Abstract: Methods and apparatuses for determining the depth and concentration of fluorophores in a turbid medium are disclosed. The method advantageously provides for a rapid estimation of the depth of the fluorophore using characteristics of a temporal point spread function. The concentration of the fluorophore can be determined using the method of the present invention by combining a calculated depth of the fluorophore with a measurement of the intensity of the emitted fluorescence. The intensity can be accurately measured by the apparatus disclosed herein which combine back-reflection and trans-illumination geometries for the source of light injecting and detection.

Abstract: Methods and apparatuses for determining the depth and concentration of fluorophores in a turbid medium are disclosed. The method advantageously provides for a rapid estimation of the depth of the flurophore using characteristics of a temporal point spread function. The concentration of the flurophore can be determined using the method of the present invention by combining a calculated depth of the flurophore with a measurement of the intensity of the emitted fluorescence. The intensity can be accurately measured by the apparatus disclosed herein which combine back-reflection and trans-illumination geometries for the source of light injecting and detection.

Abstract: There is provided an improved method for enhancing fluorescence images of an object, such as a biological tissue, by selectively eliminating or reducing unwanted fluorescence from fluorophores other than the fluorophore of interest. The method is based on the measurement of the lifetime of fluorophores while preserving information related to the fluorescence intensity of the fluorophore of interest.

Abstract: The present invention relates to a method of decomposition of a test sample into constituents thereof. The method proceeds by optically imaging the test sample to obtain a corresponding unknown time-domain resolved signal and decomposes the unknown time-domain resolved signal by comparing the unknown time-domain resolved signal with time-domain resolved reference signals. Furthermore, the method allows the determination of the presence or absence of constituents. Relative quantities may also be determined if sample material properties are known or taken into account. Lifetime decay of the constituents may also be estimated by handling effect of light diffusion in the test sample as time decay.

Abstract: Methods and apparatuses for determining the depth and concentration of fluorophores in a turbid medium are disclosed. The method advantageously provides for a rapid estimation of the depth of the flurophore using characteristics of a temporal point spread function. The concentration of the flurophore can be determined using the method of the present invention by combining a calculated depth of the flurophore with a measurement of the intensity of the emitted fluorescence. The intensity can be accurately measured by the apparatus disclosed herein which combine back-reflection and trans-illumination geometries for the source of light injecting and detection.

Abstract: There is provided an improved method for enhancing fluorescence images of an object, such as a biological tissue, by selectively eliminating or reducing unwanted fluorescence from fluorophores other than the fluorophore of interest. The method is based on the measurement of the lifetime of fluorophores while preserving information related to the fluorescence intensity of the fluorophore of interest.