of the a dark fruit or dark fruit juice for manufacture of an orally ingestable composition to promote health by promoting the growth of beneficial gut microflora. The beverage can be made from one or more dark fruit juices, in combination with other prebiotics and various other ingredients.Type: ApplicationFiled: March 9, 2005Publication date: January 22, 2009Inventors: Mike Clifford, Glenn Gibson, Henglong Hu, Andrea Rodig-Penman
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Publication number: 20070274955Abstract: Novel strains of Bifidobacterium hifidum capable of producing a novel galactosidase enzyme activity that converts lactose to a novel mixture of galactooligosaccharides. The mixture of oligosaccharidcs may be incorporated into numerous food products or animal feeds for improving gut health by promoting the growth of bifidobacteria in the gut, and repressing the growth of the pathogenic microflora.Type: ApplicationFiled: June 30, 2004Publication date: November 29, 2007Inventors: Glenn Gibson, Jacek Slupinski, Georgios Tzortzis, Anthony Wynne
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Publication number: 20070196890Abstract: A method for evaluating or quantifying the prebiotic capability of a fiber or for identifying a prebiotic substance, which comprises (a) a step of evaluating or quantifying the effect by the tested fiber or substance on the growth and/or modification of faecal bacterial population, and (b) a step of quantifying at least one product resulting from the fermentation of the tested fiber or substance and/or a step of quantifying the rate of assimilation of the tested fiber or substance.Type: ApplicationFiled: October 1, 2004Publication date: August 23, 2007Inventors: Jelena Vulevic, Glenn Gibson, Robert Rastall
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Publication number: 20060286258Abstract: The present invention is directed to a novel method for increasing the production of acetate, decreasing the production of butyrate, increasing the population and species of beneficial bacteria and slowing the rate of fermentation of prebiotics within the gut of a formula-fed infant. The method comprises administration of a therapeutically effective amount of PDX to the infant.Type: ApplicationFiled: June 30, 2005Publication date: December 21, 2006Inventors: Bryon Petschow, Robert McMahon, Glenn Gibson, Robert Rastall, Renia Gemmell, Maria Saarela, Anna-Marja Aura
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Publication number: 20060165670Abstract: The invention concerns a composition comprising a Lactobacillus strain and a non-digestible oligosaccaride.Type: ApplicationFiled: April 7, 2004Publication date: July 27, 2006Inventors: Michael Beer, Glenn Gibson, Christopher Smejkal
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Patent number: 6936598Abstract: A method for reducing the quantity of Desulfovibrio and/or Helicobacter spp. in the GI tract of a companion pet which comprises orally administering to the said pet a Desulfovibrio and/or Helicobacter spp. reducing quantity of a fiber or other component.Type: GrantFiled: November 21, 2003Date of Patent: August 30, 2005Assignee: Hill's Pet Nutrition, Inc.Inventors: Christina Khoo, Kathy Lynn Gross, Glenn Gibson
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Publication number: 20050113334Abstract: A method for reducing the quantity of Desulfovibrio and/or Helicobacter ssp. in the GI tract of a companion pet which comprises orally administering to the said pet a Desulfovibrio and/or Helicobacter ssp. reducing quantity of a fiber or other component.Type: ApplicationFiled: November 21, 2003Publication date: May 26, 2005Inventors: Christina Khoo, Kathy Gross, Glenn Gibson
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Publication number: 20050031601Abstract: A novel probiotic strain termed Lactobacillus pentosus NCIMB 41114 is capable of preventing growth of pathogenic microorganisms in the GI tract. In particular, this bacterium inhibits Candida overgrowth, and can be employed to prevent and treat associated diseases, including IBS and thrush.Type: ApplicationFiled: October 11, 2002Publication date: February 10, 2005Inventors: Anthony Wynne, Glenn Gibson, Jonathan Brostoff