Abstract: This invention provides a method of using a porcine stem cell factor (PSCF), which may also be termed porcine “steel” factor, which is preferably membrane-bound PSCF on transfected murine STO feeder cells, for establishing pluripotent or totipotent porcine cell lines, especially for the culture of porcine primordial germ cells (PGCs).

Abstract: This invention relates to a process for breeding animals through cloning as well as animals obtainable with the process, in particular to a process for the reproduction of animal embryos via an efficient nucleus transfer with foetal fibroblasts.

Abstract: The present invention provides a method of quantification of nucleic acid sequences with minimal alterations conducting a real time PCR quantification assay with allele-specific primers. Further, the present invention provides the method for quantifying allelic differences of bovine mitochondrial DNA sequences and allele-specific primers to be used in said method.

Abstract: The present invention relates to the production of genotype arrays of a population on DNA matrices. The invention particularly relates to the use of such DNA matrices for determining features, certain genes, alleles, mutations, expression patterns, etc. in the individuals of each examined population.

Abstract: This invention relates to a method for breeding animals via cloning and the animals obtainable by the method, in particular a method for reproducing animal embryos via efficient nuclear transfer with primordial gametes.

Abstract: The present invention provides a method of quantification of nucleic acid sequences with minimal alterations conducting a real time PCR quantification assay with allele-specific primers. Further, the present invention provides the method for quantifying allelic differences of bovine mitochondrial DNA sequences and allele-specific primers to be used in said method.

Abstract: The present invention refers to a device and a procedure for the collection and initial preparation of tissue/blood or other sample of nucleated or DNA-containing cells or cell components for molecular genetic investigation. The invented device for the collection and initial preparation of samples of DNA-containing cells includes a sample receiving container and means for the collection of the sample, which is introduced into the sample receiving container after collection of the sample and seals this tightly. The sample receiving container has a base and side walls, is closed with a easily penetrable lid and has—in an area of the side walls of the container removed from the base—means to secure the introduced sample collection tool; in the container are substances to protect from DNA-degrading enzymes.

Abstract: The present invention refers to a device and a procedure for the collection and initial preparation of tissue/blood or other sample of nucleated or DNA-containing cells or cell components for molecular genetic investigation. The invented device for the collection and initial preparation of samples of DNA-containing cells includes a sample receiving container and means for the collection of the sample, which is introduced into the sample receiving container after collection of the sample and seals this tightly. The sample receiving container has a base and side walls, is closed with a easily penetrable lid and has—in an area of the side walls of the container removed from the base—means to secure the introduced sample collection tool; in the container are substances to protect from DNA-degrading enzymes.

Abstract: Process for the production of proteins with antibody activity in which one or several DNA sequences coding for this protein are introduced into the male pronucleus of a fertilized ovum of a pig or rabbit by microinjection, the ova are implanted in the oviduct of a pig or rabbit, the offspring are bred and the protein with antibody activity is isolated from their serum in the usual way whereby the DNA sequences used for the microinjection are free of bacterial foreign sequences, and are preferably used with immunoglobulin promoter and enhancer sequences.