Abstract: The invention is directed to methods for purifying Troponin I, particularly recombinant Tropnin I produced in a bacterial expression system. Recombinant Tropnin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g., by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrafhionate yielded sulfitolyzed Tropnin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.

Abstract: A motor-driven and controlled packaging machine for forming pouches from a web of material. The controls allow the machine to be run with either a continuously or intermittently advancing web. Seal bars are mounted on moveable carriages which move with the web as they form the seals. A web unwind is servo controlled to minimize the amount of festoon section needed when operating in intermittent mode. Each registration-related component of the machine has a dedicated sensor for independently registering each component with the web. Accordingly, the machine may regain registration with minimal web waste and can be quickly and easily repositioned for different pouch sizes.

Abstract: The invention is directed to methods for purifying Troponin I, particularly recombinant Tropnin I produced in a bacterial expression system. Recombinant Tropnin I can be advantageously purified after reversibly protecting the free sulfhydryl groups, e.g., by forming sulfates. In a specific example, Tropnin I reacted with sodium tetrafhionate yielded sulfitolyzed Tropnin I, which was purified by chromatography on an anion exchanger, followed by hydrophobic interaction chromatography. Facile deprotection of the sulfhydryl groups yields a highly purified product ready for refolding.

Abstract: A packaging machine for forming pouches from a web of material having web tension control. A second pair of infeed rolls allows web tension through a sealing section to be controlled. An unwind reel is power-driven according to downstream web demand to thereby minimize tension spikes through the infeed section. The power unwind further minimizes the amount of festoon area needed. An entire packaging machine may be provided using modules. The registration-related operations of each section are independently controlled so that each module need only coordinate input and/or output speeds with interfacing sections.

Abstract: A motor-driven and controlled packaging machine for forming pouches from a web of material. The controls allow the machine to be run with either a continuously or intermittently advancing web. Seal bars are mounted on moveable carriages which move with the web as they form the seals. A web unwind is servo controlled to minimize the amount of festoon section needed when operating in intermittent mode. Each registration-related component of the machine has a dedicated sensor for independently registering each component with the web. Accordingly, the machine may regain registration with minimal web waste and can be quickly and easily repositioned for different pouch sizes.

Abstract: A packaging machine for forming pouches from a web of material having web tension control. A second pair of infeed rolls allows web tension through a sealing section to be controlled. An unwind reel is power-driven according to downstream web demand to thereby minimize tension spikes through the infeed section. The power unwind further minimizes the amount of festoon area needed. An entire packaging machine may be provided using modules. The registration-related operations of each section are independently controlled so that each module need only coordinate input and/or output speeds with interfacing sections.

Abstract: A motor-driven and controlled packaging machine for forming pouches from a web of material. The controls allow the machine to be run with either a continuously or intermittently advancing web. Seal bars are mounted on moveable carriages which move with the web as they form the seals. A web unwind is servo controlled to minimize the amount of festoon section needed when operating in intermittent mode. Each registration-related component of the machine has a dedicated sensor for independently registering each component with the web. Accordingly, the machine may regain registration with minimal web waste and can be quickly and easily repositioned for different pouch sizes.

Abstract: A packaging machine for forming pouches from a web of material having web tension control. A second pair of infeed rolls allows web tension through a sealing section to be controlled. An unwind reel is power-driven according to downstream web demand to thereby minimize tension spikes through the infeed section. The power unwind further minimizes the amount of festoon area needed. An entire packaging machine may be provided using modules. The registration-related operations of each section are independently controlled so that each module need only coordinate input and/or output speeds with interfacing sections.

Abstract: A sealing mechanism for a form, fill and seal packing machine having box motion sealers suitable for forming seals in both continuously and intermittently advancing webs. The sealers are disposed on opposite sides of the web and are operable to reciprocate into and out of engagement with the web. The sealers are further supported on carriages moveable longitudinally along the web path. As a result, the sealers may be operated in the box motion in which the sealers actuate into engagement with the web as the carriages move longitudinally downstream at the web speed. The sealers remain engaged with the web for a time sufficient to form a seal in the web before retracting out of engagement. The carriages then return longitudinally upstream to initial positions. The box motion may be repeated to form a series of seals in the web.

Abstract: A packaging machine for forming pouches from a web of material having web tension control. A second pair of infeed rolls allows web tension through a sealing section to be controlled. An unwind reel is power-driven according to downstream web demand to thereby minimize tension spikes through the infeed section. The power unwind further minimizes the amount of festoon area needed. An entire packaging machine may be provided using modules. The registration-related operations of each section are independently controlled so that each module need only coordinate input and/or output speeds with interfacing sections.

Abstract: A packaging machine capable of forming pouches from both continuously and intermittently advancing webs. The machine has a pair of drive rolls adapted to advance the web that are operable both continuously and intermittently. Web-engaging components of the machine are operable to engage the web and perform their associated functions as the web is in motion. The web-engaging components are further selectively operable to have an adjustable dwell period between operations, thereby allowing the machine to be switched between continuous and intermittent modes, and to adapt the machine to form pouches of various sizes. The formed pouches are transferred to a pouch filling section which is also operable to receive and advance individual pouches either continuously or intermittently.

Abstract: The present invention provides for a method of promoting motor neuron survival, growth or differentiation comprising treating motor neurons with an effective amount of HGF. The invention also provides for a method of promoting motor neuron survival, growth or differentiation comprising treating motor neurons with an effective amount of HGF and an effective amount of CNTF. The invention further provides for a method of alleviating the toxic effects of vincristine on motor neurons comprising treating motor neurons with an effective amount of HGF and an effective amount of CNTF. The methods may be carried out in vitro or in vivo. The invention also provides a method of treating a motor neuron disorder in a patient comprising administering to the patient an effective amount of HGF and CNTF. The invention further provides a pharmaceutical composition for treating a motor neuron disorder in a patient comprising HGF and CNTF in a pharmacologically acceptable carrier.

Abstract: Pouch filling apparatus for carrying pouches through a pouch filler. The apparatus has leading and trailing clamps which are mounted on independent endless carriers. The endless carriers are driven at the same speed, but are controlled to maintain a set lag distance between the leading and trailing clamps. The lag distance may be adjusted to thereby adapt the apparatus for pouches of various sizes. The apparatus further incorporates a single retard arm and a single advance arm for moving the leading clamp between opened and closed pouch positions, respectively. The advance arm is located upstream of the pouch filler to move the leading clamp into the open pouch position for filling. The retard arm is located downstream of the pouch filler and moves the trailing clamp to the closed pouch position for sealing. The retard and advance arms are independently servo controlled to adapt the apparatus for pouches of various sizes and for continuous and intermittent operation.

Abstract: An apparatus and related method for feeding/turning successive cartons along the flow path of a packaging line is provided. An intermediate conveyor section between in-line feed and take away conveyors defines the substantially horizontal flow path. A plurality of elongated O-ring belts feed and turn the cartons. A separate side running chain having a straight operative run carries a plurality of extensible fingers; one selected finger engaging and capturing a leading corner of each successive carton to assist in turning. The O-ring belts are driven at differential speed. The combined feeding and turning action of the carton, assisted by retarding engagement of the carton corner by the finger assures positive and efficient feeding/turning of the cartons. A microprocessor is provided to control the driving of stepper/servo motors for the various components. The O-ring belts are driven by the single servo motor with a plurality of increasing size pulleys on a common shaft to provide the progressive speeds.