Abstract: The invention relates to a cryoapparatus for freezing and/or thawing a sample (1), especially in the cryopreservation of a biological sample (1), with a coolable cooling space (3) and with a sample container (10) arranged in the cooling space (3) for temporarily receiving the sample (1) when freezing or thawing the sample (1). It is suggested that the sample container (10) can be tempered separately from the cooling space. Furthermore, the invention comprises an associated operating method.

Abstract: A method for producing a biological material composition of animal, non-human origin, especially as feed or food, is described. The method includes an aggregation of stem cells from differentiated exocrine glandular tissue of an animal organism to organoid bodies, and a preparation of the material composition from the organoid bodies.

Abstract: Described is a fluidic microsystem with at least one compartment for the receiving and/or the through-flow of a liquid and having an electrode arrangement with a multiplicity of electrodes, between which a coaction zone is established, whereby the compartment possesses at least one wall, through which electromagnetic radiation can be linked into the said coaction zone in accord with a predetermined incident direction, and on at least one electrode a cooling apparatus is provided, and on which at least one respective electrode at least one reflector layer is provided, and wherein the respective electrode is at least partially shielded in reference to the incident beam direction, and having at least one heat conducting layer by means of which the respective electrode stands in thermal communication with a wall of the compartment, and/or includes an active cooling element, which is placed in thermal contact with the respective electrode.

Abstract: The invention relates to a cooling device (1) for cooling an item to be cooled, especially for use in the examination, handling and processing of cryogenic samples. The device includes a cooling compartment (3) for receiving the item to be cooled, an inner wall defining the cooling compartment (3), an outer wall, a space between the outer wall and the inner wall, and a coolant feed line (11) for feeding a coolant. According to the invention, the coolant feed line (11) leads into the space between the inner wall and the outer wall and feeds the coolant into the space, the inner wall being permeable for the coolant.

Abstract: The invention relates to a cooling apparatus, especially for cryogenically preserving biological samples, comprising a duct (5) for delivering a coolant (3) to a cooling chamber (1), a heater (6) that has an adjustable first heating performance (P2) for heating the coolant (3) delivered to the cooling chamber (1), a first temperature sensor (8-10) for measuring the temperature (T2-T4) in the cooling chamber (1), a second temperature sensor (7) for measuring the temperature (T1) of the coolant (3) delivered to the cooling chamber (1), and a regulator (11) for regulating the temperature. Said regulator (11) is embodied as a multiple regulator which detects several temperatures (T1-T4) as control variables and/or adjusts several heating performances (P1, P2) as manipulated variables. The invention further relates to a corresponding operating method.

Abstract: A method for producing cell material (20) having multiple biological cells (21), which have a predefined geometrical arrangement, includes the steps of providing a manipulation tool (10) having a tool body (11), whose surface (12, 14) at least partially contacts the cell material (20), and adjusting the manipulation tool (10) using a change of geometrical properties of the surface (12, 14) in such a way that the geometrical arrangement of the cells (21) is changed. A manipulation tool for performing a method of this type is also described.

Abstract: The invention relates to a sample holding device (100), particularly for cryopreserving at least one sample, which has a bundle (10) consisting of a multitude of tubular flexible sample compartments (11, 12,) and a retaining device (20), to which the bundle (10) of sample compartments is connected. The retaining device (20) has a multitude of retaining frames (21, 22,) that are arranged in a longitudinal direction of the bundle (10).

Abstract: The invention relates to a device and method for handling a probe, in particular, for treating, examining, inserting or extracting a cryoprobe. According to the invention, said probe is surrounded by an ambient gas, during handling, and an air-conditioning device (33) cools, dries and/or at least partially replaces the ambient gas by a protective gas, in order to prevent deterioration of the probe by the ambient gas during handling.

Abstract: Substrates (100) are described for receiving a plurality of samples, especially for the preservation of biological samples at cryotemperatures comprising a plurality of substrate plates (11, 12, 13) arranged on top of one another as a stack (10) and comprising an anchoring axis (20) to which the substrate plates (11, 12, 13) are connected. Processes for the preservation of biological samples at cryotemperatures are also described.

Abstract: A microsystem adapted for dielectrophoretic manipulation of particles in a suspension liquid wherein the microsystem has a channel with channel walls and a longitudinal extension. An electrode arrangement is present which has at least one microelectrode on at least one of the channel walls. This acts to generate a field barrier which crosses the channel at least partly. The microelectrode has a band-shape or has a multitude of straight electrode sections connected to each other. The band-shape has a predetermined curvature or the straight electrode sections are arranged with predetermined different angles so that the field barrier has a predetermined curvature relative to the longitudinal extension of the channel.

Abstract: A method for moving a probe (10, 91) through a cell material (20), which is formed from biological cells (21), is described, the probe (10) displacing the cells (21) without injury. A probe (10, 91) for performing the method and a cell manipulator, which is equipped with at least one probe of this type, are also described.

Abstract: A carrier (10) for at least one specimen chamber (11), in particular for cryoconservation of biological specimens is described, comprising a mounting frame (20) which is set up for positioning the specimen chamber (11) and has a first frame part (30) and a second frame part (40) which can be detachably assembled with connecting elements (50) and are in contact on the side faces (31, 41) in the assembled state, wherein the specimen chamber (11) can be secured between the side faces (31, 41).

Abstract: A cell transfer method is described, in which transfer of at least one biological cell (21) describing a natural cell locomotion between a carrier (80) and a probe (10) is provided, and whereby positioning the probe (10) and the carrier (80) relative to one another in a transfer arrangement is provided, in which transfer of the cell (21) takes place due to the natural cell locomotion.

Abstract: To measure or exert optically-induced forces on at least one particle in the focus of an optical cage, the following steps are taken: a) the focus is positioned in a microelectrode arrangement with a three-dimensional electrical field that has a field gradient which forms an electrical capture area, and the focus is at a distance from the capture are and b) the amplitude of the electrical field, the light power of the light beam forming the optical cage, and/or the distance of the capture area from the focus are varied to detect which varied field property moves the particle from the focus to the capture area or vice versa, or at least to temporarily move the particle into the capture area.

Abstract: A cryostorage device (100) is described that includes at least one data storage device (200) and at least one sample receptacle device (300) with at least one sample chamber (301, 302, etc.) for the uptake of a suspension sample, the at least one sample chamber (301, 302, etc.) being connected to the data storage device (200) and having a elongated, hollow shape that extends from an inlet end (320) over a predetermined length to an outlet end (330) and being attached to the at least one data storage device (200) in a flexible and movably hanging manner.

Abstract: With a method for cryo-preservation, at least one specimen is arranged on a storage substrate and specimen data, which are characteristic for features of the specimen, are stored at specific positions. Also, a storage substrate for cryo-preservation with such a method is described.

Abstract: A method for operating a cryostorage device (100), especially for biological samples, is described which comprises a sample carrier (10) to receive at least one sample (11) and a data storage (20), wherein data are inductively transmitted from the data storage device (20) into a wireless transmission channel (40) and/or conversely using a resonant circuit (30) connected to the data storage device (20).

Abstract: A cryosubstrate, in particular for cryopreservation of biological specimens, having at least one solid-phase element is described. This solid-phase element is equipped for adherent fixation of specimens, with the solid-phase element comprising at least one carrier filament. Furthermore, a method of cryopreservation of biological specimens using such a cryosubstrate is described.

Abstract: For the cell trace based testing of biological cells, wherein the cells (16) are applied to an at least partially structured and/or surface modified substrate (11) and move adhesively over surface track regions (13, 15) of the substrate while producing cell traces (14a, 14b) consisting of material residues separated from the cells, cell tests are performed on the cell traces. A process for cell cultivation on biocompatible modified substrates whose surfaces are covered by cell traces is also described.

Abstract: In order to isoelectrically separate particles with a pH-dependent net charge, the particles are exposed in a guiding liquid to electric field forces. The pH value of the guiding liquid is set in such a way that at least one predetermined type of particle is separated from the remaining particles and migrates to a fixing collecting means under the effect of the electric field forces. The collecting means is for example a porous hollow fiber delimited by electrodes which generate the electric field forces and crossed by the guiding liquid together with the sample to be separated. The particles whose isoelectric point matches the pH value of the guiding liquid run unimpeded through the fibers, whereas the remaining particles are pressed against the inner wall of the fiber and are prevented from being carried away with the liquid flow.