Patents by Inventor Gottfried Brem

Gottfried Brem has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10221411
    Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: March 5, 2019
    Assignee: NEXTTEC GMBH
    Inventors: Hamlet Balayan, Robert-Matthias Leiser, Lutz Plobner, Leonti E. Tkachenko, Gottfried Brem
  • Publication number: 20170152501
    Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.
    Type: Application
    Filed: February 10, 2017
    Publication date: June 1, 2017
    Applicant: NExtTec GmbH
    Inventors: Hamlet Balayan, Robert-Matthias Leiser, Lutz Plobner, Leonti E. Tkachenko, Gottfried Brem
  • Publication number: 20160097048
    Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.
    Type: Application
    Filed: December 11, 2015
    Publication date: April 7, 2016
    Inventors: Hamlet Balayan, Robert-Matthias Leiser, Lutz Plobner, Leonti E. Tkachenko, Gottfried Brem
  • Publication number: 20140094596
    Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.
    Type: Application
    Filed: September 20, 2013
    Publication date: April 3, 2014
    Applicant: NExtTec GmbH
    Inventors: Hamlet Balayan, Robert-Matthias Leiser, Lutz Plobner, Leonti E. Tkachenko, Gottfried Brem
  • Patent number: 8030537
    Abstract: The present invention relates to a method for recombinant manufacturing of substances, wherein cells are transformed suing a nucleotide sequence coding for the substance, the transformed cells are subjected to a cloning process, and the cells obtained in this fashion are introduced into a host organism. The present invention relates in particular to the use of the method in the production of recombinant proteins, cells and tissues. According to a further aspect, the invention relates to a method, wherein the cells of an individual are isolated, said sells are introduced into an immunoincompetent animal for further growth and the cells, tissue and/or organs cultivated in the animal are again isolated and introduced into an individual.
    Type: Grant
    Filed: July 27, 2000
    Date of Patent: October 4, 2011
    Assignee: Apogene GmbH & Co. KG
    Inventor: Gottfried Brem
  • Publication number: 20110186519
    Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.
    Type: Application
    Filed: July 6, 2010
    Publication date: August 4, 2011
    Applicant: NEx Tec GMBH
    Inventors: Hamlet Balayan, Robert-Matthias Leiser, Lutz Plobner, Leonti E. Tkachenko, Gottfried Brem
  • Patent number: 7772152
    Abstract: A composite polymer-coated sorbent with a bidisperse or oligodisperse distribution of pore sizes and having an at least partial coating on its surface, which coating comprises essentially polyanilines or derivatives of polyanilines and use thereof for the simultaneous separation and desalting of bio-macromolecules.
    Type: Grant
    Filed: November 10, 2004
    Date of Patent: August 10, 2010
    Assignee: NextTec GmbH
    Inventors: Robert-Matthias Leiser, Dimitri Valerjewich Kapoustine, Vitali Pavlovich Zubov, Hamlet Balayan, Lutz Plobner, Gottfried Brem
  • Publication number: 20080154029
    Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.
    Type: Application
    Filed: April 1, 2005
    Publication date: June 26, 2008
    Applicant: NEXTEC GMBH
    Inventors: Hamlet Balayan, Robert-Matthias Leiser, Lutz Plobner, Leonti E. Tkachenko, Gottfried Brem
  • Patent number: 7368235
    Abstract: The invention relates to a simple and rapid method for identifying mutations in a gene of interest indicating an alteration in the reading frame of the proteins derived therefrom. The inventive method more particularly relates to the use of said method for scanning the animal population.
    Type: Grant
    Filed: July 10, 2002
    Date of Patent: May 6, 2008
    Assignee: Apogene Biotechnologie GmbH & Co. KG
    Inventors: Gottfried Brem, Thomas Czerny
  • Patent number: 7351876
    Abstract: This invention relates to a method for breeding animals via cloning and the animals obtainable by the method, in particular a method for reproducing animal embryos via efficient nuclear transfer with primordial gametes.
    Type: Grant
    Filed: September 12, 2002
    Date of Patent: April 1, 2008
    Assignee: Agrobiogen GmbH Biotechnologie
    Inventors: Gottfried Brem, Gabriela Durcova-Hills, Sigrid Müller, Wolfgang Schernthaner, Hendrik Wenigerkind, Eckhard Wolf, Valeri Zakhartchenko
  • Publication number: 20080034626
    Abstract: The present invention relates to a device and a method for marking an individual and concurrently collecting a sample therefrom. Once attached to the individual, the device cannot be removed without leaving substantial signs of manipulation, so that that the association of the device to a given individual may not be altered without being noticed.
    Type: Application
    Filed: February 25, 2004
    Publication date: February 14, 2008
    Inventors: Gottfried Brem, Alexander Berner
  • Publication number: 20070293394
    Abstract: A composite polymer-coated sorbent with a bidisperse or oligodisperse distribution of pore sizes and having an at least partial coating on its surface, which coating comprises essentially polyanilines or derivatives of polyanilines and use thereof for the simultaneous separation and desalting of bio-macromolecules.
    Type: Application
    Filed: November 10, 2004
    Publication date: December 20, 2007
    Inventors: Robert-Matthias Leiser, Dimitri Kapoustine, Vitali Zubov, Hamlet Balayan, Lutz Plobner, Gottfried Brem
  • Publication number: 20070082354
    Abstract: A method for isolating RNA from RNA containing samples wherein the RNA containing sample is treated with at least one DNase and/or another enzyme like proteases or collagenases for purification of RNA in presence of a complex of ribonucleosides and the oxovanadyl ion which is capable of inhibiting RNases present in the RNA containing samples and removing the complex of ribonucleosides and the oxovanadyl ion prior to down-stream processing of the RNA of the RNA containing sample by contacting the sample of the forgoing step with a chelating agent immobilized to a support, the chelating agent having sufficient affinity to the complex of ribonucleosides and the oxovanadyl ion so that it binds to the chemical entity and the RNA containing sample is freed from the inhibitor.
    Type: Application
    Filed: September 26, 2006
    Publication date: April 12, 2007
    Applicant: NExtTec GmbH
    Inventors: Robert-Matthias Leiser, Hamlet Balayan, Nina Fritzemeier, Gottfried Brem
  • Patent number: 7198629
    Abstract: The invention relates to modified ear tags comprising a mandrel-type plate (10), a counter plate (11), in addition to a container for receiving a sample (1) and a means for obtaining the sample (4). The means for obtaining the sample (4) has a detachable hollow tip and the counter-plate (11) has no through opening for the mandrel. The invention relates more particularly to the use of the ear tags for genotypical analysis of animal populations and to a method for analyzing animal populations with the ear tags.
    Type: Grant
    Filed: December 18, 2001
    Date of Patent: April 3, 2007
    Inventor: Gottfried Brem
  • Publication number: 20060243658
    Abstract: The present invention relates to a sorbent material having a solid support substantially modified with a fluorinated polymer coating which is covalently attached to the support and the fluorinated polymer coating is containing at least one functional group, methods of obtaining the sorbent material, the use of these materials for separation of substances, a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, a device comprising the sorbent material of the invention in loose form as well as a miniaturized device comprising the sorbent material.
    Type: Application
    Filed: November 10, 2003
    Publication date: November 2, 2006
    Inventors: Vitali Zubov, Lutz Plobner, Dimitri Kapoustine, Hamlet Balayan, Makhmud Muydinov, Gottfried Brem, Robert-Matthias Leiser
  • Publication number: 20050019750
    Abstract: The invention concerns a device for isolating RNA samples from animals. This device has a sample collecting container and sampling means with means for protection against RNA-destroying enzymes. The invention concerns in particular a method for isolating RNA in biological samples which enables the search for RNA viruses in new-born babies.
    Type: Application
    Filed: October 7, 2002
    Publication date: January 27, 2005
    Inventor: Gottfried Brem
  • Publication number: 20040234987
    Abstract: The invention relates to a simple and rapid method for identifying mutations in a gene of interest indicating an alteration in the reading frame of the proteins derived therefrom. The inventive method more particularly relates to the use of said method for scanning the animal population.
    Type: Application
    Filed: July 6, 2004
    Publication date: November 25, 2004
    Inventors: Gottfried Brem, Thomas Czerny
  • Publication number: 20040116940
    Abstract: The invention relates to a device for marking animals and simultaneously taking samples. The present invention especially relates to a device by which means such steps can be simultaneously carried out, using conventional earmarks.
    Type: Application
    Filed: August 19, 2003
    Publication date: June 17, 2004
    Inventor: Gottfried Brem
  • Publication number: 20040093775
    Abstract: The invention relates to modified ear marks, comprising a mandrel-type plate (10), a counter-plate (11), in addition to a container for receiving a sample (1) and a means for obtaining said sample (4). The means for obtaining said sample (4) has a detachable hollow tip and the counter-plate (11) has no through opening for the mandrel. The invention relates more particularly to the use of said earmarks for genotypical analysis of animal populations and to a method for analysing animal population with said earmarks.
    Type: Application
    Filed: October 30, 2003
    Publication date: May 20, 2004
    Inventor: Gottfried Brem
  • Publication number: 20040072199
    Abstract: The invention relates to a method for marking samples containing DNA. At least one oligonucleotide marker is associated with the sample, and the sample is then analysed together with the oligonucleotide marker. Said oligonucleotide marker is selected from the group consisting of artificial microsatellite oligonucleotides or artificial oligonucleotides of single nucleotide polymorphisms.
    Type: Application
    Filed: October 24, 2003
    Publication date: April 15, 2004
    Inventor: Gottfried Brem