Abstract: Cell culture apparatuses and methods for culturing cells include bags for culturing cells. The bags are folded and unfolded to control cell culture conditions. As the bags are folded, segmented sealed chambers for culturing cells are formed. As the bags are unfolded, the segments are unsealed allowing for exchange of fluid between the regions of the subchambers.

Abstract: A cell culture apparatus includes a cell culture chamber formed by a first major surface, an opposing second major surface spaced apart from the first major surface, and a first side wall around the first chamber and extending between the first and second major surfaces. A portion of the first sidewall proximate the first major surface comprises an infrared absorbent material, and a portion the first sidewall proximate the second major surface is formed from substantially non-infrared absorbent material. The first major surface is formed by a gas permeable polymeric film that is impermeable to cell culture liquid.

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus includes a unitary flask body including a rigid upper and lower surface, connected by side walls. The cell growth apparatus comprises multiple cell growth chambers stacked in vertical alignment and orientation within the unitary flask body. The stacked chambers are held in position by unitary connecting columns that run through each cell growth chamber and terminate at the rigid upper and lower surfaces of the apparatus. The cell growth chambers are separated by tracheal spaces that allow air from the external environment to contact the cell growth surface of each individual cell growth chamber.

Abstract: The present invention provides a multi-layer cell culture device having a rectangular footprint and having multiple cell culture chambers separated by tracheal air spaces, each cell culture chamber having a port and a port cover or an external manifold structured and arranged to allow for transfer of fluid into and out of the cell culture device with reduced risk of contamination, and methods of using the device.

Abstract: A multilayered cell culture apparatus for the co-culturing of at least two cell types is disclosed. The cell co-culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between some cell culture chambers and the external environment. In addition, some cell culture chambers may have limited access to air creating relatively lower oxygen content cell culture chambers. The size of the apparatus, and location of an optional neck section, allows for its manipulation by standard automated assay equipment, further making the apparatus amenable to high throughput applications.

Abstract: A device for minimizing the formation of bubbles or foam in cell culture is disclosed. The device has a manifold which directs the inflow of cells and cell culture media into a cell culture vessel so as to allow for displaced air or gas to vent from the cell culture vessel without mixing with the incoming cell culture media, thereby preventing the mixing of air and cell culture media and minimizing the formation of bubbles or foam inside the cell culture vessel.

Abstract: A wireless communication method and protocol, and wireless devices and systems for stimulation, are provided for communication between a wireless device and a charging device. During active wireless charging, communications (data transmission) from the wireless device to the charging device occurs via pulse loading the receive antenna of the receiving device. Because switching regulation in the receiving device may interfere with the communications, the switching regulation is disabled during a communications window. To further reduce the likelihood of misinterpretation of signals detected in the charging device resulting from the switching regulation or noise, the data bit rate of the pulse loading communications is maintained higher than the switching regulation frequency.

Abstract: A cardiac stimulator for measuring pacing impedance includes a tank capacitor for delivering charge to the heart via leads, a shunt resistor, and high-impedance buffers for measuring pacing current through the shunt resistor. During the stimulation pulse, the voltage across the shunt resistor, as sampled by a high-impedance buffer, variously indicates lead and cardiac tissue resistance or capacitance. A high-impedance buffer measures the voltage between the tank capacitor and ground immediately following the stimulation pulse to allow estimation of the lead/heart tissue capacitance. A lead/heart tissue capacitance estimate is determined by look-up table is in memory or successive approximation. When the lead/heart tissue capacitance and lead resistance have been determined, a plurality of parameters for analyzing and optimizing a cardiac stimulation system may be calculated, such as the instantaneous current, the average current, the charge, and the energy delivered to the cardiac tissue.

Abstract: A device and method for detecting a signal from an analytical sample is provided. The device comprises a platen with a first and second surface, a number of collection structures extending from the second surface. Each collection structure has a capillary channel running along a common axis as the collection structure from the platen to a terminal end of the structure. Within the capillary channel are sample analytes of interest. On the first surface is disposed a number of optical elements, corresponding to each collection structure, each centered on a capillary channel. Each collection structure has a geometry that can concentrate an optical emission from within the capillary channel, along the entire length of the capillary channel, towards the first surface and optical elements, to a detection device, by means of either mirrored surface reflection or total internal reflection. The collection structure may also direct a light beam into the capillary channel.

Abstract: A cardiac stimulator for measuring pacing impedance includes a tank capacitor for delivering charge to the heart via leads, a shunt resistor, and high-impedance buffers for measuring pacing current through the shunt resistor. During the stimulation pulse, the voltage across the shunt resistor, as sampled by a high-impedance buffer, variously indicates lead and cardiac tissue resistance or capacitance. A high-impedance buffer measures the voltage between the tank capacitor and ground immediately following the stimulation pulse to allow estimation of the lead/heart tissue capacitance. A lead/heart tissue capacitance estimate is determined by look-up table is in memory or successive approximation. When the lead/heart tissue capacitance and lead resistance have been determined, a plurality of parameters for analyzing and optimizing a cardiac stimulation system may be calculated, such as the instantaneous current, the average current, the charge, and the energy delivered to the cardiac tissue.

Abstract: A method is described herein for making a multiwell plate that is used for assaying samples and is made from a plastic upper plate which forms the sidewalls of one or more wells and a glass lower plate which forms the bottom walls of the wells. The plastic upper plate and glass lower plate are attached and bound to one another by an enhanced adhesive. The enhanced adhesive includes an adhesive mixed with an additive that interacts with the adhesive, the plastic upper plate and the glass bottom plate in a manner that strengthens a bond between the plastic upper plate and the glass lower plate.

Abstract: A cardiac stimulator capable of measuring pacing impedance includes a tank capacitor for delivering charge to the heart via device leads, a shunt resistor, and high-impedance buffers for measuring pacing current through the shunt resistor. Soon after the leading edge of the stimulation pulse, the voltage across the shunt resistor, as sampled by a high-impedance buffer, indicates lead and cardiac tissue resistance. Just prior to opening the pacing switch to terminate the stimulation pulse, the voltage across the shunt resistor is sampled by a high-impedance buffer and held once again to allow the capacitance of the lead/heart tissue to be calculated. In alternative embodiments, a high-impedance buffer measures the voltage between the tank capacitor and ground immediately following the stimulation pulse to allow estimation of the lead/heart tissue capacitance.

Abstract: A device and method for detecting a signal from an analytical sample is provided. The device comprises a platen with a first and second surface, a number of collection structures extending from the second surface. Each collection structure has a capillary channel running along a common axis as the collection structure from the platen to a terminal end of the structure. Within the capillary channel are sample analytes of interest. On the first surface is disposed a number of optical elements, corresponding to each collection structure, each centered on a capillary channel. Each collection structure has a geometry that can concentrate an optical emission from within the capillary channel, along the entire length of the capillary channel, towards the first surface and optical elements, to a detection device, by means of either mirrored surface reflection or total internal reflection. The collection structure may also direct a light beam into the capillary channel.

Abstract: A multiwell plate is described that is used for assaying samples and is made from a plastic upper plate which forms the sidewalls of one or more wells and a glass lower plate which forms the bottom walls of the wells. The plastic upper plate and glass lower plate are attached and bound to one another by an enhanced adhesive. The enhanced adhesive includes an adhesive mixed with an additive that interacts with the adhesive, the plastic upper plate and the glass bottom plate in a manner that strengthens a bond between the plastic upper plate and the glass lower plate. Also described herein is a method for making such multiwell plates.

Abstract: A microplate that is manufactured from a thermally conductive material and methods for making and using the microplate are described herein. Basically, the microplate has a series of wells formed within a frame that is manufactured from a thermally conductive material which enables the wells to have relatively rigid walls which in turn makes it easier to handle the microplate. The thermally conductive material can be a metal or a mixture of a polymer (e.g., polypropylene, LCP) and one or more thermally conductive additives (e.g., carbon fiber, metal, ceramic). Also described herein is a tube manufactured from a thermally conductive material and methods for making and using the tube.

Abstract: A cardiac stimulator capable of measuring pacing impedance includes a tank capacitor for delivering charge to the heart via device leads, a shunt resistor, and high-impedance buffers for measuring pacing current through the shunt resistor. Soon after the leading edge of the stimulation pulse, the voltage across the shunt resistor, as sampled by a high-impedance buffer, indicates lead and cardiac tissue resistance. Just prior to opening the pacing switch to terminate the stimulation pulse, the voltage across the shunt resistor is sampled by a high-impedance buffer and held once again to allow the capacitance of the lead/heart tissue to be calculated. In alternative embodiments, a high-impedance buffer measures the voltage between the tank capacitor and ground immediately following the stimulation pulse to allow estimation of the lead/heart tissue capacitance.

Abstract: A cardiac stimulator capable of measuring pacing impedance includes a tank capacitor for delivering charge to the heart via device leads, a shunt resistor, and high-impedance buffers for measuring pacing current through the shunt resistor. Soon after the leading edge of the stimulation pulse, the voltage across the shunt resistor, as sampled by a high-impedance buffer, indicates lead and cardiac tissue resistance. Just prior to opening the pacing switch to terminate the stimulation pulse, the voltage across the shunt resistor is sampled by a high-impedance buffer and held once again to allow the capacitance of the lead/heart tissue to be calculated. In alternative embodiments, a high-impedance buffer measures the voltage between the tank capacitor and ground immediately following the stimulation pulse to allow estimation of the lead/heart tissue capacitance.

Abstract: A multiwell plate is described that is used for assaying samples and is made from a plastic upper plate which forms the sidewalls of one or more wells and a glass lower plate which forms the bottom walls of the wells. The plastic upper plate and glass lower plate are attached and bound to one another by an enhanced adhesive. The enhanced adhesive includes an adhesive mixed with an additive that interacts with the adhesive, the plastic upper plate and the glass bottom plate in a manner that strengthens a bond between the plastic upper plate and the glass lower plate. Also described herein is a method for making such multiwell plates.

Abstract: A cardiac stimulator capable of measuring pacing impedance includes a tank capacitor for delivering charge to the heart via device leads, a shunt resistor, and high-impedance buffers for measuring pacing current through the shunt resistor. Soon after the leading edge of the stimulation pulse, the voltage across the shunt resistor, as sampled by a high-impedance buffer, indicates lead and cardiac tissue resistance. Just prior to opening the pacing switch to terminate the stimulation pulse, the voltage across the shunt resistor is sampled by a high-impedance buffer and held once again to allow the capacitance of the lead/heart tissue to be calculated. In alternative embodiments, a high-impedance buffer measures the voltage between the tank capacitor and ground immediately following the stimulation pulse to allow estimation of the lead/heart tissue capacitance.

Abstract: This invention provides a labeling format for use in assays that require the immobilization of biomolecules on a flat substrate surface. The ceramic labeling indicia of the present invention provide a format that can supply the user with required identification about the substrate and its content without adversely affecting a chemically active surface required for probe immobilization.