Abstract: The disclosure provides a cell culture apparatus having multiple stacked cell culture units each having multiple cell culture chambers. The multiple cell culture units are connected by a manifold which connects the cell culture units and provides a mechanism for efficiently filling the multiple cell culture chambers.

Abstract: A multilayered cell culture apparatus for the co-culturing of at least two cell types is disclosed. The cell co-culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between some cell culture chambers and the external environment. In addition, some cell culture chambers may have limited access to air creating relatively lower oxygen content cell culture chambers. The size of the apparatus, and location of an optional neck section, allows for its manipulation by standard automated assay equipment, further making the apparatus amenable to high throughput applications.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: A cell culture apparatus includes a cell culture chamber formed by a first major surface, an opposing second major surface spaced apart from the first major surface, and a first side wall around the first chamber and extending between the first and second major surfaces. A portion of the first sidewall proximate the first major surface comprises an infrared absorbent material, and a portion the first sidewall proximate the second major surface is formed from substantially non-infrared absorbent material. The first major surface is formed by a gas permeable polymeric film that is impermeable to cell culture liquid.

Abstract: A device for trapping extraneous gas or bubbles is disclosed. The device is defined as having a gas collection area in a tapered neck portion such that when used as a cell culturing apparatus, extraneous gas or bubbles are trapped and isolated from the cell growth areas where cells or media reside.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus includes a unitary flask body including a rigid upper and lower surface, connected by side walls. The cell growth apparatus comprises multiple cell growth chambers stacked in vertical alignment and orientation within the unitary flask body. The stacked chambers are held in position by unitary connecting columns that run through each cell growth chamber and terminate at the rigid upper and lower surfaces of the apparatus. The cell growth chambers are separated by tracheal spaces that allow air from the external environment to contact the cell growth surface of each individual cell growth chamber.

Abstract: A nested permeable support device is described herein that can be used to perform various experiments to test new therapeutic compounds (new chemical entities). In one application, the nested permeable support device is used to perform a first pass assay to determine the bioavailability of a new chemical entity following absorption through the digestive tract and metabolism by the liver.

Abstract: A device for minimizing the formation of bubbles or foam in cell culture is disclosed. The device has a manifold which directs the inflow of cells and cell culture media into a cell culture vessel so as to allow for displaced air or gas to vent from the cell culture vessel without mixing with the incoming cell culture media, thereby preventing the mixing of air and cell culture media and minimizing the formation of bubbles or foam inside the cell culture vessel.

Abstract: A device for trapping extraneous gas or bubbles is disclosed. The device is defined as having a gas collection area in a tapered neck portion such that when used as a cell culturing apparatus, extraneous gas or bubbles are trapped and isolated from the cell growth areas where cells or media reside.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: A cell culture apparatus includes cell culture units having cell culture chambers, each with at least one manifold. The manifold connects the cell culture unit to a fluid flow channel. The manifold has a dam which allows liquid to pool in the manifold and allows for the creation of an airspace in the manifold, which reduces hydrostatic pressure inside the apparatus and enables the stacking of multiple cell culture units. Embodiments include cascading manifolds, manifolds having reservoirs, and manifolds having valves.

Abstract: A wireless communication method and protocol, and wireless devices and systems for stimulation, are provided for communication between a wireless device and a charging device. During active wireless charging, communications (data transmission) from the wireless device to the charging device occurs via pulse loading the receive antenna of the receiving device. Because switching regulation in the receiving device may interfere with the communications, the switching regulation is disabled during a communications window. To further reduce the likelihood of misinterpretation of signals detected in the charging device resulting from the switching regulation or noise, the data bit rate of the pulse loading communications is maintained higher than the switching regulation frequency.

Abstract: A nested permeable support device is described herein that can be used to perform various experiments to test new therapeutic compounds (new chemical entities). In one application, the nested permeable support device is used to perform a first pass assay to determine the bioavailability of a new chemical entity following absorption through the digestive tract and metabolism by the liver.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body. also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: A method of manufacture and assembly of multiwell plates employing targeted radiation at an interface in order to achieve bonding is disclosed. The method accommodates glass and polymer attachment as well as polymer to polymer attachment. Resultant plates have unique flatness and optical properties.

Abstract: A device for trapping extraneous gas or bubbles is disclosed. The device is defined as having a gas collection area in a tapered neck portion such that when used as a cell culturing apparatus, extraneous gas or bubbles are trapped and isolated from the cell growth areas where cells or media reside.

Abstract: A method of manufacture and assembly of multiwell plates employing targeted radiation at an interface in order to achieve bonding is disclosed. The method accommodates glass and polymer attachment as well as polymer to polymer attachment. Resultant plates have unique flatness and optical properties.

Abstract: Cell culture apparatuses and methods for culturing cells include bags for culturing cells. The bags are folded and unfolded to control cell culture conditions. As the bags are folded, segmented sealed chambers for culturing cells are formed. As the bags are unfolded, the segments are unsealed allowing for exchange of fluid between the regions of the subchambers.